Implantation of platelet-rich fibrin and cartilage granules facilitates cartilage repair in the injured rabbit knee: preliminary report

Kuo, Ting‐Shen; Lin, Muh-Shi; Lin, Yu-Chen; Lin, Ying; Su, Rou Jen; Lin, Hui Wen; Chan, Wing P.

doi:10.1590/s1807-59322011001000026

Cited by 23 publications

(25 citation statements)

References 13 publications

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“…The average defect-filling rate in the control group ranged from 50 to 75%. In a previous study, Kuo et al [24] showed that PRF improved the healing of cartilage defects. It is desirable to have a defect filled with cartilage with smooth healing.…”

Section: Discussionmentioning

confidence: 96%

Regenerative effect of platelet-rich fibrin on articular cartilage defects in an experimental rat model

Metineren

Dülgeroğlu

2019

The European Research Journal

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Objectives: Different materials are currently being used experimentally to accelerate cartilage healing and to obtain hyaline cartilage. We aimed to investigate the effect of platelet-rich fibrin on joint cartilage healing. Methods: Twenty rats were used in this study. platelet-rich fibrin was obtained from 4 rats, and the remaining 16 rats were randomly divided into two groups (4 and 8 weeks). Both knee joints were prepared, and an osteochondral defect was created at the femoral condyle in all rats. platelet-rich fibrin was placed into the osteochondral defect of the right knee, while the left knee joint was used as a control. Groups were sacrificed after 4 and 8 weeks, respectively. Results: According to the O'Driscoll score, there were no significant differences between the right and left knee scores in group 4 weeks (p = 0.820). However, the right knee scores were significantly different than the left knee scores in group 8 weeks (p < 0.001). Defects were completely filled with cartilage tissue after 8 weeks in the platelet-rich fibrin group. Conclusions: Similar to its role in various tissues, platelet-rich fibrin is an effective biomaterial that enhances the healing of osteochondral defects.

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Section: Discussionmentioning

confidence: 96%

Regenerative effect of platelet-rich fibrin on articular cartilage defects in an experimental rat model

Metineren

Dülgeroğlu

2019

The European Research Journal

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“…Studies on healing bone have shown that PRFM caused fibroblastic proliferation and supported its osteoblastic activity by triggering angiogenesis . In their study on rabbits, Kuo et al created cartilage defects in rabbits and compared PRFM group to the control group . The authors reported significant healing in PRFM implanted rabbits based on the results of MR and histological observation.…”

Section: Discussionmentioning

confidence: 99%

“…PRP has several different growth factors and other cytokines that stimulate healing of bone . It has been used for different purposes in surgery for approximately 30 years and conceivably increases the survival of cartilage grafts used in rhinoplasty …”

Section: Introductionmentioning

confidence: 99%

Efficacy of platelet-rich fibrin matrix on viability of diced cartilage grafts in a rabbit model

et al. 2014

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“…No additional activation steps are required, and the release of growth factors from PRF can be steadily sustained [24]. The effects of PRF in facilitating rabbit meniscal and cartilage repairs have been documented in [6,[25][26][27] however, the relatively small joint size, thin cartilage layer, and great potential for intrinsic healing have limited the translational value for this one-stage cartilage treatment method.…”

Section: Introductionmentioning

confidence: 99%

Platelet-Rich Fibrin Facilitates One-Stage Cartilage Repair by Promoting Chondrocytes Viability, Migration, and Matrix Synthesis

Wong

Lin

et al. 2020

IJMS

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The main aim of this study is to develop a one-stage method to combine platelet-rich fibrin (PRF) and autologous cartilage autografts for porcine articular cartilage repair. The porcine chondrocytes were treated with different concentrations of PRF-conditioned media and were evaluated for their cell viability and extracellular glycosaminoglycan (GAG) synthesis during six day cultivation. The chemotactic effects of PRF on chondrocytes on undigested cartilage autografts were revealed in explant cultures. For the in vivo part, porcine chondral defects were created at the medial femoral condyles of which were (1) left untreated, (2) implanted with PRF combined with hand-diced cartilage grafts, or (3) implanted with PRF combined with device-diced cartilage grafts. After six months, gross grades, histological, and immunohistochemical analyses were compared. The results showed that PRF promotes the viability and GAG expression of the cultured chondrocytes. Additionally, the PRF-conditioned media induce significant cellular migration and outgrowth of chondrocytes from undigested cartilage grafts. In the in vivo study, gross grading and histological scores showed significantly better outcomes in the treatment groups as compared with controls. Moreover, both treatment groups showed significantly more type II collagen staining and minimal type I collagen staining as compared with controls, indicating more hyaline-like cartilage and less fibrous tissue. In conclusion, PRF enhances the viability, differentiation, and migration of chondrocytes, thus, showing an appealing capacity for cartilage repair. The data altogether provide evidences to confirm the feasibility of a one-stage, culture-free method of combining PRF and cartilage autografts for repairing articular cartilage defects. From translational standpoints, these advantages benefit clinical applications by simplifying and potentiating the efficacy of cartilage autograft transplants.

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Implantation of platelet-rich fibrin and cartilage granules facilitates cartilage repair in the injured rabbit knee: preliminary report

Cited by 23 publications

References 13 publications

Regenerative effect of platelet-rich fibrin on articular cartilage defects in an experimental rat model

Regenerative effect of platelet-rich fibrin on articular cartilage defects in an experimental rat model

Efficacy of platelet-rich fibrin matrix on viability of diced cartilage grafts in a rabbit model

Platelet-Rich Fibrin Facilitates One-Stage Cartilage Repair by Promoting Chondrocytes Viability, Migration, and Matrix Synthesis

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