Effect of snake venom derived fibrin glue on the tendon healing in dogs: clinical and biomechanical study

Abstract

The aim of this study was to evaluate the effect of snake venom derived fibrin glue on the strength oftendon healing in dogs. The deep digital flexor tendon of the 5th digit of 24 thoracic limbs was partially sectioned for adhesive application. On the 7th, 15th, and 30th postoperative day tendons segments were removed for the clinical and biomechanical study. Results indicated that 62.5% of the tendons showed stump retraction and 20.8% moderate to excessive adherence, which affected gliding. The biomechanical evaluation showed that, over time, tendon healing gained progressive resistance for maximum traction and permanent deformations with satisfactory results on the 15th day for rigidity and resilience compared to the other two studied periods. Snake venom derived fibrin glue promotes healing in dog flexor tendon

Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells

“…In the biomechanical analysis, no differences were observed between the transected groups for the stress parameter on the 21st day, supporting no differences in the amount of collagen type I in the entire tendon, which is directly related to the resistance of tendon. Although, the literature points to the beneficial effects of FS and ASC on the biomechanics of tendons in the healing process [83]. Therefore, it is possible to conclude that the structural data did not directly reflect the biomechanics of the tendons, perhaps due to the recovery time, 21 days, which is marked by the beginning of the remodeling process.…”

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review

“…Ferraro et al [45,64], evaluated the healing strength of the tendon of the thoracic limbs of dogs and their clinical evolution, using fibrin sealant as a substitute for tenorrhaphy. The assessment of four biomechanical properties (resilience, rigidity, maximum limit and stability limit) allowed observing that tendon healing achieved progressive resistance with maximum tensile strength time.…”

“…The assessment of four biomechanical properties (resilience, rigidity, maximum limit and stability limit) allowed observing that tendon healing achieved progressive resistance with maximum tensile strength time. Thus, fibrin sealant derived from snake venom obtained positive results, promoting healing in the flexor tendon of dogs [64].…”

A new heterologous fibrin sealant as scaffold to recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins for the repair of tibial bone defects

“…Fibrin is a substance with adhesive properties whose application has been studied in different tissues, including the tendon of dogs (Ferraro et al, 2005). Also, its adhesive properties have been proven to be biocompatible when associated with mesenchymal stem cells derived from Wistar rats (MSCs) (Gasparotto et al, 2014).…”