Assessment of genetic relationship between Klebsiella pneumoniae and Klebsiella oxytoca samples isolated from a dental office

Pimenta-Rodrigues, M.; Fusco-Almeida, A. M.; Bertoni, Bianca Waléria; Pietro, Rosemeire Cristina Linhari Rodrigues

doi:10.1590/s1678-91992008000400012

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…Molecular typing of the isolates was carried by random amplified polymorphic DNA (RAPD) using oligonucleotides Operon 18; 5' CAGCACCCAC 3' [ 20 ]. PCR mixture was composed of the genomic DNA (100 ng), Operon 18 primer (20 μM), 1 μl dNTP (250 μM each), 5× Flexi buffer GoTaq ® , 1 μl MgCl 2 (25mM), 1.5 U DNA polymerase FlexiTaq (Promega, USA) and H 2 O up to a final volume of 20 μl.…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization of Klebsiella pneumoniae Clinical Isolates with Elevated Resistance to Carbapenems

Barwa¹,

Shaaban²

2017

TOMICROJ

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Background:Emergence of carbapenems-resistant K. pneumoniae represents a serious challenge for antimicrobial therapy.Objective:The aim of this research is to determine different mechanisms mediating the emergence of K. pneumoniae isolates with high-level carbapenem resistance.Method:A total of 80 K. pneumoniae isolates were purified from sputum and urine specimens. The minimum inhibitory concentrations (MICs) of imipenem and meropenem were determined by broth microdilution method. Carbapenemases were detected by Modified Hodge test and PCR. Additionally, the copy numbers of the identified genes (blaVIM-1, blaNDM-1 and blaOXA-48) were quantified by RT-PCR. The outer membrane proteins OmpK35 and OmpK36 of the resistant isolates were analyzed.Results:Eight isolates were resistant to carbapenems; six of these isolates possessed elevated MICs to imipenem and meropenem (≥16 µg/ml). Carbapenem resistant isolates harbored blaNDM-1 (n=5), blaVIM-1 (n=4) and blaOXA-48 (n=1) with some isolates had multiple carbapenemases genes. Six isolates with high MICs to imipenem contained multi-copies of the carbapenemases genes along with the lack of OmpK35. Isolates with intermediate resistance to carbapenems (MIC; 4-8 µg/ml) did not exhibit multiple carbapenemases but lacked the OmpK35. Random amplified polymorphic DNA exhibited three different patterns and indicated that five isolates encoded the same pattern P1.Conclusion:This study elucidated that multiple carbapenemases genes, high copy number of carbapenemases and loss of the porin OmpK35 could collectively contribute to the emergence of K. pneumoniae isolates with high resistance to carbapenems. Hence, more restrictions should be applied on the use of carbapenems to reduce the emergence of the resistant clones.

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Section: Methodsmentioning

confidence: 99%

Molecular Characterization of Klebsiella pneumoniae Clinical Isolates with Elevated Resistance to Carbapenems

Barwa¹,

Shaaban²

2017

TOMICROJ

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Influence of biological fluids in bacterial viability on different hospital surfaces and fomites

Esteves

Pereira

Souza

et al. 2016

American Journal of Infection Control

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Antimicrobial Activity of Herbal Toothpaste against Organisms Causing Dental Caries

Raj

Ramsurn

Thomas

2020

ARRB

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Aim: Dental caries occurs as a result of acid produced by bacteria which destroys the enamel and underlying dentin. A study was carried out to isolate microorganisms that cause dental caries in patients belonging to different age groups and find out the antimicrobial activity of various toothpastes against them. Study Design: Collection of samples for isolation of caries causing organisms, to identify them using biochemical tests and test the activity of herbal toothpastes against them. Place and duration of study: Samples were collected from Krish Multispeciality Clinic and Prashanth Dental Clinic, Nagole between June’2016 – December’2017 and worked on, at Microbiology Department, St. Francis College for women, Hyderabad. Methodology: Ten samples of each age group from 10-20 yrs., 20-40 yrs. and 40-60 yrs. were isolated. The organisms were identified by carrying out various biochemical tests according to Bergey’s Manual of Systematic Bacteriology and molecular characterization based on 16s rRNA typing. Identified organisms were used to test the antimicrobial activity of herbal toothpastes by employing agar well diffusion method. ANOVA is used for statistical analysis. Results and Conclusion: Patanjali herbal products like Dantkanti are gaining lot of importance and have become a part of life of common man because of their effectivity. Our studies revealed that out of the various toothpastes tested, Patanjali toothpaste had good antimicrobial activity against all the isolates. On comparative analysis of f-ratio and P significant value (< .05.) of all pairs, it is observed that Patanjali has good antimicrobial activity against all isolates tested. The efficacy of Meswak is almost similar to that of Patanjali.

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Assessment of genetic relationship between Klebsiella pneumoniae and Klebsiella oxytoca samples isolated from a dental office

Cited by 3 publications

References 17 publications

Molecular Characterization of Klebsiella pneumoniae Clinical Isolates with Elevated Resistance to Carbapenems

Molecular Characterization of Klebsiella pneumoniae Clinical Isolates with Elevated Resistance to Carbapenems

Influence of biological fluids in bacterial viability on different hospital surfaces and fomites

Antimicrobial Activity of Herbal Toothpaste against Organisms Causing Dental Caries

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