2004
DOI: 10.1590/s1678-91992004000300004
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Abstract: ABSTRACT. We investigated the in vitro process of cell death caused by Egyptian cobra venom on primary human embryonic kidney (293T) and mouse myoblast (C2C12) cell lines. The aim of these studies was to provide further information about triggering cell death, and suggest methods for eliminating unwanted cells, such as tumour cells. Both cell lines were treated with 10, 20, and 50 µg/ml of Egyptian cobra (Naja haje) venom in serum free media (SFM) and incubated for 8 hours. Total activities of the lactate dehy… Show more

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Cited by 16 publications
(15 citation statements)
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“…Our interpretation of the results suggests that the LD 50 value is approximately ten times greater than the IC 50 value for crude venom from Macrovipera lebetina lebetina. Our results agree with those from other authors (40)(41)(42). Another important point is that the venom enhances the cytotoxic response of L929 cells when administered for longer periods (48 hours) with an IC 50 value of 0.62 ± 0.18 µg/ mL.…”
Section: Discussionsupporting
confidence: 93%
See 1 more Smart Citation
“…Our interpretation of the results suggests that the LD 50 value is approximately ten times greater than the IC 50 value for crude venom from Macrovipera lebetina lebetina. Our results agree with those from other authors (40)(41)(42). Another important point is that the venom enhances the cytotoxic response of L929 cells when administered for longer periods (48 hours) with an IC 50 value of 0.62 ± 0.18 µg/ mL.…”
Section: Discussionsupporting
confidence: 93%
“…Currently, researchers suggest that cell-based assay to examine venom cytotoxicity is an alternative to animal testing (18,19,(40)(41)(42). Our in vitro results show that crude venom from Macrovipera lebetina lebetina is highly cytotoxic for cultured fibroblasts causing decreased viability, the disappearance of normal morphological characteristics, rounding up, detachment and death at the highest concentration.…”
Section: Discussionmentioning
confidence: 78%
“…), as well as localised pain, swelling and tissue necrosis (Joubert and Talfaard ; Omran et al . ; Simpson and Jacobsen ). N. h. haje is a large, but rather slender, snake with a short tail and is mainly crepuscular (Baha El Din ).…”
Section: Introductionmentioning
confidence: 98%
“…In Egypt, N. haje haje lives in habitats along the Nile Valley, western desert, and the Mediterranean coastal desert, particularly in cultivated land and old buildings. Like all cobras, its venom is primarily neurotoxic, causing respiratory failure (El-Kady et al 2009), as well as localised pain, swelling and tissue necrosis (Joubert and Talfaard 1978;Omran et al 2004;Simpson and Jacobsen 2009). N. h. haje is a large, but rather slender, snake with a short tail and is mainly crepuscular (Baha El Din 2006).…”
Section: Introductionmentioning
confidence: 99%
“…Although in vivo experiments are adequate techniques to investigate the action of snake venoms on the overall muscle tissue scenario, these models preclude the analysis of some specific aspects of the toxin action on muscle cells. In that sense, as an approach to the characterization of the mechanisms involved in myotoxicity, cultured cells (11,12) or isolated enzymes -such as myotoxic PLA 2 s -(13-17) have been evaluated as targets for crude venoms.…”
Section: Introductionmentioning
confidence: 99%