Effect of fibrin glue derived from snake venom on the viability of autogenous split-thickness skin graft

Abstract

The aim of this study was to analyze the effect of snake venom derived from fibrin glue on the viability of split-thickness skin graft. Nine crossbreed dogs were used. Full-thickness skin segments measuring 4 x 4 cm were bilaterally excised from the proximal radial area on each dog. A split-thickness skin graft was harvestedfrom left lateral thoracic area using a freehand graft knife, and was secured to the left recipient bed using several simple interrupted sutures of 3-0 nylon (sutured graft). A split-thickness skin graft was harvested from the right lateral thoracic area using a graft knife. Fibrin glue derived from snake venom was applied to the recipient bed, and 8 equidistant simple interrupted sutures of 3-0 nylon were used to secure the skin graft (glued graft). Viable and nonviable areas were traced on a transparent sheet and measured using a Nikon Photomicroscope connected to a KS-300 image analysis system. The skin graft and recipient bed were collected from three dogs at day 7, 15, and 30 postoperative. The glued grafts had statistically higher graft viability than sutured grafts. Histological examination showed that the tissue repair process in the glued grafts was more accentuated than sutured grafts. It was possible to conclude that fibrin glue derived from snake venom increased survival of autogenous split-thickness skin graft

A new heterologous fibrin sealant as scaffold to recombinant human bone morphogenetic protein-2 (rhBMP-2) and natural latex proteins for the repair of tibial bone defects

“…Factor XIII, when activated by thrombin in the presence of calcium (Ca 2+ ), reacts with the fibrin polymers, forming a stable and insoluble clot (Martinowitz and Spotnitz, 1997). These glues are used for tissue regeneration procedures in different areas of medicine as hemostatic agents, healing inducers, cavity sealants, and drug-delivery vehicles (Borst et al, 1982;Kram et al, 1989;Alving et al, 1995;Carvalho et al, 1997;Sartori Filho et al, 1998;Leite et al, 2000;Rahal et al, 2004;Dal Pizzol et al, 2009). However, the role of fibrin sealants in the healing of bone fractures and osteogenesis is not fully understood, with some studies recommending its application, whereas others do not (Abiraman et al, 2002).…”

“…In the present study, histomorphometric analysis revealed a greater relative volume of young bone in animals receiving this sealant. Therefore, the use of this new sealant becomes an interesting alternative for clinical and surgical applications since its does not contain human blood and permits custom formulation according to clotting time (Rahal et al, 2004;Barros et al, 2009). The new sealant was evaluated in cell culture to determine its suitability as a scaffold for bone marrow-derived MSCs.…”

Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

“…In addition, patients may develop antibodies against bovine thrombin 13 . In an attempt to overcome these difficulties, another sealant consisting of a thrombin-like enzyme extracted from snake venom and fibrinogen obtained from large animals to replace human components has shown excellent results in experimental studies on the regeneration of different tissues [14][15][16][17][18] , in mesenchymal cell cultures 19 , and in the healing of skin wound, venous ulcers and gingival graft sutures in humans 20,21 . This new sealant is a biodegradable product that does not cause adverse reactions, does not contain human blood components, has good adhesive capacity, and does not transmit infectious diseases 22 .…”

Efeito de cianoacrilatos e adesivo tissular a base de veneno de cobra na citotoxicidade sobre fibroblastos gengivais e estresse oxidativo sobre células do biofilme de <i>C. albicans</i>

“…O VC já foi avaliado, também, como adesivo biológico em enxerto de pele em cachorros e humanos e foi observada uma maior viabilidade e reparação tecidual além de uma excelente resultado cosmético (STOLF, 1999;RAHAL et al, 2004). Não foi observada nenhuma indicação de toxicidade local ou sistêmica (STOLF, 1999  Avaliar o nível de estresse oxidativo causado pelos produtos experimentais em células de biofilme de C. albicans.…”