Chemical composition of the essential oil from Kelussia odoratissima Mozaff. and the evaluation of its sedative and anxiolytic effects in mice

Abstract

OBJECTIVE:The present study aimed to evaluate the sedative and anxiolytic effects of the essential oils and hydroalcoholic extract of Kelussia odoratissima Mozaff. (K. odoratissima) in mice by utilizing an elevated plus maze. The chemical composition of its essential oil was also determined.METHODS:The hydroalcoholic extract or essential oil fraction from this plant were administered intraperitoneally to male mice at various doses 30 min before testing. The anxiolytic and sedative effects were determined by an elevated plus maze and locomotor activity tests, respectively.RESULTS:According to the results, none of the administered doses of hydroalcoholic extract or essential oil fraction of K. odoratissima changed the percentage of the time spent or number of entries into the open arms of the elevated plus maze. In contrast, the cumulative spontaneous locomotor activity of mice treated with the essential oil or hydroalcoholic extract was significantly decreased. Chemical analysis of the essential oil by Gas chromatography-mass spectromentry (GC-MS) showed that 3-butylidene-4,5-dihydrophthalide (85.9%) was the major component.CONCLUSION:These data confirm the sedative properties of K. odoratissima, yet there were no profound anxiolytic effects observed.

Chemical composition and antioxidant activity of essential oils of three endemic medicinal plants of Iran

“…Results of other study (Aklaghi 2008) indicated the flower, stem, and leaves of F. angulata (collected from east-north, Iran) yielded 0.66, 0.54 and 0.43% (w/w), respectively. In agreement with previously published reports, Rabbani et al (2011) reported that the aerial parts of K. odoratissima yielded a 0.4% (v/w). The results of a study (Sonboli et al 2006) indicated the EO yield of the aerial parts (leaves and stem) of T. lasiopetalum (synonym: H. lasiopetalum) collected from west Iran was 0.03% (w/w) based on the dry weight of plant.…”

“…Aklaghi (2008) reported that α-phellandrene and α-pinene are main compositions in flowers, stems, and leaves of F. angulata. In total, 38 compounds in the EO from K. odoratissima were identified; the major compounds were α-pinene (20.1%), 1,8-cineole (18.2%), and Z-ligustilide (15.5%), while the results of other reports indicated that the main components of the EO of K. odoratissima were 3-n butyl phtalide and Z-ligustilide (Rabbani et al 2011;Ghasemi Pirbalouti et al 2012;Sajjadi et al 2013). Various factors, including ecotype, harvesting stage, drying, and extraction methods caused on this variation.…”

“…All these three species are endemic to Iran and their description, distribution and ethnobotanical properties having been described elsewhere (Ghasemi Pirbalouti 2009, 2010, Javidnia et al 2006, Mozaffarian 2008Rabbani et al 2011).…”

Isolation and Identification of Ferulic Acid From Aerial Parts of Kelussia odoratissima Mozaff

“…Volatile constituents of K. odoratissima were reported by the authors in advance. The main constituents of the volatile oil are phthalides with z-ligustilide as the major constituent (11). However, to the best of authors knowledge, this paper was the first report on nonvolatile components of K. odoratissima.…”

Effect of Kelussia odoratissima Mozaffarian on Lipid and Glucose Profile

“…In addition, it is traditionally used as a medicinal plant to treat hypertension, inflammation, ulcers and cardiovascular diseases (53). There is limited literature on the pharmacological properties of this plant, yet recent studies have shown that the hydro-alcoholic extract of Kelussia odoratissima is sedative (54), which may be due to its bioactive phthalides (55). Shojaei Asadiyeh et al in 2011 showed that major ingredients of the aerial parts of Kelussia odoratissima are Z-ligustilide and 3-N-butylphthalide (50).…”