2012
DOI: 10.1590/s1678-91992012000200009
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Abstract: Leishmaniasis is a neglected disease endemic in five continents. It is a severe disease that may lead to death, and its early detection is important to avoid severe damage to affected individuals. Molecular methods to detect Leishmania are considered alternatives to overcome the limitations presented by conventional methods. The aim of this study was to develop multiplex PCR systems able to detect small amounts of target DNA of Leishmania infantum and Leishmania braziliensis, and the gene coding for glyceralde… Show more

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Cited by 15 publications
(25 citation statements)
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“…Indeed, . NTC no template control the use of IC has been proven very important to assess sample quality [13], which is fundamental for obtaining an accurate final molecular diagnosis. Incidentally, the application of external controls has been done in different kinds of sample and for distinct purposes [19,20].…”
Section: Discussionmentioning
confidence: 99%
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“…Indeed, . NTC no template control the use of IC has been proven very important to assess sample quality [13], which is fundamental for obtaining an accurate final molecular diagnosis. Incidentally, the application of external controls has been done in different kinds of sample and for distinct purposes [19,20].…”
Section: Discussionmentioning
confidence: 99%
“…The amplification of mammalian glyceraldehyde-3-phosphate dehydrogenase (G3PD) gene was chosen as IC to determine DNA degradation and PCR inhibitors present in the blood samples [13].…”
Section: Internal Control (Ic)mentioning
confidence: 99%
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“…with high sensitivity and specificity [5-7]. Additionally, the possibility of combining multiple targets in the same assay enables the identification of parasites to the species level, the evaluation of sample integrity and also PCR performance on pools of phlebotomine sandflies [8-12].…”
Section: Introductionmentioning
confidence: 99%