Abstract:after 4h of monocyte infection with the fungus. TNF-α production by these cells was determined in supernatant cultures by enzyme immunoassay (ELISA), and fungicidal activity of monocytes against Pb18 was assessed by viable fungi recovery from 4h co-culture in Blood Heart Infusion-Agar (BHI-Agar) and counting of colonyforming units after 10 days. The results showed that monocyte incubation with TGFβ 1 concentrations (31.2 pg/ml to 500 pg/ml) suppressed H 2 O 2 release in a dosedependent manner. The Pb18 infecti… Show more
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