Cystometric analysis of the transplanted bladder

Abstract

Objective Cystometric evaluation of the bladder after autotransplant and isogeneic transplant in female rats.Material and Methods Two groups were constituted: (A) bladder autotransplant with two subgroups: R1 – (control) and R2 – (bladder transplant); (B) isogeneic bladder transplant with three subgroups; T1 – (control); T2–T3, two subgroups observed for 30 and 60 days after transplant, respectively. All animals underwent cystometric evaluation. Afterwards, the bladders were removed for histological study.Results The transplanted bladders did not show significant changes in filling/storage and emptying/micturition functions after 30 and 60 days of evolution. Upon macroscopical evaluation, there was good revascularization and the tissue was well preserved. Cystometry results: Did not show significant differences in the micturition pressure in subgroups T2-T3, but did between subgroups R1−R2, T1−T2, and T1−T3. Significant differences were verified in the micturition interval between T1−T3, T2−T3, but not between R1−R2, T1−T2. There was significant difference in the micturition duration between T1−T3 but not between R1−R2, T1−T2 and T2−T3. No fistula was noted on the suture site nor leakage of urine in the abdominal cavity or signs of necrosis or retraction were observed.Conclusions Transplant of the bladder was shown to be a viable procedure. The results indicate that there was structural and functional regeneration of transplanted bladders, and these results indicate that it is possible that vascular endothelium growth and neurogenesis factors are involved and activated in the process of the preservation or survival of the transplanted organ.

Introducing a novel experimental model of bladder transplantation in mice

“…However, these tests employed in metabolic cages cannot determine whether the Trp mice were able to completely empty their bladder (bladder voiding efficiency). To further evaluate test the bladder function and voiding efficiency, we subjected Ctrl and Trp mice to cystometry 13,14 . Briefly, a catheter was inserted into the bladder under microscopic guidance (Figure 2D).…”

“…To further evaluate test the bladder function and voiding efficiency, we subjected Ctrl and Trp mice to cystometry. 13,14 Briefly, a catheter was inserted into the bladder under microscopic guidance ( Figure 2D). Two weeks after catheter insertion, the mice were placed on the cystometry machine, saline was steadily infused into the bladder through inserted catheter, and voiding pressure and volume were automatically monitored for more than 2 hours.…”

Editorial Comment

“…The form, vascularity and survival remain hypothetical but there is no promise of function as the graft has no neural connection proposed. In a rat model supra trigonal cystectomy and isogenic transplant did show functional recovery; 4 however, the rat has the ability to regenerate bladder and function. 5 Extrapolation in humans is to be determined.…”

New frontiers in urologic organ transplantation

“…There has been one reported case of transplanting a human urinary bladder en bloc with pediatric kidney transplantation, but that was back in 2008 3 . A 2017 Brazilian study reported feasibility of bladder transplants in rats 4 . While the path from proof of concept in a mouse model to clinical use in human patients is obviously long and uncertain, the mouse model presented in this edition of the AJT provides a good starting place.…”