Plot size and number of replicates for experiments with forage sorghum

Toebe, Marcos; Filho, Alberto Cargnelutti; Souza, Renato Santos de; Soares, Franciéle dos Santos; Mello, Anderson Chuquel; Melo, Patrícia Jesus de; Silva, Lucas Santos da

doi:10.1590/s1678-3921.pab2022.v57.02979

Cited by 1 publication

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References 14 publications

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“…The main methods used include the maximum curvature method and the Hatheway method, which are both based on the relationship between the coefficient of variation and the area of the plot. The difference with this study is that the analysis of the coefficient of variation here considers soil moisture, not soil fertility or grain yield [19][20][21][22]. In relation to this, Su Jiakai has described in detail the determination of the area of experimental plots' forage grass in several papers published in Acta Agrestia Sinica; they concluded that the small experimental plots used in many articles led to underrepresentative results, but the methods of determining plot area are limited to information in college textbooks, industry standards, and various industry texts [23], and there is no standard scientific method.…”

Section: Discussionmentioning

confidence: 94%

Plot Layout Method of Field Experiment for Wheat with Border Irrigation Based on Soil Water Content Heterogeneity

Song,

Shen,

Wang

et al. 2023

Agronomy

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The objective of this research was to improve the accuracy and representativeness of experimental plot studies by determining the optimum plot area and replication number for winter wheat with border irrigation. Considering the spatial distribution of soil water content, the border effect in relation to crop growth, and the lateral seepage of soil water, we sought to study and optimize the area and specifications of irrigation experiment plots with different levels and replicates. The results show that the experimental irrigation plot consisted of two parts—the core area and the guard area. The most suitable area for the experiment plot core area, with a single level and without replicates, was 60–80 m2. The core experimental area can be arranged with two replicates per 40 m2, with differences in soil moisture content between the treatments reaching more than 15% at the two experiment levels. Each plot comprised two replicates, or if they were 20 m2, then they contained three replicates; when the soil moisture contents differed between 10% and 15%, the area of each replicate plot was 80 m2, comprising two replicates, or 30 m2 with three replicates. When the difference in soil moisture content between the treatments exceeded 15% with the three experimental levels, the area of each plot was 30 m2 and they contained two replicates, or 20 m2 containing three replicates; at differences of 10% to 15%, each replicate plot was 50 m2 containing two replicates, or 30 m2 with three replicates. The experimental plots were rectangular, with irrigation furrows dug lengthwise; therefore, the plots had aspect ratios between 7:1 and 5:1. The width of the buffer area was over 60 cm. The effect of the border on plant height and LAI for winter wheat primarily emerged with one to three rows (20–60 cm) at the jointing stage, while the effect on grain yield and biomass in winter wheat mainly emerged with one to two rows (20–40 cm). The conclusions of this research will inform the development of surface irrigation methods for silt loam in northern Henan, as a reference for optimizing experiment plots employing border irrigation with different soil textures.

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Section: Discussionmentioning

confidence: 94%