Cross-neutralization of the coagulant activity of Crotalus durissus terrificus venom from the northeast of Argentina by bivalent bothropic antivenom

Abstract

Cross-neutralization of Crotalus durissus terrificus venom coagulant activity was tested using bivalent horse antivenom against Bothrops alternatus and Bothrops diporus venoms. Our in vitro and in vivo experiments showed that bothropic antivenom neutralizes the thrombin-like activity of crotalic snake venom and this cross-reaction was demonstrated by immunoassays either with whole venom or a purified thrombin-like enzyme. These results suggest common antigenic properties and, consequently, similar molecular structure among venom thrombin-like enzymes. Besides, they provide information that could be further used in the development of new antivenom formulations

P9a(Cdt-PLA2) from Crotalus durissus terrificus as good immunogen to be employed in the production of crotalic anti-PLA2 IgG

“…The plates were washed and processed as described by 256 (Rodríguez et al, 2012). Absorbance was read at 490 nm with a Multiskan® EX (Thermo 257 Scientific, USA) multiwell plate reader.…”

An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems

“…This mixture exhibits values of specific proteolytic activity and MiHD sufficient for use as a sensitizing agent for antiserum manufacturing. This will contribute to the obtainment of more specific anti-sera and therefore the avoidance of adverse effects (40). The presence of a low PEG concentration (close to 3 % w/w) in the bottom phase does not represent a disadvantage since this polymer possesses a low immunogenic capability (41).…”