Pool of bovine morphogenetic proteins and guided tissue regeneration in the treatment of intrabony periodontal defects: I- Clinical measurements

Guimarães, Maria do Carmo Machado; Passanezi, Euloir; Sant’Ana, Adriana Campos Passanezi; Greghi, Sebastião Luiz Aguiar

doi:10.1590/s1678-77572004000100013

Cited by 14 publications

(12 citation statements)

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“…In this study, we utilized Wistar rats as the experimental model, considering the positive bone tissue response characteristics in this mammal, which resemble those found in humans, and also the ease of acquiring and handling these animals. 7,8,10,11 When the animals in this study were analyzed on the seventh day after creating the bone defects, it was observed that Group 1 (treated with laser associated with BMPs) was at a more advanced stage of repair than the other groups studied, presenting an area of vessel neoformation, neoformed bone tissue with a large concentration of osteoblasts and absence of inflammatory reaction. On the other hand, in the other groups the presence of inflammatory reaction and necrosed tissue (Group 4 only) was very evident.…”

Section: Discussionmentioning

confidence: 89%

Effect of 650 nm low-power laser on bone morphogenetic protein in bone defects induced in rat femors<A NAME="volta1"></A>

et al. 2006

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Effect of 650 nm low-power laser on bone morphogenetic protein in bone defects induced in rat femors Effect of 650 nm low-power laser on bone morphogenetic protein in bone defects induced in rat femors 1 Efeito do laser de baixa potência 650 nm sobre proteínas morfogenéticas ósseas em defeitos ósseos induzidos em fêmores ratos ABSTRACT Purpose:To investigate the influence of 650 nm GaAlAs laser on the action of bone morphogenetic protein (BMP) in bone defects produced in rat femurs. Methods: The sample consisted of 12 male albino Wistar rats (Rattus norvegicus). The animals were randomly divided into four experimental groups. After undergoing anesthesia, the fur was removed from the lateral face of the right thigh and surgical dissection was performed to view the femur region. A bone defect was created using a spherical diamond-tipped drill bit. In groups 1 and 2, the defect was filled with a paste of Gen-Tech bone-inducing substance. The animals were treated with GaAlAs laser, at a predetermined dose of joules/cm 2 for 80 seconds, over an area of 1 cm 2 . Groups 2 and 4 were used as controls. Bone samples were removed to perform histological procedures and morphometric analyses on the 7 th , 14 th and 21 st days after the operation. The results obtained were subjected to statistical analysis using ANOVA variance according to two criteria, with four repetitions, followed by the post hoc t test. The rejection level for the nullity hypothesis was 0.05 or 5% (α ≤ 0.05). Results: In comparisons between G1, G2, G3 and G4, p = 0.024 was observed. In statistical comparisons using the t test for paired samples, only G1 vs. G4 presented a statistically significant result (p = 0.021). Conclusion: The association of low-power laser application and Gen-Tech bone-inducing substance achieved a better result than laser application alone or BMP use alone. Key words: Laser Therapy, Low-Level . Bone remodeling. Bone morphogenetic protein. RESUMOObjetivo: Este trabalho tem como objetivo estudar comparativamente a influência do laser AsGaAl de 650nm sobre a ação das proteínas morfogenéticas ósseas( BMP) em defeitos ósseos produzidas em fêmures de ratos. Metodologia: Utilizamos uma amostra composta por 12 ratos machos (Rattus norvegicus), de linhagem Wistar albino, mantidos confinados em caixas com temperatura ambiente constante e iluminação adequada. Os animais foram divididos aleatoriamente em 4 grupos experimentais. Após o procedimento anestésico, foi realizada a retirada dos pelos da face lateral da coxa direita, seguida de procedimento cirúrgico para permitir a visualização da região. Foi realizado um defeito ósseo empregando brocas diamantadas do tipo esférica. Nos grupos 1 e 2 o defeito foi preenchido com uma pasta formada por substância osteoindutora Gen -Tech. Os animais foram tratados com o Laser GaAlAs, com dose pré-determinada de 4 joules/cm 2 e tempo de 80 segundos para uma área de 1cm 2 . Os grupos 2 e 4 foram adotados como grupo-controle. As amostras do osso foram retiradas para realização de procedimento histológico...

