2006
DOI: 10.1590/s1677-04202006000100008
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Abstract: Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to the Rubiaceae family which includes other important plants. The genus contains about 100 species but commercial production is based only on two species, Coffea arabica and Coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. The Brazilian Coffee Genome Project was designed with the objective of making modern genomics resources availa… Show more

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Cited by 114 publications
(119 citation statements)
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References 44 publications
(34 reference statements)
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“…Six of these microsatellites, or simple sequence repeats (SSR), were identified in DNA clones derived from genomic libraries and obtained from Combes et al (2000) and Rovelli et al (2000). Two expressed sequence tags (EST) were developed from cluster consensus sequences derived from the Coffee Genome Project (Vieira et al, 2006) using the TROLL software (Castelo et al, 2002). Every forward primer was labeled with either blue (FAM) or green (JOE) fluorescent tags (Invitrogen, São Paulo, Brazil).…”
Section: Microsatellite Marker Assaymentioning
confidence: 99%
“…Six of these microsatellites, or simple sequence repeats (SSR), were identified in DNA clones derived from genomic libraries and obtained from Combes et al (2000) and Rovelli et al (2000). Two expressed sequence tags (EST) were developed from cluster consensus sequences derived from the Coffee Genome Project (Vieira et al, 2006) using the TROLL software (Castelo et al, 2002). Every forward primer was labeled with either blue (FAM) or green (JOE) fluorescent tags (Invitrogen, São Paulo, Brazil).…”
Section: Microsatellite Marker Assaymentioning
confidence: 99%
“…The search for polymorphic SSR loci associated with a desirable trait is complex due to the limited number of coffee SSRs available. In species with a narrow genetic base, such as C. arabica, the probability of finding polymorphism is low (Baruah et al, 2003;Vieira et al, 2006). Genetic diversity among coffee species identified by EST-SSR markers can be used as a gene source in breeding programs, with potential for other studies of genetic mapping, in the identification of cultivars or in pre-breeding by the genotyping of germplasm bank accessions.…”
Section: Resultsmentioning
confidence: 99%
“…EST-SSR have some intrinsic advantages in that they are quick to elucidate -by electronic sorting -, abundant, unbiased in repeat type and present in gene-rich areas (Scott, 2001). The Brazilian Coffee EST database has more than 200 thousand DNA sequences (Vieira et al, 2006).…”
Section: Introductionmentioning
confidence: 99%
“…The raw digital gene expression counts were normalized using the reads per kilobase of transcript per million mapped reads (Mortazavi et al, 2008) method to correct the digital gene expression counts for bias caused by sequence size and total EST numbers in each cDNA library described in a previous report (Vieira et al, 2006). These values were used to generate a heatmap with the Genesis software, version 1.7.6 (Sturn et al, 2002).…”
Section: In Silico Expression Pattern Analysismentioning
confidence: 99%
“…We observed high gene expression in leaves and low levels in flowers and in fruits collected at different DAF, corroborating the in silico approach (Figure 4). (Vieira et al, 2006;Mondego et al, 2011): seedlings and leaves treated with arachidonic acid (AR1); suspension cells treated with acibenzolar-S-methyl (BP1); non-embryogenic calli with and without 2,4 dichlorophenoxyacetic acid (CA1, IC, and PC); suspension cells treated with acibenzolar-S-methyl and brassinosteroids (CB1); hypocotyls treated with acibenzolar-S-methyl (CL2); suspension cells treated with NaCl (CS1); embryogenic calli (EA1 and IAc); flower buds in different developmental stages (FB); flower buds + pinhead fruits + fruits at different stages (FR); seedlings and leaves treated with arachidonic acid (LP1); young leaves from the orthotropic branch (LV1); mature leaves from plagiotropic branches (LV2); primary embryogenic calli (PA1); leaves infected with leaf miner and coffee leaf rust (RM1); roots with acibenzolar-S-methyl (RT5); suspension cells stressed with aluminum (RT8); stems infected with Xylella spp (RX1); water deficit-stressed field plants (pool of tissues) (SH2); and germinating seeds (whole seeds and zygotic embryos) (SI3).…”
Section: Cacyp81d8_1 Transcriptional Profile Validationmentioning
confidence: 99%