Antioxidant effect of Morus nigra on Chagas disease progression

Abstract

Considering the widespread popular use of Morus nigra and the amount of scientific information on its antioxidant and anti-inflammatory activity, the effectiveness of this phytotherapeutic compound in the parasitemia progression during the acute phase of Chagas disease and its role in the development of the inflammatory process as well as its effects on the oxidative damage in the chronic phase of infection were evaluated. Thus, 96 male Swiss mice were randomly divided into eight groups, four groups were uninfected controls, and four groups were intraperitoneally infected with 5.0 x 104 blood trypomastigotes forms of T. cruzi QM2 strain. Four batches composed of one uninfected and one infected group were respectively treated with 70% alcohol solution and 25 μL, 50 μL and 75 μL of the phytotherapeutic compound. Levels of antioxidant elements (TBARS, FRAP, GSH and Sulfhydryl groups) were measured in plasma samples. The phytotherapeutic compound’s antioxidant activity was measured by polyphenol and total flavonoid quantification, DPPH, NO, and FRAP method. Our results showed that the vehicle influenced some of the results that may have physiological relevance in Chagas disease. However, an important action of M. nigra tincture was observed in the progression of Chagas disease, since our results demonstrated a reduction in parasitemia of treated groups when compared to controls, especially in the group receiving 25 µL. However, in the chronic phase, the 50-µL dosage presented a better activity on some antioxidant defenses and minimized the tissue inflammatory process. Results indicated an important action of M. nigra tincture on the Chagas disease progression.

Manufacture of Hard Gelatin Capsules From a Lyophilisate of the Morus Nigra Fruit

“…Therefore, it is due to the widespread use of the blackberry fruit for various purposes, that the study of the composition of blackberry fruit has been taken into greater consideration [2]. Since several studies have been found in which the physicochemical characteristics of blackberry fruit have been investigated in recent years [17,18], however, there is no recent study that shows the physicochemical composition and pharmaceutical stability of hard gelatin capsules of a lyophilisate of the M. nigra L. fruit. Therefore, it is rea This study was conducted on the physicochemical composition of the pharmaceutical stability of capsules from the lyophilisate of M. nigra.…”

Potential Role of Antioxidants as Adjunctive Therapy in Chagas Disease

“…Phytotherapy Compounds. The antioxidant potential of phenolic compounds (flavonoids) in the chronic phase was evaluated using the Morus nigra plant extract [90]. Unlike the other studies discussed above, the plant extract was not composed of a specific purified phenolic compound.…”

Pharmacological Properties of Morus nigra L. (Black Mulberry) as A Promising Nutraceutical Resource

“…Numerous researches have proven antioxidant properties of M. nigra with different in vitro methods, including DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay [ 15 , 23 , 24 , 25 , 26 , 28 , 61 , 70 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 ], ABTS (2,2’-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid) radical scavenging assay [ 15 , 91 , 94 , 99 , 100 , 101 , 102 , 103 , 107 , 110 , 112 , 113 , 114 , 115 , 116 , 117 ], reducing power assay [ 15 , 99 , 113 , 118 , 119 ], superoxide anion radical (O2 − ) scavenging assay [ 15 …”