Characterization of the deposition of collagen fibers and Lithogenic potential in bladder of rats submitted to a sugar cane biopolymer graft

Abstract

ARTIcLE INFO _________________________________________________________ ___________________Objectives: Suture materials are widely used in urology. The interaction of these materials with the extracellular matrix in the inflammatory process can be estimated by stereology of collagen fibers and the present study was designed to determine the behavior of the bladder tissue of rats to grafts of the biopolymer of sugar cane (BPCA), and the inflammation and intravesical stone formation compared to the polyglactin 910. Materials and Methods: 42 Wistar rats were divided in four groups: Group I (n = 10) rats submitted to bladder implantation of ~4-0 BPCA suture graft and euthanized at 4 weeks; Group II (n = 10) rats submitted to bladder implantation of 4-0 polyglactin 910 suture graft and euthanized at 4 weeks; Group III (n = 12) rats submitted to bladder implantation of ~4-0 BPCA suture graft and euthanized at 8 weeks; Group IV (n = 10) rats submitted to bladder implantation of 4-0 polyglactin 910 suture graft and euthanized at 8 weeks. Bladders collected at necropsy were analyzed for their weight and the presence of grafts and calculi. Sections were prepared for stereological analysis of collagen fibers. Results: The bladder weight was higher in group I, particularly in the presence of bladder stones. The presence of the graft was observed in 100% (group I), 80% (group II), 91.6% (group III) and 30% (group IV); polyglactin 910 showed an absorption of 70% in this period. The stereological analysis showed a higher volume density of collagen fibers in group I versus other groups (p < 0.001). Conclusion:The BPCA was a material with good integration into the bladder of rats; its absorption was slower than that of the polyglactin 910. The presence of urinary stones was lower in bladders with implantation of BPCA, particularly after 8 weeks. There was a greater initial inflammatory response to BPCA graft that was directly related to the increase in bladder weight and the presence of urinary stones, but that equalized the results of polyglactin 910 after 8 weeks.

A New Biomaterial for Urinary Catheters

“…Probably, this was the reason of SCB biodegradation in one SCB-6 group animal. This fact coincides with Carvalho Júnior's study [17]. Also there was no trace of SCB thread in one animal.…”

Non-clinical safety study of a sugarcane bacterial cellulose hydrogel

“…BC hydrogel subcutaneous implants were found beneath the hypodermis layer of the skin, a slight inflammatory response was present and the formation of new vessels (neovascularization) was present in the majority of the animals. These effects are supported by previous studies (Rangel et al, 2006;Carvalho Junior et al, 2012;Cordeiro-Barbosa et al, 2012;Lima et al, 2015;Pita et al, 2015;Lima et al, 2017;Cavalcante et al, 2018;Gonçalves-Pimentel et al, 2018).…”

Sugarcane Biopolymer as a Guide Tube in the Regeneration of Peripheral Nerves in Rats

“…In experimental studies the application of sugarcane biopolymer has shown adequate response in reconstruction of tympanic membrane (Mayer et al, 2011), fold vocal (Vasconcelos et al, 2015), osteochondral defect (Albuquerque et al), patch vascular (Marques et al, 2007), eviscerated eyes (Cordeiro-Barbosa et al, 2012) and bladder graft (Carvalho Junior et al, 2012). However, there are not records in scientific literature on the use this biopolymer in the treatment of nerve injuries.…”