“…The activities of the cytosolic -galactosidases ( -gal I and II) and cell wall -galactosidase ( -gal III) as a function of the pH of the reaction medium, showed an increase from pH 2.5 until reaching maximum activity at pH 4.0, with a progressive decrease to pH 6.5 (Figure 1). The optimal pH activity range demonstrated by -gal I, II and III is common to a wide variety of plant species, especially legumes (ALCÂNTARA et al, 2006;BISWAS;KAYASTHA;SECKLER, 2003;CHILAKA;OKEKE;ADAIKPOH, 2002;ENÉAS-FILHO et al, 2000;ENÉAS-FILHO et al, 2001;SUDÉRIO et al, 2011b). The optimum test temperature for the -gal I, II and III enzymes was 55 °C, with a considerable drop in activity from 60 °C.…”