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Section: Discussionmentioning

confidence: 89%

Effect of 650 nm low-power laser on bone morphogenetic protein in bone defects induced in rat femors<A NAME="volta1"></A>

et al. 2006

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“…Therefore, other carriers that produced better results must be used for testing the pBMPs studied here. The use of a membrane barrier associated or not associated with osteoinductive materials is a current procedure in dentistry 25,26 . Also, it has been used in orthopedic surgery 13,27 , being a center of interest in this study.…”

Section: Discussionmentioning

confidence: 99%

“…The results showed that its presence was not seen histologically at 30 days after surgery in both treatments. Collagen membrane has shown satisfactory results in dentistry 25,26 and in treatment of nonsegmental defects experimentally produced in flat bones of several laboratory animals 18,28 . Despite that, in segmental defects, there is a need for better controlled studies to establish the membrane influence in bone formation.…”

Section: Discussionmentioning

confidence: 99%

Assessment of bovine biomaterials containing bone morphogenetic proteins bound to absorbable hydroxyapatite in rabbit segmental bone defects

et al. 2006

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PURPOSE: To evaluate the osteo-regenerative capacity of two proprietary bone grafting materials, using a segmental defect model in both radial diaphyses of rabbits. METHODS: The right defect was filled with pooled bone morphogenetic proteins (pBMPs) bound to absorbable ultrathin powdered hydroxyapatite (HA) mixed with inorganic and demineralized bone matrix and bone-derived collagen, derived from bovine bone (Group A). The left defect was filled with bovine demineralized bone matrix and pBMPs bound to absorbable ultrathin powdered HA (Group B). In both groups, an absorbable membrane of demineralized bovine cortical was used to retain the biomaterials in the bone defects, and to guide the tissue regeneration. The rabbits were euthanized 30, 90 and 150 days after surgery. Radiographic, tomographic and histologic evaluations were carried out on all specimens. RESULTS: At 30 days, the demineralized cortical bone cover was totally resorbed in both groups. HA was totally resorbed from Group A defects, whereas HA persisted in Group B defects. A prominent foreign body reaction was evident with both products, more pronounced in sections from Group B. At 90 days, the defects in Group B exhibited more new bone than Group A. However, at 150 days after surgery, neither treatment had stimulated complete repair of the defect. CONCLUSION: The partial bone healing of the segmental defect occurred with low or none performance of the biomaterials tested.

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“…Quando se associa a BMP com um carreador, propicia-se imobilidade da proteína na área, diminui-se a quantidade de BMP necessária e o tempo para que ocorra a indução 45 . Dentre os materiais testados em associação à BMP, ressaltamos: hidroxiapatita 35,46 ; esponjas de colágeno reabsorvível 16,21,25,35,[46][47][48] ; politetrafluoretileno expandido 49,50 ; osso bovino 16,21,[51][52][53][54] e osso autógeno 55 . Os carreadores mais utilizados são a hidroxiapatita e o colágeno, pois são compostos presentes na estrutura óssea, absorvendo naturalmente a BMP 56 .…”

Section: Revisão De Literaturaunclassified

Aplicabilidade de rhBMP-2 em diferentes técnicas de enxertia

Pasquali

Casagrande

Ponte

et al. 2015

Rev. da Fac. de Odontologia, UPF

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IntroduçãoTécnicas de enxerto ósseo surgiram para suprir perdas de volume nos sentidos horizontais e verticais, em pacientes com quantidade óssea reduzida, visto que o sucesso de reabilitações bucofaciais depende não somente do treinamento e da experiên-cia do profissional, mas também da quantidade e da densidade de osso disponível 1,2 . O tecido ósseo tem as seguintes propriedades de reparação e formação: osteogênese que é a formação óssea mediada por osteoblastos; osteoindução, mediada por BMPs, induzindo a formação óssea ectópica por meio de células mesenquimais; e osteocondução, relacionado à composição e à estrutura (arcabouço). Tais propriedades têm papel importante nos diferentes mecanismos de formação óssea dos enxertos, os quais podem ter diferentes origens: autógenos, homógeno, xenógeno e aloplásticos [3][4][5][6] . Osso autógeno ainda é o "padrão ouro" para procedimentos de reconstrução 7 , apesar da grande morbidade 8 . Em estudos recentes, pesquisadores isolaram a principal proteína para regeneração óssea, a proteína morfogenética (BMP), e derivaram sinteticamente a Proteínas Morfogenéticas Ósseas Recombinantes Humanas (rhBMP-2). Essa proteína funciona como regulador de crescimento e diferenciação celular, induzindo a transformação de células-tronco em células produtoras de tecidos ósseos ou vasculares 3,6 . Objetivo: analisar a influência da rhBMP-2 em enxertos bucofaciais, apresentando pesquisas recentes, feitas em animais e em humanos, bem como as diferentes metodologias de aplicabilidade clínica e os resultados obtidos por seus autores. Revisão de literatura: para que seja possível a instalação de implantes ou a cor-

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Pool of bovine morphogenetic proteins and guided tissue regeneration in the treatment of intrabony periodontal defects: I- Clinical measurements

Cited by 14 publications

References 24 publications

Effect of 650 nm low-power laser on bone morphogenetic protein in bone defects induced in rat femors<A NAME="volta1"></A>

Effect of 650 nm low-power laser on bone morphogenetic protein in bone defects induced in rat femors<A NAME="volta1"></A>

Assessment of bovine biomaterials containing bone morphogenetic proteins bound to absorbable hydroxyapatite in rabbit segmental bone defects

Aplicabilidade de rhBMP-2 em diferentes técnicas de enxertia

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