2017
DOI: 10.1590/s1678-9946201759038
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Abstract: Traditional diagnostic methods used to detect American Tegumentary Leishmaniasis, such as histopathology using biopsy samples, culture techniques, and direct search for parasites, have low sensitivity and require invasive collection procedures. This study evaluates the efficiency of noninvasive sampling methods (swab) along with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis using skin and mucous samples from 25 patients who had tested positive for leishmaniasis. The outcome … Show more

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Cited by 18 publications
(20 citation statements)
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“…Forl ow concentrations ( Figure 7B)8 8% of the swab samples produced ah igh signal-to-background ratio (close to 2000:1). [46] In order to be useful in the food industry,s everal strains of Salmonella need to be detected, therefore we show that CLSP can detect avariety of strains of Salmonella (see Supporting Information, Figure S6). We attribute this fact to the typically low recovery rates of swabs, which we measured to be between 1-2 %( data not shown).…”
Section: Angewandte Chemiementioning
confidence: 75%
“…Forl ow concentrations ( Figure 7B)8 8% of the swab samples produced ah igh signal-to-background ratio (close to 2000:1). [46] In order to be useful in the food industry,s everal strains of Salmonella need to be detected, therefore we show that CLSP can detect avariety of strains of Salmonella (see Supporting Information, Figure S6). We attribute this fact to the typically low recovery rates of swabs, which we measured to be between 1-2 %( data not shown).…”
Section: Angewandte Chemiementioning
confidence: 75%
“…We attribute this fact to the typically low recovery rates of swabs, which we measured to be between 1–2 % (data not shown). This makes it likely that swabbing a low number of bacteria from a stainless‐steel plate will result in some samples not containing any Salmonella for enrichment and later for enzyme activity detection . In order to be useful in the food industry, several strains of Salmonella need to be detected, therefore we show that CLSP can detect a variety of strains of Salmonella (see Supporting Information, Figure S6).…”
Section: Figurementioning
confidence: 84%
“…Valencia et al [33] analysed different types of PCR (kDNA of lesion scrapings, imprint of lesion on filter paper, CerviSoftH and HistobrushH) and found high sensitivity and specificity values, especially in kDNA of Cer-viSoftH PCR. Boni et al [10] showed that PCR-kDNA test is more sensible than PCR-HSP70 and qPCR tests and that using swabs is an alternative to conventional biopsy-based methods. The study by Romero et al [52] was not included in meta-analyses of specificity because it did not inform the total number of individuals studied.…”
Section: Results Not Included In the Meta-analysesmentioning
confidence: 99%
“…The opposite occurs with tests that aim to visualise the parasite, which present high specificity and low sensitivity. Molecular diagnoses have also been used, although their utilisation is limited by the need for specialised centres to perform the technique, as well as by their high cost [8][9][10]. Figure 1 describes the clinical path of this review.…”
Section: Introductionmentioning
confidence: 99%