DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis

Corvalan, FH; Sampaio, Rnr; Brustoloni, YM; Andreotti, Renato; Júnior, Lima

doi:10.1590/s1678-91992011000100013

Cited by 10 publications

(10 citation statements)

References 10 publications

(6 reference statements)

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“…PCR assay but negative in the PCR assay for L. donovani complex could be infected by other Leishmania spp. species, i.e., L. braziliensis or L. amazonensis, which have been reported previously in the state of Mato Grosso do Sul (DORVAL et al, 2006;CORVALAN et al, 2011).…”

Section: Methodssupporting

confidence: 56%

“…(VEXENAT et al, 1996;LUCIANO et al, 2009). In our study, cross-reactivity between dog serum samples positive to T. cruzi and L. braziliensis could not be discarded, since the occurrence of T. cruzi and L. braziliensis among dogs is documented in the state of Mato Grosso do Sul (POMPILIO et al, 2005;SAVANI et al, 2009;SOUZA et al, 2009;CORVALAN et al, 2011). One dog that tested positive in the Leishmania spp.…”

Section: Methodsmentioning

confidence: 94%

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Molecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantumin Mato Grosso do Sul, Brazil

Sousa

André

Herrera³

et al. 2013

Rev. Bras. Parasitol. Vet.

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Tick-borne pathogens affect a wide range of vertebrate hosts. To identify tick-borne pathogens among dogs from Campo Grande, MS, Brazil testing seropositive for Leishmania infantum (syn. L. chagasi), a serological and molecular study was conducted to detect Ehrlichia canis, Anaplasma platys and Babesia vogeli in 60 serum and spleen samples. A confirmatory diagnosis of L. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to assess the identity of the parasite species infecting these animals. IgG antibodies to Ehrlichia spp., B. vogeli and L. infantum were found, respectively, in 39 (65%), 49 (81.6%) and 60 (100%) of the sampled dogs. Twenty-seven (45%), fifty-four (90%), fifty-three (88.3%), two (3.3%) and one (1.6%) dog were positive, respectively, for E. canis, Leishmania spp., Leishmania donovani complex, Babesia sp. and Anaplasma sp. in PCR assays. After sequencing, the amplicons showed 99% of identity with E. canis, B. vogeli, A. platys and Leishmania chagasi isolates. The findings of this study indicate that L. infantum-seropositive dogs from Campo Grande are exposed to multiple tick-borne pathogens, which should therefore be included in the differential diagnosis of dogs with clinical suspicion of leishmaniasis.Keywords: Ehrlichia canis, Babesia vogeli, Anaplasma platys, Leishmania infantum, dogs, co-infection. ResumoPatógenos transmitidos por carrapatos atingem uma variedade de hospedeiros vertebrados. Para identificar os agentes patogênicos transmitidos por carrapatos entre cães soropositivos para Leishmania infantum no município Campo Grande-MS, foi realizado um estudo sorológico e molecular para a detecção de Ehrlichia canis, Anaplasma platys e Babesia vogeli em 60 amostras de soro e baço, respectivamente. Adicionalmente, foi realizado o diagnóstico confirmatório de L. infantum por meio de técnicas sorológicas e moleculares. Também foi realizado o alinhamento e análise filogenética das sequências para indicar a identidade das espécies de parasitas que infectam esses animais. Anticorpos IgG anti-Ehrlichia spp., anti-B. vogeli e anti-L. infantum foram detectados em 39 (65%), 49 (81,6%) e 60 (100%) dos cães amostrados, respectivamente. Vinte e sete (45%), cinquenta e quatro (90%), cinquenta e três (88,3%), dois (3,3%) e um (1,6%) cães mostraram-se positivos na PCR para E. canis, Leishmania spp., Leishmania donovani complex, Babesia sp. e Anaplasma sp., respectivamente. Após o seqüenciamento, os amplicons mostraram 99% de similaridade com isolados de E. canis, B. vogeli e A. platys e Leishmania chagasi. Os resultados deste estudo indicaram que os cães soropositivos para L. infantum de Campo Grande, MS, são expostos a vários agentes transmitidos por carrapatos, e, portanto, devem ser incluídos no diagnóstico diferencial em cães com suspeita clínica de leishmaniose.

