Multiplicação in vitro de plantas juvenis de mamoeiro

Vidal, Francisco Ronaldo; Diniz, Josefa Diva Nogueira; Silva, Fanuel Pereira da

doi:10.1590/s1983-40632013000100010

Cited by 7 publications

(7 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Micropropagated woody species generally have a low multiplication rate as observed in Libidibia ferrea, which the highest rate (27.75) was obtained when the explants were grown in the presence of 3.96 µM of BAP (Silva, 2019). Although BAP is one of the most widely used phytoregulators to increase the in vitro multiplication rate, this cytokinin does not always have a positive effect for all species (Vidal et al, 2013), a statement that corroborates with the data obtained in the present study with E. involucrata. This result confirms therefore the importance of adjusting the propagation protocols in order to increase the mass production of plants (Silva, 2019).…”

Section: Resultssupporting

confidence: 92%

6-Benzylaminopurine and 3-Indolebutyric acid on the in vitro multiplication of Eugenia involucrata

2021

View full text Add to dashboard Cite

Eugenia involucrata is an important Brazilian forest species, which it can be used for timber, such as firewood and charcoal, and non-timber purposes, for culinary and medicinal use. Considering the limitations of its seminal reproduction, vegetative propagation, particularly by micropropagation, becomes an alternative for plantlets production. In this study we evaluated the effect of the phytoregulators 6-benzylaminopurine (BAP) and 3-indolebutyric acid (IBA) on the in vitro multiplication stage of nodal segments. The experiment was carried out in a completely randomized design, in a 3 x 4 factorial array, the main factors were BAP (0; 0.1 or 0.2 µM) and IBA (0; 10; 20 or 30 µM). After 60 days of in vitro inoculation, evaluations on the the number of buds, number of shoots, number of leaves, callus formation (%) and number of roots per explant were carried out. BAP had not significant effect, however, the use of IBA alone favours the callogenesis and negatively affected leaves, shoots and roots formation. A moderate multiplication rate was obtained when considering in vitro bud formation. BAP and IBA are dispensable in in vitro multiplication of E. involucrata.

show abstract

Section: Resultssupporting

confidence: 92%

6-Benzylaminopurine and 3-Indolebutyric acid on the in vitro multiplication of Eugenia involucrata

2021

View full text Add to dashboard Cite

show abstract

“…The addition of plant growth regulators in the culture media compensate for any possible deficiencies of the hormonal endogenous levels in the explants (VIDAL et al, 2013). Among the plant growth regulators, gibberellins show physiological activities in the induction of seeds germination and in breaking dormancy.…”

Section: Introductionmentioning

confidence: 99%

Germinação in vitro de variedades de rosa-do-deserto

Varella

Silva

Cruz

et al. 2015

View full text Add to dashboard Cite

The drought stress resistance is a characteristic of the desert rose and its estimable beauty flowers, which gave it great relevance in the ornamental market. However, the desert rose production and germination is hampered by possible sterility of their male and female flowers and frequent problems in pollination, so the tissue culture is a promising alternative to the propagation of these plants. This study aimed to evaluate the effect of gibberellic acid on four commercial varieties of desert rose (Adenium obesum) cultivated in vitro. The seeds of the varieties 'Orange Pallet', 'Carnation violet', 'Diamond ring' and 'Vermiliont' were sterilized and inoculated on Water + Agar (T0), medium MS (T1), ½ MS (T2), MS + 0.25 mg L -1 GA 3 (T3), MS + 0.5 mg L -1 GA 3 (T4), ½ MS + 0.25 mg L -1 GA 3 (T5), ½ MS 0.5 mg L -1 GA 3 (T6). The seeds germination of A. obesum was initiated on the fourth day of cultivation and on the tenth day was possible to observe the expansion of the cotyledons and leaf expansion with subsequent development of early secondary root. The 'Orange pallet' variety germinated 100% of seeds on water + agar and MS ½ + 0.5 mg L -1 of GA 3 . For 'Diamond Ring' and 'Carnation violet' the highest rate of germination occurred in treatments MS ½; 0.25 mg L -1 GA 3; MS + 0.5 mg L -1 GA 3 MS ½ + 0.5 mg L -1 GA 3 averaging 80% and 70%, respectively. For 'Vermiliont' the best response was in MS and MS ½ + 0.5 mg L -1 GA 3 ranging between 70-90% germinated embryos. It was registered different malformations in all treatments like absence of roots and apexes during seedling development. The concentrations of GA 3 did not affect significantly the seed germination.

show abstract

“…(Moura, Titon, Miranda, Moreira, & Oliveira, 2012) and Hancornia speciosa Gomes (Oliveira, Freire, & Aloufa, 2016), whose explants were more responsive in MS culture medium devoid of BAP and NAA. Vidal, Diniz and Silva (2013) found that BAP, despite being widely used in the in vitro multiplication phase, may not exert a satisfactory effect, depending on the species studied. Mallón, Rodríguez-Oubina and González (2011) attributed this behavior to the concentrations of endogenous auxins and cytokinins being sufficient to induce plant development in vitro.…”

Section: Resultsmentioning

confidence: 98%

In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations

Santos

Silveira

Souza

et al. 2021

Acta Sci. Biol. Sci.

View full text Add to dashboard Cite

In vitro multiplication is an important tissue culture technique that is capable of efficiently producing seedlings at any scale. It is a propagation method based on the aseptic culture of small propagules in a suitable culture medium to enable plant regeneration. Multiplication experiments conducted in vitro to set protocols adapted to wild Manihot species have used modified mineral salts and MS vitamins as basic culture medium. Here, 25 treatments based on combinations of the regulators benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) at 0, 0.025, 0.05, 0.075, and 0.1 mg L-1 were used for in vitro multiplication of three genotypes of wild Manihot species (M. violaceae Pohl Müll. Arg., M. pseudoglaziovii Pax & Hoff., and M. flabellifolia Pohl). Plant height and the number of 1 cm minicuttings, number of roots, shoots, green leaves and senescent leaves were recorded 120 days after explant inoculation. M. violaceae Pohl. Müll. Arg. and M. flabellifolia Pohl. presented favorable results with 0.05 and 0.025 mg L-1 NAA, respectively. Culture medium lacking NAA and BAP favored the in vitro growth of M. pseudoglaziovii Pax & Hoff.

show abstract

Multiplicação in vitro de plantas juvenis de mamoeiro

Cited by 7 publications

References 10 publications

6-Benzylaminopurine and 3-Indolebutyric acid on the in vitro multiplication of Eugenia involucrata

6-Benzylaminopurine and 3-Indolebutyric acid on the in vitro multiplication of Eugenia involucrata

Germinação in vitro de variedades de rosa-do-deserto

In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations

Contact Info

Product

Resources

About