Vinegar as an antimicrobial agent for control of Candida spp. in complete denture wearers

Abstract

The use of denture is known to increase the carriage of Candida in healthy patients, and the proliferation of Candida albicans strains can be associated with denture-induced stomatitis. The aim of this study was to evaluate the use of vinegar as an antimicrobial agent for control of Candida spp. in complete upper denture wearers. Fifty-five patients were submitted to a detailed clinical interview and oral clinical examination, and were instructed to keep their dentures immersed in a 10% vinegar solution (pH less than 3) overnight for 45 days. Before and after the experimental period, saliva samples were collected for detection of Candida, counting of cfu/mL and identification of species by phenotypical tests (germ tube formation, chlamidoconidia production, and carbohydrate fermentation and assimilation). The results were analyzed using Spearman's correlation and Student's t-test (p≤0.05). Candida yeasts were present in 87.3% of saliva samples before the treatment. A significant reduction was verified in CFU/mL counts of Candida after treatment. A positive correlation between Candida and denture stomatitis was verified, since the decrease of cfu/mL counts was correlated with a reduction in cases of denture stomatitis. Although it was not able to eliminate C. albicans, the immersion of the complete denture in 10% vinegar solution, during the night, reduced the amounts (cfu/mL) of Candida spp. in the saliva and the presence of denture stomatitis in the studied patients.

Effect of repeated cycles of chemical disinfection in microhardness of acrylic resins of complete denture base

“…Machado et al 12 also report that differences in the composition of the materials assessed may have been partially responsible for such findings. Pinto et al 7 observed a decreased hardness of resin Kooliner when immersed in 4% chlorhexidine digluconate and of resin Jet and Tokuyama Rebase II when immersed in 5.25% sodium hypochlorite, attributing such result to the plastifying action of disinfecting solutions, thus suggesting a change in the chemical structure of such resins when exposed to repeated disinfection cycles.…”

“…Pinto et al 7 noticed a decreased hardness of resins Jet, Kooliner and Tokuyama Rebase II when immersed in water, attributing such result to water saturation of resins, resulting in softening of the surface of such selfpolymerized resins, thus decreasing their hardness values. Such result diverges from the findings of this study, which showed increased hardness of resin Bosworth immersed in water.…”

“…Among hygiene protocols we can say that mechanical cleaning is an effective method that keeps the mucosa membrane and denture clean [6][7][8] , as well as chemical hygienization, which uses solutions such as sodium hypochlorite, alkaline glutaraldehyde, aqueous formaldehyde, chlorhexidine digluconate solution, enzymatic solutions and sodium perborate solutions. An effective alternative method that has been currently used is microwave irradiation to fight several diseases and eliminate colonization by Candida albicans 4,[8][9][10][11][12] .…”

Palatal Health of Those Wearing Complete Dentures: For How Long Must a Tissue Conditioner and Chlorhexidine Gluconate Mouthrinse be Used?

“…Denture stomatitis is a term used to describe certain pathologic changes found in the oral mucosa, and two-thirds of the elderly population wearing complete dentures have problems with their palatal and gingival mucosae (1)(2)(3)(4)(5)(6)(7)(8)(9)(10). It usually affects the hard palate and occasionally causes angular cheilitis (2,6,11).…”

Effect of affordable disinfectant solutions on Candida albicans adhered to acrylic resin for dental prosthesis

“…Despite the advantages and market availability of chemical agent's solutions for disinfection of dental prosthesis, less than 60% of the denture wearers use anyone due to the cost of the product 25 .…”