Coffee biotechnology

Santos-Briones, César De los; Hernández‐Sotomayor, S.M. Teresa

doi:10.1590/s1677-04202006000100015

Cited by 22 publications

(12 citation statements)

References 54 publications

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“…The low concentration of endophytes in the seeds [25,30] and the development of new embryos by adventive embryogenesis increases the chance of developing Burkholderia -free plants. In Coffea arabica (Rubiaceae), it occurs uncommonly in vitro , but it is mostly induced during plant cultivation with plant hormones (2,4-dichlorophenoxyacetic acid or naphthalene acetic acid) [51,52]. We observed adventive embryogenesis in 10–30% of the developing embryos after EC in the absence of plant hormones.…”

Section: Discussionmentioning

confidence: 87%

Is the bacterial leaf nodule symbiosis obligate for Psychotria umbellata? The development of a Burkholderia-free host plant

et al. 2019

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Background & aims The bacterial leaf nodule symbiosis is an interaction where bacteria are housed in specialised structures in the leaves of their plant host. In the Rubiaceae plant family, host plants interact with Burkholderia bacteria. This interaction might play a role in the host plant defence system. It is unique due to its high specificity; the vertical transmission of the endophyte to the next generation of the host plant; and its supposedly obligatory character. Although previous attempts have been made to investigate this obligatory character by developing Burkholderia -free plants, none have succeeded and nodulating plants were still produced. In order to investigate the obligatory character of this endosymbiosis, our aims were to develop Burkholderia- free Psychotria umbellata plants and to investigate the effect of the absence of the endophytes on the host in a controlled environment. Methods The Burkholderia- free plants were obtained via embryo culture, a plant cultivation technique. In order to analyse the endophyte-free status, we screened the plants morphologically, microscopically and molecularly over a period of three years. To characterise the phenotype and growth of the in vitro aposymbiotic plants, we compared the growth of the Burkholderia -free plants to the nodulating plants under the same in vitro conditions. Key results All the developed plants were Burkholderia -free and survived in a sterile in vitro environment. The growth analysis showed that plants without endophytes had a slower development. Conclusions Embryo culture is a cultivation technique with a high success rate for the development of Burkholderia -free plants of P . umbellata . The increased growth rate in vitro when the specific endophyte is present cannot be explained by possible benefits put forward in previous studies. This might indicate that the benefits of the endosymbiosis are not yet completely understood.

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Section: Discussionmentioning

confidence: 87%

Is the bacterial leaf nodule symbiosis obligate for Psychotria umbellata? The development of a Burkholderia-free host plant

et al. 2019

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“…Coffee quality, which determines its marketability and price, is a complex trait resulting from multifactorial determinants such as plant genetics, pedoclimatic conditions, and pre-and postharvest treatments like roasting and brewing (Martin et al 1999;Montavon et al 2003;De Los Santos-Briones and Hernández-Sotomayor 2006;Leroy et al 2006). Although most of the studies directed at improving coffee quality have focused on physiological and agronomic aspects of the coffee plant (Montavon et al 2003;Leroy et al 2006), the microbiota associated with coffee plants may play a critical role in the final expression of coffee quality (Pasin et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Endophytic microbial diversity in coffee cherries ofCoffea arabicafrom southeastern Brazil

et al. 2013

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The microbiota associated with coffee plants may play a critical role in the final expression of coffee quality. However, the microbial diversity in coffee cherries is still poorly characterized. Here, we investigated the endophytic diversity in cherries of Coffea arabica by using culture-independent approaches to identify the associated microbes, ultimately to better understand their ecology and potential role in determining coffee quality. Group-specific 16S rRNA and 26S rRNA genes polymerase chain reaction - denaturing gradient gel electrophoresis and clone library sequencing showed that the endophytic community is composed of members of the 3 domains of life. Bacterial sequences showing high similarity with cultured and uncultured bacteria belonged to the Betaproteobacteria, Gammaproteobacteria, and Firmicutes phyla. Phylogenetic analyses of cloned sequences from Firmicutes revealed that most sequences fell into 3 major genera: Bacillus, Staphylococcus, and Paenibacillus. Archaeal sequences revealed the presence of operational taxonomic units belonging to Euryarchaeota and Crenarchaeota phyla. Sequences from endophytic yeast were not recovered, but various distinct sequences showing high identity with filamentous fungi were found. There was no obvious correlation between the microbial composition and cultivar or geographic location of the coffee plant. To the best of our knowledge, this is the first report demonstrating internal tissue colonization of plant fruits by members of the Archaea domain. The finding of archaeal small-subunit rRNA in coffee cherries, although not sufficient to indicate their role as active endophytes, certainly expands our perspectives toward considering members of this domain as potential endophytic microbes.

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“…Coffee is an agricultural crop of significant economic importance. Coffea arabica L. (arabica type coffee) is typical of the highland growing regions and is responsible for almost 75% of world production [ 44 ]. In this study, we report the validation of housekeeping genes to identify the most suitable internal reference gene(s) for normalization of qPCR data obtained among five different tissues/organs (root, stem, leaf, flower, and fruits) of C. arabica .…”

Section: Introductionmentioning

confidence: 99%

Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions

Barsalobres-Cavallari

et al. 2009

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Background: Quantitative data from gene expression experiments are often normalized by transcription levels of reference or housekeeping genes. An inherent assumption for their use is that the expression of these genes is highly uniform in living organisms during various phases of development, in different cell types and under diverse environmental conditions. To date, the validation of reference genes in plants has received very little attention and suitable reference genes have not been defined for a great number of crop species including Coffea arabica. The aim of the research reported herein was to compare the relative expression of a set of potential reference genes across different types of tissue/organ samples of coffee. We also validated the expression profiles of the selected reference genes at various stages of development and under a specific biotic stress.

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Coffee biotechnology

Cited by 22 publications

References 54 publications

Is the bacterial leaf nodule symbiosis obligate for Psychotria umbellata? The development of a Burkholderia-free host plant

Is the bacterial leaf nodule symbiosis obligate for Psychotria umbellata? The development of a Burkholderia-free host plant

Endophytic microbial diversity in coffee cherries ofCoffea arabicafrom southeastern Brazil

Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions

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