Biological roles of filamin a in prostate cancer cells

Abstract

Objective: This study aims to investigate the association of fi lamin A with the function and morphology of prostate cancer (PCa) cells, and explore the role of fi lamin A in the development of PCa, in order to analyze its signifi cance in the evolvement of PCa. Materials and Methods: A stably transfected cell line, in which fi lamin A expression was suppressed by RNA interference, was fi rst established. Then, the effects of the suppression of fi lamin A gene expression on the biological characteristics of human PCa LNCaP cells were observed through cell morphology, in vitro cell growth curve, soft agar cloning assay, and scratch test. Results: A cell line model with a low expression of fi lamin A was successfully constructed on the basis of LNCaP cells. The morphology of cells transfected with plasmid pSilencer-fi lamin A was the following: Cells were loosely arranged, had less connection with each other, had fewer tentacles, and presented a fi brous look. The growth rate of LNCap cells was faster than cells transfected with plasmid pSilencer-fi lamin A (P <0.05). The clones of LNCap cells in the soft agar cloning assay was signifi cantly fewer than that of cells stably transfected with plasmid pSilencer-fi lamin A (P <0.05). Cells stably transfected with plasmid pSilencer-fi lamin A presented with a stronger healing and migration ability compared to LNCap cells (healing rate was 32.2% and 12.1%, respectively; P <0.05). Conclusion: The expression of the fi lamin A gene inhibited the malignant development of LNCap cells. Therefore, the fi lamin A gene may be a tumor suppressor gene.

Periodontal disease and the risk of prostate cancer: a meta-analysis of cohort studies

“…As the etiology of prostate cancer develops, increasing evidence suggests that chronic or recurrent inflammation may also be associated with prostate cancer risk. Moreover, the low level of persistent systemic inflammation caused by periodontal disease can induce oxidative DNA damage, uncontrolled repair procedures and eventually the occurrence of tumors in the body (31)(32)(33). Therefore, if a genetic link can be determined between periodontal disease and prostate cancer, the specific identification of genetic polymorphism may be beneficial to identifying high-risk groups and developing preventive strategies, which merits further attention.…”