Prevalência de Chlamydia trachomathis em amostras endocervicais de mulheres em São Paulo e Santa Catarina pela PCR

Herkenhoff, Marcos Edgar; Gaulke, Rodrigo; Vieira, Luisa Lemos; Ferreira, Polyanna da Silva; Pitlovanciv, Ana Kelly; Remualdo, Vanessa Rosália

doi:10.1590/s1676-24442012000500004

Cited by 5 publications

(4 citation statements)

References 25 publications

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“…In this study, there was a high number of positive cases (24.39%) of CT in young people aged 15-25 years. This prevalence was higher than that found by Araújo in the city of Goiania, in adolescents and young women, estimated at 19.6% and lower than those found in São Paulo and Santa Catarina (27,28) . Statistical analyses of this trial demonstrated an association of the variable age with the risk of Chlamydia infection (p=0.0060).…”

Section: Discussioncontrasting

confidence: 67%

“…The overall rates for each of the cities participating in the study, in descending order, were: Rio de Janeiro (15%), Porto Alegre (12.2%), Vitória (10.7%), São Paulo (9.1%), Manaus (7.8%), Goiânia (7.6% in the female population and 5% in asymptomatic males), and Fortaleza (4.7% for both sexes) (15,(22)(23)(24)(25)(26) . More recent data have established a prevalence of 56.45% in the endocervical samples from 287 women in São Paulo and Santa Catarina by the PCR method (27) . In another study, conducted in Manaus, with samples from 100 pregnant women by the same method demonstrated positivity of 11% (28) .…”

Section: Discussionmentioning

confidence: 99%

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Detection of chlamydia trachomatis by immunological methods in adult and adolescent female population in Cuiabá, Mato Grosso

Matos¹,

Machado²,

Zavala³

et al. 2015

J Bras Doenças Sex Transm

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ARTICLE INTRODUCTIONThe WHO estimates that there are 105.7 million new cases of Chlamydia worldwide, with 3 to 4 million of these only in the United States of America (USA), with over 1.4 million cases reported in 2011 (1)(2)(3)(4)(5) , 5 million in Eastern Europe, and 34 million in subSaharan Africa and southeast Asia (5)(6)(7)(8)(9) . Worldwide, it occurs more frequently in sexually active young adults, generally aged younger than 20 years, and it is nearly three times higher in the 14-24 years age group (5,7,9,10) . It currently represents the leading cause of female infertility, but the most common infections are urethritis and cervicitis, which, if untreated, can lead to serious consequences in the reproductive tract such as ectopic pregnancy and infertility (6,(8)(9)(10) . About 50% of infected men and 70 to 75% of infected women are asymptomatic. The infection is transmitted during sexual contact and to the newborn at birth and may cause neonatal conjunctivitis or pneumonia (5,6,(8)(9)(10) . Over 100 million individuals of both sexes worldwide are infected with Chlamydia at some point in their lives (6,9,10) . Among male subjects, the prevalence is comparable with that of risk populations, ranging from 15 to 20%, while in the asymptomatic population, it is between 3 and 7%. In female subjects, the infection rates of asymptomatic populations are between 3 and 10%, while in high-risk populations they are above 20% (1)

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Section: Discussioncontrasting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Detection of chlamydia trachomatis by immunological methods in adult and adolescent female population in Cuiabá, Mato Grosso

Matos¹,

Machado²,

Zavala³

et al. 2015

J Bras Doenças Sex Transm

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“…[12][13][14][15][16][17] The laboratory diagnosis of C. trachomatis can be performed by traditional methods ranging from isolation in cell culture to nucleic acid detection; however, technical difficulties remain, but once overcome, the sensitivity and specificity standards of diagnostic methods may be improved. [18][19][20] In Brazil, the gold standard for characterizing the infection is isolation of C. trachomatis in cell cultures, a methodology that is rarely used (sensitivity of 70-90%), as it requires specialized personnel, a specific laboratory and strict requirements for collection, transportation and handling of the samples. [21][22][23][24] Direct immunofluorescence is another technique used, but readings of the results under the immunofluorescence microscope are often difficult and subjective.…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting by Phage Display of Mimetic Peptides of Chlamydia trachomatis for Use in Laboratory Diagnosis

