2007
DOI: 10.1590/s1678-91992007000300009
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Abstract: Snake venom is characterized by hemorrhagic, coagulant, proteolytic and myotoxic activities which in Bothrops jaracussu venom are related to intraspecific variations. In the present study, female Swiss mice were divided into two groups: treated with 25µg or 50µg venom. These were subdivided into three groups of six animals each, according to blood collection: 2, 4 or 24h after venom injection. Animals were anesthetized using diethyl-ether inhalation and 1ml of blood was collected by heart puncture. Then, the f… Show more

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Cited by 3 publications
(1 citation statement)
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“…The animals received 25μg of B. alternatus venom (Zeni et al, 2007) diluted in 0.25mL of PBS intra-dermal (ID) on the right femoral biceps, using a hypodermic syringe, and they were randomly distributed into three equal groups (n= 4): G1, G2 and G3. Thirty minutes after the challenge (venom inoculation), the animals were treated as follows: G1 received 0.25mL of PBS ID; G2 received 25mg of EDTA diluted in 0.25mL of PBS intramuscularly (IM) in the right femoral biceps; and G3 received the same volume and dose of EDTA intravenously (IV) by puncture of the right ear lateral vein, followed by a bolus of 1mL of 0.9% NaCl.…”
Section: Methodsmentioning
confidence: 99%
“…The animals received 25μg of B. alternatus venom (Zeni et al, 2007) diluted in 0.25mL of PBS intra-dermal (ID) on the right femoral biceps, using a hypodermic syringe, and they were randomly distributed into three equal groups (n= 4): G1, G2 and G3. Thirty minutes after the challenge (venom inoculation), the animals were treated as follows: G1 received 0.25mL of PBS ID; G2 received 25mg of EDTA diluted in 0.25mL of PBS intramuscularly (IM) in the right femoral biceps; and G3 received the same volume and dose of EDTA intravenously (IV) by puncture of the right ear lateral vein, followed by a bolus of 1mL of 0.9% NaCl.…”
Section: Methodsmentioning
confidence: 99%