Two new species of Pseudancistrus, a genus diagnosed by non-evertible cheek plates and hypertrophied odontodes along the snout margin, are described from two drainages of the Brazilian Shield: Pseudancistrus
kayabi from the rio Teles Pires (rio Tapajós basin) and Pseudancistrus
asurini from the rio Xingu. The new species are distinguished from congeners (Pseudancistrus
barbatus, Pseudancistrus
corantijniensis, Pseudancistrus
depressus, Pseudancistrus
nigrescens, Pseudancistrus
reus, and Pseudancistrus
zawadzkii) by the coloration pattern. Pseudancistrus
kayabi has dark bars on the dorsal and caudal fins which are similar to that of Pseudancistrus
reus from the Caroní River, Venezuela. Pseudancistrus
asurini is unique among Pseudancistrus in having whitish tips of the dorsal and caudal fins in juveniles to medium-sized adults.