HPV detection in oral carcinomas

Abstract

The authors set out in this study to verify the presence of low-and high-risk DNA of human papillomavirus (HPV) in oral cancer by means of the hybrid capture Digene ® test (São Paulo-SP, Brazil) in smears from exfoliative cytology and also to compare the findings with those of conventional light microscopy (hematoxylin-eosin (HE)/Papanicolaou). Forty individuals gave their written informed consent to participate in the study and also had their clinical data analyzed. The 40 exfoliative cytology examinations performed to date produced the following results: 29 (72.5%) negative for low-and high-risk HPV-DNA; nine (22.5%) positive for low-and high-risk HPV-DNA; one (2.5%) positive for low-risk HPV-DNA; and one (2.5%) positive for high-risk HPV-DNA. There was agreement among the findings for the presence of DNA-HPV for both exfoliative cytology (smear to hybrid capture Digene ® test and the cytological smear readings made by conventional light microscopy). It was therefore concluded that the HPV virus may be a cocarcinogen of the mouth cancer as it is in the cervix cancer. Oral cancer HPV Exfoliative cytology Hybrid capture resumo Os autores buscaram verificar, neste estudo, a presença do papilomavírus humano (HPV) de baixo e de alto risco em carcinomas orais através do teste de captura híbrida Digene ® (São Paulo-SP, Brasil) em amostras colhidas pela citologia esfoliativa bucal e, ainda, avaliar comparativamente as referidas leituras com alterações celulares indicativas deste vírus obtidas com a interpretação citológica óptica convencional (hematoxilinaeosina (HE)/Papanicolaou). Quarenta indivíduos concordaram, espontaneamente, através de assinatura do termo de consentimento livre e esclarecido, em participar da pesquisa, e seus dados clínicos foram analisados. Entre as 40 amostras provenientes da citologia esfoliativa 29 (72,5%) mostraram-se negativas para presença de HPV-DNA de baixo e de alto risco; nove (22,5%) foram positivas para o HPV-DNA de baixo e de alto risco; uma (2,5%) foi positiva apenas para o HPV de baixo risco; e também uma (2,5%) foi positiva apenas para o HPV de alto risco. Houve concordância entre todos os resultados positivos para presença de HPV-DNA nas amostras citológicas submetidas ao teste de captura híbrida Digene ® e na leitura de esfregaço citológico ao microscópio óptico convencional. Desta forma, conclui-se que o vírus HPV pode comportar-se como mais um co-carcinógeno para o câncer de boca, à semelhança do carcinoma uterino.

Human papillomavirus infection and premalignant lesions of the oral cavity: A cross‐sectional study in Allahabad, North India

“…On the basis of koilocytosis Vidal et al. reported 32.5% HPV positive cases and 67.5% negative ones 29 . Similarly, in our study evidence of koilocytosis was reported in 45.4% of the total cases on histopathology.…”

Correlation of addictive factors, human papilloma virus infection and histopathology of oral submucous fibrosis

“…from Brazil recently studied the role of HPV by this assay in patients with oral carcinoma and reported 22.5% positivity of high risk‐HPV. They also suggested that the detection of HPV by this technique not only helped to identify viral infections, but also corresponded to findings of koilocytosis on exfoliative cytology and concluded that presence of HPV might also contribute to the development of cancer, but there may be many other factors such as smoking, tobacco chewing and radiation that were consistently present in persons suffering from oral malignancies (16).…”

Role of human papillomavirus and its detection in potentially malignant and malignant head and neck lesions: updated review

“…Chen et al reported the positivity of HPV16 as 52.6% and HPV 18 as 25% in OSMF by the PCR method [ 67 ]. Vidal et al reported 72.5% negative for low and high risk HPV DNA, 22.5% positive for low and high risk HPV DNA, 2.5% positive for low risk HPV DNA and 2.5% positive for high risk HPV DNA in 40 oral carcinoma cases by the Hybrid Capture technique [ 68 ].…”