Histopathological analysis of corticosteroid-antibiotic preparation and propolis paste formulation as intracanal medication after pulpectomy: an in vivo study

Abstract

Intracanal medication in pulpectomy therapy is used between appointments with the objective of reducing pain and inflammatory processes in pulp and periapical tissues. Propolis has been known as a natural antibiotic and has been subject of medical and dental research due to its therapeutic properties such as antibiotic, analgesic and anti-inflammatory effects. ObjectiveThe aim was to carry out an in vivo evaluation of the periapical tissue response to propolis paste when used as an intracanal medication in the teeth of dogs after pulpectomy. Material and Methods72 dog's incisors were selected for the experiment. After biomechanical preparation the root canal was filled with a corticosteroid-antibiotic preparation, experimental propolis paste, non-medicament (negative control) or non-pulpectomy at all (positive control). The medications were left inside the root canal for 7, 14 or 28 days. At the end of the experimental period histological sections were prepared and all laboratories processes for Harris hematoxylin and eosin staining was proceeded followed by the analysis using an optical microscope. Sections were classified according to a score representing the inflammatory events observed: the presence of polymorphonuclear neutrophils, polymorphonuclear eosinophils, lymphocytes and plasma cells, macrophages and/or giant cells, fibrous condensation and abscesses. ResultsThere were statistically significant differences between the tissue reactions caused by the two substances being tested, after different experimental periods, with the periapical tissue that was in contact with propolis paste exhibiting fewer inflammatory reactions in comparison to corticosteroid-antibiotic preparation. ConclusionsThe low tissue responses from propolis paste suggest that this material could be considered as an option for root canal medication after pulpectomy.

Antibacterial action of red and green propolis extract in infected root canal

“…Propolis has been studied due several properties, as antimicrobial, anti-inflammatory, antioxidant, and others [11][12][13][14][15][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33]. The chemical compounds found in propolis showed antimicrobial and tissue tolerance characteristics.…”

“…In this regard, propolis has been investigated for presenting antimicrobial properties, anti-inflammatory, antioxidant, and others [11][12][13][14][15][16][17][18][19][20][21][22][23][24]. Because of its low toxicity, it has been studied as possible intracanal medication [11,12].…”

Effects of calcium hydroxide and propolis intracanal medicaments on bond strength of resin-based endodontic sealer as assessed by push-out test

“…Propolis contains flavonoids, which are considered as its main biologically active component responsible for a large proportion of its known therapeutic properties 8) . Propolis has been proven to have antimicrobial activity against Streptococcus mutans and polymicrobial cultures collected from necrotic root canals 9) . It was also shown to be useful as a root canal dressing because of its low toxicity and broad antibacterial spectrum 10) .…”

Atividade anti-inflamatória de produtos naturais em odontologia: uma revisão sistemática

“…Os produtos medicinais, tais como própolis, babosa, camomila, guaco, quebra-pedra, boldo, gengibre apresentam diversos benefícios no processo inflamatório como aumento na produção de IL-4 e IL-10, aumento das citocinas anti-inflamatórias (LIBÉRIO et al, 2011), diminuição de exsudato inflamatório (SILVA, ALMEIDA e SOUZA, 2004), atuação como medicação intracanal após pulpectomia (RAMOS et al, 2012), prevenção da doença periodontal (KOO et al, 2000) e ativação de macrófagos (BUFALO et al, 2013).…”