Intraspecific variation in protein pattern of red scorpion (Mesobuthus tamulus, coconsis, pocock) venoms from Western and Southern India

Badhe, Ravindra V.; Thomas, Asha; Harer, S. L.; Deshpande, A. D.; Salvi, Nitin; Waghmare, A.

doi:10.1590/s1678-91992006000400008

Cited by 16 publications

(11 citation statements)

References 7 publications

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“…This observation can be explained by genetic factors since the insects belong to different families. This result has been observed previously in works concerning the influence of the environmental component on venom chemical composition (Tsai et al, 2004;Badhe et al, 2006;Abdel-Rahman et al, 2011;Cologna et al, 2013). Studies with social Hymenoptera have also shown that genetic factors and age can be responsible for the venom chemical composition and some compounds are characteristic of particular species (Ferreira-Junior et al, 2010;Cologna et al, 2013).…”

Section: Discussionsupporting

confidence: 82%

“…However, venom is a complex mixture of substances and the variation in its chemical composition cannot be attributed to any single factor. Studies have found that in addition to genetic factors, environmental variables, such as geographic location and climate, can also affect the distribution of food and therefore influence the diets of the organisms (Tsai et al, 2004;Badhe et al, 2006;Abdel-Rahman et al, 2009, 2011Cologna et al, 2013). Hence, environmental factors also exert an influence on venom chemical composition.…”

Section: Discussionmentioning

confidence: 99%

“…It has been found that variation in venom chemical composition among organisms is related to their geographic position, genetic, environmental conditions, sex, age, diet and also depends on seasonal factors (Daltry et al, 1996;Badhe, 2006;Abdel-Rahman et al, 2009, 2011.…”

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confidence: 99%

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Evaluation of Inter and Intraspecific Differences in the Venom Chemical Compositions of Polybia paulista Wasps and Ectatomma brunneum Ants Using FTIR-PAS

et al. 2019

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Wasps can synthesize chemical compounds called venom whose function is to overcome prey and assist in defense of the colonies. Geographic Parameters such as sex, age, the season of the year, and diet determined the composition of the venom location, genetics, environment. However, studies on the compositional variability of venom are still limited due to the difficulty in obtaining samples and the complexity of these substances. This work describes the use of the Fourier Transform Infrared Photoacoustic Spectroscopy (FTIR-PAS) to investigate inter- and intraspecific variability in the venom chemical composition (VCC) of the social wasp Polybia paulista (Von Ihering 1896) and the ant Ectatomma brunneum (Smith 1858). The results reveal significant differences in VCC among the ant and wasp, even for samples obtained from the same environment. The genetic component, therefore, seemed to be the predominant factor determining the compounds present. The findings also showed that exogenous factors, such as diet, could also be responsible for intraspecific differences, especially in wasps. The FTIR-PAS technique proved to be a reliable way of assessing intra- and interspecific differences in social Hymenoptera VCC.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

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Evaluation of Inter and Intraspecific Differences in the Venom Chemical Compositions of Polybia paulista Wasps and Ectatomma brunneum Ants Using FTIR-PAS

et al. 2019

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“…These differences may have clinical ramifications as it has been reported that within the state of Maharashtra, fatal Toxinology DOI 10.1007/978-94-007-6647-1_5-1 # Springer Science+Business Media Dordrecht 2014 human envenomations only occur within the Konkan region (Bawaskar and Bawaskar 1999). Intraspecific variations have also been seen using SDS-PAGE, between the venoms of scorpions collected in Western India (Ratnagiri, Chiplun, and Ahmednagar), as distinct from those collected in Chennai, in the south of the country (Badhe et al 2006). The same authors have detected differences in the blood sodium levels of mice injected with these differently sourced venoms (Badhe et al 2007).…”

