Interleukin-15: its role in microbial infections

Bannwart, Camila Ferreira; Nakaira, Erika Takahashi; Sartori, Arthur Alves; Peraçoli, Maria Terezinha Serrão

doi:10.1590/s1678-91992007000300002

Cited by 4 publications

(3 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All cytokines mentioned lead to the generation of NK cells with functional "memory" properties (Zhuang et al, 2018). It has been shown that the cell line of monocytes and macrophages can respond to the encouragement of IL-15, increasing Il-18 and IL-12 (Bannwart et al, 2007). Furthermore, the IL-18 is a cytokine responsible for the beginning and progression of the periodontal disease (Figueredo et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Immunological evaluation of the crevicular fluid in patients with gingivitis, periodontitis, and peri-implantitis: a 1-year cross-sectional study

Faria

Furtado

Assunção

et al. 2021

RSD

View full text Add to dashboard Cite

Objective: To assess the levels of IL-12, IL-15, IL-18, and IL-32 in the gingival crevicular fluid (GCF) and peri-implant crevicular fluid (PICF) in patients with gingivitis, periodontitis, and peri-implantitis before and one year after implant installation. Material and Methods: Forty-nine samples of GCF and PICF were collected from March 2018 to March 2019. The patients were classified: patients with gingivitis (n=7), patients with periodontitis (n=14), patients with peri-implantitis (n=4) and healthy patients (n=24). The crevicular fluid from the 49 patients was collected before implant installation (n=8) and one year after implant placement (n=8). The Enzyme-Linked Immunosorbent Assay (ELISA) was used to evaluate the levels of cytokines in crevicular fluid. Results: Patients with gingivitis, periodontitis, and peri-implantitis showed higher concentrations of IL-12, IL-15, IL-18, and IL-32 when compared with control group. In general, the levels of IL-12 and IL-15 increased when compared to the moments before and after implant installation. There was also an increase in the concentration of IL-18 in the control group volunteers after implant installation. Conclusion: The results and methodology of this study showed that there was no difference in the synthesis of IL-12, IL-15, IL-18, and IL-32 in healthy individuals or in those with periodontal injuries. However, there was an increase in the cytokines IL-12, IL-15, and IL-18 one year after implant installation, which would be increasing the inflammatory activity in peri-implantitis.

show abstract

Section: Discussionmentioning

confidence: 99%

Immunological evaluation of the crevicular fluid in patients with gingivitis, periodontitis, and peri-implantitis: a 1-year cross-sectional study

Faria

Furtado

Assunção

et al. 2021

RSD

View full text Add to dashboard Cite

show abstract

“…The literature documents the role of IL-15 in overcoming the above-mentioned challenges (3). IL-15 as vaccine adjuvant enhances the protective immunity against infectious pathogens, including influenza virus, Toxoplasma gondii, Herpes simplex virus, Trypanosoma cruzi, and complex intracellular protozoan pathogen by boosting the survival and improving cytotoxic activity of T and NK cells as well as IFN-g production (4)(5)(6)(7)(8)(9). Co-administration of plasmid expressing IL-15 along with HIV-DNA vaccine has shown to generate the higher frequency of CD8 + Tem cells (10).…”

Section: Introductionmentioning

confidence: 99%

Development of Stable Chimeric IL-15 for Trans-Presentation by the Antigen Presenting Cells

2021

View full text Add to dashboard Cite

IL-15 is one of the important biologics considered for vaccine adjuvant and treatment of cancer. However, a short half-life and poor bioavailability limit its therapeutic potential. Herein, we have structured IL-15 into a chimeric protein to improve its half-life enabling greater bioavailability for longer periods. We have covalently linked IL-15 with IgG2 base to make the IL-15 a stable chimeric protein, which also increased its serum half-life by 40 fold. The dimeric structure of this kind of IgG based biologics has greater stability, resistance to proteolytic cleavage, and less frequent dosing schedule with minimum dosage for achieving the desired response compared to that of their monomeric forms. The structured chimeric IL-15 naturally forms a dimer, and retains its affinity for binding to its receptor, IL-15Rβ. Moreover, with the focused action of the structured chimeric IL-15, antigen-presenting cells (APC) would transpresent chimeric IL-15 along with antigen to the T cell, that will help the generation of quantitatively and qualitatively better antigen-specific memory T cells. In vitro and in vivo studies demonstrate the biological activity of chimeric IL-15 with respect to its ability to induce IL-15 signaling and modulating CD8+ T cell response in favor of memory generation. Thus, a longer half-life, dimeric nature, and anticipated focused transpresentation by APCs to the T cells will make chimeric IL-15 a super-agonist for memory CD8+ T cell responses.

show abstract

“…Moreover, IL‐15 may directly activate APCs to secrete IL‐12, which in turn induces IFN‐γ for further activation of dendritic cells and macrophages and co‐stimulation of CD8+ T cells (17). It has also been documented that IL‐15 induces proliferation and migration of the activated or memory lymphocytes involved in the host defense to microbial infection (18). To find the role of IL‐15 in the immunity against Brucella in the mice immunized with DNA vaccine containing Brucella antigen with and without IL‐15, Cai and colleagues showed that this cytokine acts as an adjuvant which induces protection to this organism (19).…”

Section: Introductionmentioning

confidence: 99%

Association of interleukin‐15 single nucleotide polymorphisms with resistance to brucellosis among Iranian patients

et al. 2011

View full text Add to dashboard Cite

Interleukin (IL)-15, a Th1-related cytokine, triggers inflammatory cells' recruitment and increases the expression of interferon gamma (IFN-γ), which is an important cytokine in the immunity against brucellosis. Different single-nucleotide polymorphisms (SNPs) have been observed in the IL-15 gene, so this study aimed to investigate the probable association between these SNPs and susceptibility to brucellosis among Iranian patients. A total of 190 patients with brucellosis and 83 healthy milk farmers who consumed contaminated raw milk and dairy products from animals involved with brucellosis were included in this study. All the patients and the controls were genotyped for four IL-15 polymorphisms at positions 267, 367, 13687 and 14035 using polymerase chain reaction-restriction fragment length polymorphisms. The 267C and 13687A alleles, haplotypes CGCT and CAAA and the 267CC and 13687AA genotypes were significantly more frequent in the controls than in the patients (P = 0.014, 0.03, 0.006, 0.024, 0.026 and 0.01, respectively), so the variation in the IL-15 gene may be one of the factors affecting the resistance to brucellosis. In contrast, the frequency of haplotypes CGCA and TACT was significantly higher in patients compared with controls (P = 0.015 and 0.007, respectively), and interestingly the last one was observed only in the patients; therefore, it may serve as a predictive factor for brucellosis. In conclusion, it could be suggested that IL-15 genetic variants can affect resistance or susceptibility to human brucellosis among Iranian patients.

show abstract

Interleukin-15: its role in microbial infections

Cited by 4 publications

References 38 publications

Immunological evaluation of the crevicular fluid in patients with gingivitis, periodontitis, and peri-implantitis: a 1-year cross-sectional study

Immunological evaluation of the crevicular fluid in patients with gingivitis, periodontitis, and peri-implantitis: a 1-year cross-sectional study

Development of Stable Chimeric IL-15 for Trans-Presentation by the Antigen Presenting Cells

Association of interleukin‐15 single nucleotide polymorphisms with resistance to brucellosis among Iranian patients

Contact Info

Product

Resources

About