Freitas¹,

Queiroz²,

Machado³

et al. 2022

IDR

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Background Chlamydia trachomatis infection is a major public health problem and the most common sexually transmitted infection in the world. Although highly prevalent, 70% to 80% of cases are asymptomatic and undiagnosed. Purpose To overcome some limitations in terms of rapid diagnosis, phage display technology was used to bioprospect peptide mimetics of C. trachomatis immunoreactive and immunogenic antigens to be selected for the production of synthetic peptides. Methods Initially, IgG from 22 individuals with C. trachomatis and 30 negative controls was coupled to G protein magnetic beads. The phage display technique consisted of biopanning, genetic sequencing, bioinformatics analysis and phage ELISA. Results Clones G1, H5, C6 and H7 were selected for testing with individual samples positive and negative for C. trachomatis . Reactions were statistically significant (p < 0.05), with a sensitivity of 90.91, a specificity of 54.55, and AUC values >0.8. One-dimensional analysis with C. trachomatis components indicated that the G1 clone aligned with cell wall-associated hydrolase domain-containing protein, the H5 clone aligned with glycerol-3-phosphate acyltransferase PlsX protein, the C6 clone aligned with a transposase and inactivated derivatives, and the H7 clone aligned with GTP-binding protein. Molecular modeling and three-dimensional analysis indicated the best fit of the four clones with a protein known as chlamydial protease/proteasome-like activity factor (CPAF), an important virulence factor of the bacterium. Conclusion The peptides produced by phage display are related to the metabolic pathways of C. trachomatis , indicating that they can be used to understand the pathogenesis of the infection. Because of their high sensitivity and AUC values, the peptides present considerable potential for use in platforms for screening C. trachomatis infections.

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“…Este trabalho trata de uma revisão integrativa, onde foram pesquisados artigos que abordavam o tema proposto, usando-se os seguintes critérios de exclusões: ano de publicação (2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011)(2012)(2013)(2014)(2015)(2016)(2017)(2018) e os artigos que não apresentavam a relevância do assunto.…”

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Influência de Patologias na Fertilidade Feminina / Influence of Pathologies on Female Fertility

et al. 2019

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A infertilidade é a incapacidade do casal de ter um filho após um ano de relações sexuais sem métodos contraceptivos (preservativos, anticoncepcionais, dispositivo intrauterino (DIU), entre outras). Essa incapacidade ocorre devido a vários fatores como: patologias que acometem diretamente ou indiretamente o aparelho reprodutor, o estresse, a idade, doenças metabólicas e bem-estar psicológico. Este estudo revisa 27 artigos sobre patologias e sua influência na fertilidade feminina entre 2004 e 2018. Campanhas relacionadas a temática são necessárias para um maior esclarecimento da população.

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Prevalência de Chlamydia trachomathis em amostras endocervicais de mulheres em São Paulo e Santa Catarina pela PCR

Abstract: Primeira submissão em 27/11/11 Última submissão em 09/04/12 Aceito para publicação em 16/07/12 Publicado em 20/10/12

Cited by 5 publications

References 25 publications

Detection of chlamydia trachomatis by immunological methods in adult and adolescent female population in Cuiabá, Mato Grosso

Detection of chlamydia trachomatis by immunological methods in adult and adolescent female population in Cuiabá, Mato Grosso

Bioprospecting by Phage Display of Mimetic Peptides of Chlamydia trachomatis for Use in Laboratory Diagnosis

Influência de Patologias na Fertilidade Feminina / Influence of Pathologies on Female Fertility

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