Section: Venom Constituentsmentioning

confidence: 99%

Scorpion Venom Research Around the World: Indian Red Scorpion

Strong

Mukherjee²,

Shah³

et al. 2014

Toxinology

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The Indian red scorpion is the medically most important scorpion on the Indian subcontinent, and a sting is often lethal without treatment, especially to young children. The correct identification of this species is Hottentotta tamulus; the more commonly known names, Buthus tamulus and Mesobuthus tamulus, and the suggestions of subspecies (Pocock) are incorrect and should be discouraged. Mild cases of H. tamulus envenoming show vasoconstriction and hypertension resulting from a massive release of catecholamines. Severe cases result in hypotension, pulmonary edema and myocardial dysfunction. Animal studies indicate that catecholamine release is as a consequence of prolonged sodium channel activation and potassium channel inhibition. Pulmonary edema results from many subcellular events, including kinin activation and stimulation of central serotonin receptor subtypes. Treatment with a specific antibody (with or without the a-adrenoreceptor blocker, prazosin, dependent on symptoms and clinical severity) is presently the preferred therapy. The few venom constituents that have been characterized in detail include iberiotoxin, a specific blocker of high conductance, calcium-activated potassium channels; tamapin, a specific blocker of low conductance calcium activated potassium channels (K Ca 2.2 subtype); and the short peptide insectotoxins, ButaIT and BtlTx3.

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“…[20] detected intraspecific protein diversity using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in venom from the red scorpion Mesobuthus tamulus from western and southern India. The authors suggested that differences in the band patterns of separated proteins in all venom samples indicated the existence of genetic variation in venom production among scorpion strains of western and southern India.…”

Section: Introductionmentioning

confidence: 99%

The Cuban scorpion Rhopalurus junceus (Scorpiones, Buthidae): component variations in venom samples collected in different geographical areas

Rodríguez-Ravelo¹,

Coronas

Zamudio

et al. 2013

Journal of Venomous Animals and Toxins including Tropical Disea

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BackgoundThe venom of the Cuban scorpion Rhopalurus junceus is poorly study from the point of view of their components at molecular level and the functions associated. The purpose of this article was to conduct a proteomic analysis of venom components from scorpions collected in different geographical areas of the country.ResultsVenom from the blue scorpion, as it is called, was collected separately from specimens of five distinct Cuban towns (Moa, La Poa, Limonar, El Chote and Farallones) of the Nipe-Sagua-Baracoa mountain massif and fractionated by high performance liquid chromatography (HPLC); the molecular masses of each fraction were ascertained by mass spectrometry analysis. At least 153 different molecular mass components were identified among the five samples analyzed. Molecular masses varied from 466 to 19755 Da. Scorpion HPLC profiles differed among these different geographical locations and the predominant molecular masses of their components. The most evident differences are in the relative concentration of the venom components. The most abundant components presented molecular weights around 4 kDa, known to be K+-channel specific peptides, and 7 kDa, known to be Na+-channel specific peptides, but with small molecular weight differences. Approximately 30 peptides found in venom samples from the different geographical areas are identical, supporting the idea that they all probably belong to the same species, with some interpopulational variations. Differences were also found in the presence of phospholipase, found in venoms from the Poa area (molecular weights on the order of 14 to 19 kDa). The only ubiquitous enzyme identified in the venoms from all five localities studied (hyaluronidase) presented the same 45 kD molecular mass, identified by gel electrophoresis analysis.ConclusionsThe venom of these scorpions from different geographical areas seem to be similar, and are rich in peptides that have of the same molecular masses of the peptides purified from other scorpions that affect ion-channel functions.

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Intraspecific variation in protein pattern of red scorpion (Mesobuthus tamulus, coconsis, pocock) venoms from Western and Southern India

Cited by 16 publications

References 7 publications

Evaluation of Inter and Intraspecific Differences in the Venom Chemical Compositions of Polybia paulista Wasps and Ectatomma brunneum Ants Using FTIR-PAS

Evaluation of Inter and Intraspecific Differences in the Venom Chemical Compositions of Polybia paulista Wasps and Ectatomma brunneum Ants Using FTIR-PAS

Scorpion Venom Research Around the World: Indian Red Scorpion

The Cuban scorpion Rhopalurus junceus (Scorpiones, Buthidae): component variations in venom samples collected in different geographical areas

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