Limitações do uso do fragmento mtp40 como marcador de diferenciação entre Mycobacterium tuberculosis e M. bovis

Viana-Niero, Cristina; Leão, Sylvia Cardoso

doi:10.1590/s1806-37132004000400018

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…Of the 28 isolates identified as M. tuberculosis in the present study, only 3 were found to be resistant to pyrazinamide. In making the differential diagnosis between M. tuberculosis and M. bovis, it is advisable to use not only phenotypic tests but also genotypic markers, (14) and various molecular biology techniques have been used successfully in the characterization of M. tuberculosis isolates.…”

Section: Brief Communicationmentioning

confidence: 99%

Identificação de Mycobacterium bovis em cepas micobacterianas isoladas espécimes clínicos humanos em um complexo hospitalar na cidade do Rio de Janeiro

et al. 2011

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In 2005 and 2006, 8,121 clinical specimens submitted to the Mycobacteriology Laboratory of the Clementino Fraga Filho University Hospital/Thoracic Diseases Institute, in the city of Rio de Janeiro, Brazil, were inoculated on Löwenstein-Jensen medium containing glycerol and pyruvate. There were 79 mycobacteria isolates that presented growth only on pyruvate-containing medium, and those isolates were selected for the presumptive identification of Mycobacterium bovis. The selected isolates were screened with biochemical tests, PCR amplification (with the specific primers Rv0577 and Rv1510), and pyrazinamide susceptibility tests. All of the strains isolated showed specific phenotypical and genotypical patterns characteristic of M. tuberculosis, and no M. bovis strains were detected. Keywords

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Section: Brief Communicationmentioning

confidence: 99%

Identificação de Mycobacterium bovis em cepas micobacterianas isoladas espécimes clínicos humanos em um complexo hospitalar na cidade do Rio de Janeiro

et al. 2011

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“…fortuitum, podem produzir quadro semelhante à tuberculose. No entanto são consideradas oportunistas e não tuberculosas (Viana-Niero e Leão, 2004;Ernst et al, 2007).…”

Section: Agente Etiológicounclassified

Estudo da resposta imunológica de anticorpos IgG, IgM e IgA, subclasses de IgG (IgG1 e IgG3) e avidez de IgG, por Western Blotting, em amostras de soros de pacientes com tuberculose pulmonar e comparação de resultados com métodos microbiológicos e dosagem de interferon-gama

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DNA markers for tuberculosis diagnosis

et al. 2018

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Tuberculosis (TB), caused by Mycobacterium tuberculosis complex (MTBC), is an infectious disease with more than 10.4 million cases and 1.7 million deaths reported worldwide in 2016. The classical methods for detection and differentiation of mycobacteria are: acid-fast microscopy (Ziehl-Neelsen staining), culture, and biochemical methods. However, the microbial phenotypic characterization is timeconsuming and laborious. Thus, fast, easy, and sensitive nucleic acid amplification tests

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Limitações do uso do fragmento mtp40 como marcador de diferenciação entre Mycobacterium tuberculosis e M. bovis

Cited by 5 publications

References 12 publications

Identificação de Mycobacterium bovis em cepas micobacterianas isoladas espécimes clínicos humanos em um complexo hospitalar na cidade do Rio de Janeiro

Identificação de Mycobacterium bovis em cepas micobacterianas isoladas espécimes clínicos humanos em um complexo hospitalar na cidade do Rio de Janeiro

Estudo da resposta imunológica de anticorpos IgG, IgM e IgA, subclasses de IgG (IgG1 e IgG3) e avidez de IgG, por Western Blotting, em amostras de soros de pacientes com tuberculose pulmonar e comparação de resultados com métodos microbiológicos e dosagem de interferon-gama

DNA markers for tuberculosis diagnosis

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