Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation

Abstract

ObjectivesThe purpose of this study was to evaluate the antimicrobial activity of an acrylic resin combined with an antimicrobial polymer poly (2-tert-butylaminoethyl) methacrylate (PTBAEMA) to inhibit Staphylococcus aureus, Streptococcus mutans and Candida albicans biofilm formation. Material and MethodsDiscs of a heat-polymerized acrylic resin were produced and divided according to PTBAEMA concentration: 0 (control), 10 and 25%. The specimens were inoculated (107 CFU/mL) and incubated at 37ºC for 48 h. After incubation, the wells were washed and each specimen was sonicated for 20 min. Replicate aliquots of resultant suspensions were plated at dilutions at 37ºC for 48 h. The number of colony-forming units (CFU) was counted and expressed as log (CFU+1)/mL and analyzed statistically with α=.05. ResultsThe results showed that 25% PTBAEMA completely inhibited S. aureus and S. mutans biofilm formation. A significant reduction of log (CFU+1)/mL in count of S. aureus (control: 7.9±0.8A; 10%: 3.8±3.3B) and S. mutans (control: 7.5±0.7A; 10%: 5.1±2.7B) was observed for the group containing 10% PTBAEMA (Mann-Whitney, p<0.05). For C. albicans, differences were not significant among the groups (control: 6.6±0.2A; 10%: 6.6±0.4A; 25%: 6.4±0.1A), (Kruskal-Wallis, p>0.05, P=0.079). ConclusionsAcrylic resin combined with 10 and 25% of PTBAEMA showed significant antimicrobial activity against S. aureus and S. mutans biofilm, but it was inactive against the C. albicans biofilm.

Color and gloss evaluation of titanium dioxide coating for acrylic resin denture base

“…However, acrylic resin degrades because of bacteria and fungi adhesion onto it [2,3], microbial invasion and colonization owing to water absorption [4], and surface roughening owing to mechanical and chemical cleaning [5]. Therefore, to improve the quality of the surface of resin, the titanium dioxide coating is used .…”

“…We have reported that the use of a primer agent, which primarily consists of acryloxypropyl methoxysilane, for pre-treatment of the titanium dioxide coating affords durability of the titanium dioxide coating against brushing [8]. Moreover, the biocompatibility of a primer agent and TiO 2 coating agent has been already demonstrated [9].…”

Antimicrobial Activity of a Tissue Conditioner Combined with a Biocide Polymer

“…2,3 Several studies have investigated the addition of antimicrobial agents into denture material to reduce or to avoid microorganism development on the surface of the prostheses material. [4][5][6][7][8] Others have added antimicrobial agents to tissue conditioners. 9,10 Poly (2 tert-butylaminoethyl) methacrylate (PTBAEMA) is a polycationic polymer that acts as a substi tute for low molecular weight biocides.…”

“…The presence of amino groups caused the disorganization of the bacterial membrane and eventually, its death. 6,7 Thus, tissue conditioners could possibly exhibit activity against oral microorganisms after the incorporation of PTBAEMA into the material. The literature reveals that polymers functionalized with the association between pendent amino groups and high antimicrobial activities.…”

“…The presence of amino group caused the disorganization of the bacterial membrane and, eventually, its death. 6,7,12 The aim of the present study was to assess the effect of a tissue conditioner material, combined with the antimicrobial polymer poly (2-tert-butilaminoethyl) methacrylate (PTBAEMA), on antimicrobial activity, roughness and wettability surface. The null hypothesis tested was that the incorporation of PTBAEMA would have no effect on the roughness or wettability and would inhibit the formation of S. aureus, S. mutans and C. albicans biofilm.…”