Clinical and morphological evaluation of snake venom derived fibrin glue on the tendon healing in dogs

Abstract

The aim of this study was to evaluate the effect of snake venom derived fibrin glue on the healing of the deep digital flexor tendon, during three periods. The tendon of the 2nd digit of 30 thoracic limbs of dogs was partially sectioned for glue application. Biopsies were performed 7, 15, and 30 days post surgery for the clinical and morphological study of tendons. Analysis of the results showed that 73.3% of the tendons showed stump retraction and 16.6% moderate to excessive adherence, which affected sliding. There was a significant difference in the number of inflammatory cells among the three studied periods, being the highest on day 15. The morphological analysis revealed a typical tendon healing process with a lower level of inflammation in the acute phase, facilitating the cicatricial maturation phase. Snake venom derived fibrin glue promotes the healing in dog flexor tendon

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review

“…The thickness of healing tissue was higher in G2 than G1 and G3.The experimental model was appropriate to achieve the proposed objectives. Fibrin sealant produced less inflammation in the exudative phase and eased the evolution of proliferation and maturation phases.The amount of fibrin sealant used in surgery was not reported. Ferraro et al (2005) [45] Assessment of the effect of the fibrin sealant derived from snake venom on the tendon force in the healing process in dogs.Left and right thoracic limbs of 15 dogs were used. Members were divided into three groups (n = 10).…”

“…Ferraro et al [45,64], evaluated the healing strength of the tendon of the thoracic limbs of dogs and their clinical evolution, using fibrin sealant as a substitute for tenorrhaphy. The assessment of four biomechanical properties (resilience, rigidity, maximum limit and stability limit) allowed observing that tendon healing achieved progressive resistance with maximum tensile strength time.…”

Reparo ósseo de enxerto autógeno estabilizado com adesivo de fibrina: análise histológica e histomorfométrica

“…A eficácia deste novo selante de fibrina é relatada na literatura, havendo de resultados satisfatórios quando utilizado em animais visando reparos nervosos (IUAN et al, 1995;FRANCIOSI;PALHARES, 1995;CHALHOUB et al 2000;SILVA et al, 2012;VITERBO et al, 2012) e reparos teciduais (RAHAL et al, 2004;FERRARO et al, 2005;SAMPAIO et al, 2007). BARANAUSKAS; da CRUZ-HOFLING et al, 2004;KAZEM SHAKOURI et al, 2010).…”