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Section: Methodssupporting

confidence: 56%

Section: Methodsmentioning

confidence: 94%

Molecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantumin Mato Grosso do Sul, Brazil

Sousa

André

Herrera³

et al. 2013

Rev. Bras. Parasitol. Vet.

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“…[27][28][29] Among new world leishmaniasis, one report showed L. braziliensis DNA in the saliva of a patient with ATL without clinical visceral or mucosal infection. 30 Tonsils and adenoids have been identified as sites suspected of containing macrophage-engulfed Leishmania. 17,27 Although this study identified DNA of Leishmania in oral secretions, the transmission of the disease from person to person by way of the upper respiratory and alimentary tracts has to be further studied.…”

Section: Discussionmentioning

confidence: 99%

Consecutive Cutaneous and Visceral Leishmaniasis Manifestations Involving a Novel Leishmania Species in Two HIV Patients in Thailand

Chusri¹,

Hortiwakul²,

Silpapojakul³

et al. 2012

The American Journal of Tropical Medicine and Hygiene

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Abstract. Leishmaniasis is an emerging disease in Thailand. Herein, we report on two human immunodeficiency virus (HIV)-infected patients with leishmaniasis who presented with overlapping manifestations between cutaneous and visceral leishmaniasis. Sequencing analysis of the internal transcribed spacer 1 (ITS1) of the ribosomal RNA gene showed that the species was identical to a new species recently described in Thailand. The detection of DNA of this Leishmania species in saliva may have important implications for transmission and epidemiological studies.

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“…[18][19][20] Collection of saliva is noninvasive and convenient for field studies. We describe a PCR to amplify the internal transcribed spacer1 (ITS1) gene of L. siamensis from six infected patients and compare it with specimens collected from patients and different clinical presentations.…”

mentioning

confidence: 99%

Detection of Leishmania siamensis DNA in Saliva by Polymerase Chain Reaction

Phumee

Kraivichian²,

Chusri³

et al. 2013

The American Journal of Tropical Medicine and Hygiene

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Abstract. Polymerase chain reaction was used to detect Leishmania siamensis DNA from clinical samples collected from six leishmaniasis patients during 2011-2012. The samples used in this study came from bone marrow, blood, buffy coat, saliva, urine, and tissue biopsy specimens. Saliva was a good source for L. siamensis DNA by polymerase chain reaction. L. siamensis DNA was also found in saliva of an asymptomatic case-patient. Levels of L. siamensis DNA in saliva decreased until being undetectable after treatment. These levels could be used as a marker to evaluate efficacy of the treatment. A larger study is needed to evaluate this method as a screening and survey tool to study the silent background of Leishmania infection among the at-risk population.Leishmaniasis is a neglected tropical diseases caused by an obligate intracellular protozoa belonging to the genus Leishmania. The disease is transmitted to vertebrate hosts by infected female sand flies taking a blood meal.1 Leishmaniasis presents in three clinical forms; visceral, cutaneous, and mucocutaneous.2 Clinical presentation of leishmaniasis depends on the species of Leishmania and the immunity of the host. Detection and species identification of the parasites is essential for prognostic and therapeutic reasons and surveys.

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DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis

Cited by 10 publications

References 10 publications

Molecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantumin Mato Grosso do Sul, Brazil

Molecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantumin Mato Grosso do Sul, Brazil

Consecutive Cutaneous and Visceral Leishmaniasis Manifestations Involving a Novel Leishmania Species in Two HIV Patients in Thailand

Detection of Leishmania siamensis DNA in Saliva by Polymerase Chain Reaction

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