rACLF, a recombinant snake venom metalloprotease, activates endothelial cells in vitro

Moraes, Caroline Krieger de; Fritzen, Márcio; Chudzinski-Tavassi, A.M.; Selistre-de-Araújo, Heloísa Sobreiro

doi:10.1590/s1678-91992008000100009

Cited by 4 publications

(1 citation statement)

References 35 publications

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“…These local effects have been defined as pain, hemorrhage, edema, myonecrosis and inflammation. The snake venom metalloproteinases (SVMPs) have been considered as playing a crucial role in the development of the local venom-induced pathogenesis (2)(3)(4).…”

Section: Introductionmentioning

confidence: 99%

Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study

Baldo

Ferreira²,

Lopes

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:The damaging effects of neuwiedase, a non-hemorrhagic snake venom metalloproteinase from P-I class, on gastrocnemius muscle are studied herein. Following neuwiedase injection, ultrastructural alterations were detected early showing disarrangement of skeletal muscle fibers (characterized by discontinuity of Z lines), mitochondrial swelling, and disruption of plasma membrane and basal lamina. Degradation of skeletal muscle and the appearance of an amorphous substance, primarily composed of cellular debris, were noted after 24 hours. The presence of neuwiedase at the injection site (detected by immunocytochemistry) revealed highly specific labeling of myofibril components of damaged myocytes. In addition, proteolysis of muscle proteins assayed through myofibrils extracted from gastrocnemius muscle indicated that neuwiedase provoked degradation of myofibrils, especially myosin. These results suggest that skeletal muscle damage, induced by neuwiedase, is probably due to its proteolytic action on myofibrils, which are responsible for the maintenance of the cellular architecture.

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Section: Introductionmentioning

confidence: 99%