Detection of agents associated with respiratory diseases of swine by real time PCR

Fonseca, Antônio Augusto; Nonaka, Carolina Kymie Vasquez; Guedes, Estefânia de Oliveira; Lobato, Zélia Inês Portela; Dias, Anderson; Nascimento, J.A.F.B.; Klein, Cátia Silene; Reis, Jenner Karlisson Pimenta dos; Heinemann, Marcos Bryan

doi:10.1590/s1519-99402015000200005

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…Pneumonias represented the group of greatest importance in this work. The term "swine respiratory disease complex" is used to refer to the interaction of many infectious agents with risk factors for the development of pneumonia in pigs (Fonseca et al 2015). In the present study, this interaction was evident and was considered for the establishment of diagnoses.…”

Section: Discussionmentioning

confidence: 96%

Causes of death in growing-finishing pigs in two technified farms in southern Brazil

et al. 2020

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The aim of this study was to investigate the main causes of death in growing-finishing pigs in southern Brazil. During a one-year period (from 2018 to 2019), two industrial pig herds (18 and 20 thousand pigs each farm) in southern Brazil were monitored along the four seasons of the year (12 days per season on each farm), in order to perform necropsies of all pigs that died in that period. The two farms had an average monthly mortality rate ranging from 0.94 to 3.93% in the evaluated months. At necropsy, tissues were collected, fixed in 10% formalin solution and processed routinely for histopathological examination. When necessary, samples were sent for bacterial culture and PCR to identify etiologic agents. A total of 601 necropsies were performed, with 94.9% of conclusive diagnoses. Infectious diseases corresponded to 64.4% of conclusive diagnosis and non-infectious diseases to 35.6%. The most prevalent causes of death were: pneumonia (33%), gastric ulcers (15.4%), circovirosis (9.9%), systemic bacterial embolism (5.4%), polyserositis (4.4%), dilated cardiomyopathy and torsion of abdominal organs (4.3% each), and bacterial pericarditis (3.4%). Regarding pneumonias (199/601), the main agents identified in these cases were Pasteurella multocida, Influenza A virus and Mycoplasma hyopneumoniae, mainly in associations.

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Section: Discussionmentioning

confidence: 96%

Causes of death in growing-finishing pigs in two technified farms in southern Brazil

et al. 2020

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“…The animals were not treated with antibiotics. The negative cases might have been caused by other infectious agents such as Haemophilus parasuis , pseudorabies or Escherichia coli [32].…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of Streptococcus suis from sick pigs in Bali, Indonesia

et al. 2019

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ObjectiveStreptococcus suis (S. suis) is a causative agent for various syndromes in pigs. It can be transmitted to humans with typical symptoms of meningitis and death. Although human infections have been confirmed at Bali Referral Hospital, Indonesia, since 2014, the bacteria have not been isolated from pigs. Here, we provide confirmation of the presence of the bacteria in sick pigs in the province.ResultsStreptococcus suis was confirmed in 8 of 30 cases. The final confirmation was made using PCR and sequencing of the glutamate dehydrogenase (GDH) and recombination/repair protein (recN) gene fragments. Upon PCR serotyping, two were confirmed to be serotype 2 or 1/2. Prominent histopathological lesions of confirmed cases were meningitis, endocarditis, pericarditis, bronchopneumonia, enteritis and glomerulonephritis. The dominant inflammatory cells were neutrophils and macrophages. Further research is needed to understand the risk factors for human infection. Community awareness on the risk of contracting S. suis and vaccine development are needed to prevent human infections.

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“…It mainly affects finishing swine with morbidity rates ranging from 10% to 40% and mortality rates from 2% to 10%. The main agents involved in PRDC are Mycoplasma hyopneumoniae, porcine circovirus type 2 (PCV2) and swine influenza virus (Fonseca Júnior et al., 2015;Risco et al, 2015). These diseases are enzootic and are widespread in most Brazilian farms, causing high economic losses (Ciacci-Zanella, Morés, & Barcellos, 2016).…”

Section: Introductionmentioning

confidence: 99%

Health profile of free‐range wild boar ( Sus scrofa ) subpopulations hunted in Santa Catarina State, Brazil

Severo

Werlang

Mori

et al. 2020

Transbounding Emerging Dis

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Wild boars represent a potential dissemination source of important pathogens to public and animal health, since they can host several pathogens. This study aimed to evaluate the health profile of wild boars hunted for population control in the state of Santa Catarina (SC), south of Brazil. For this study, tissue and blood samples were collected from 61 wild boars hunted from October 2017 to November 2018. All 61 serum samples were screened for antibodies by different assays, presenting the following seroprevalence: 52.4% for porcine circovirus type 2 (PCV2); 21.3% for Leptospira spp.; 19.7% for Mycoplasma hyopneumoniae; 13.1% for hepatitis E virus (HEV); and 9.8% for influenza A virus (IAV). No seroreaction was observed for Brucella spp. and classical swine fever virus (CSFV). At necropsy, the main lesions observed were evidence of pulmonary metastrongylid parasites, haemorrhagic lymph nodes and white-spotted liver. Histopathological analyses mainly showed interstitial pneumonia, bronchopneumonia and bronchi-associated lymphoid tissue (BALT) hyperplasia. There was a significant association between metastrongylid parasitism and BALT hyperplasia in lungs. Pathological findings of wild boars indicate that the assessed individuals were not exposed to notifiable diseases, such as CSFV, African swine fever virus and foot-and-mouth disease, which cause economic damage by non-tariff trade barriers. However, the detection of antibodies against PCV2 and M. hyopneumoniae, pathogens that impact commercial production, indicates the circulation of these agents, with frequency variation in wild populations. This shows the potential risk of pathogen transmission among domestic swine populations and free-living ones. The detection of antibodies against zoonotic agents such as HEV, IAV and Leptospira spp., other than characterizing the pathogens' circulation in these populations, suggests a potential risk to public health and pig farming, depending on the interactions established with the environment, humans and domestic swine. K E Y W O R D S emerging diseases, necropsy, pig health, public health, wild boars How to cite this article: Severo DRT, Werlang RA, Mori AP, et al. Health profile of free-range wild boar (Sus scrofa) subpopulations hunted in Santa Catarina State, Brazil.

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Detection of agents associated with respiratory diseases of swine by real time PCR

Cited by 3 publications

References 15 publications

Causes of death in growing-finishing pigs in two technified farms in southern Brazil

Causes of death in growing-finishing pigs in two technified farms in southern Brazil

Isolation and identification of Streptococcus suis from sick pigs in Bali, Indonesia

Health profile of free‐range wild boar ( Sus scrofa ) subpopulations hunted in Santa Catarina State, Brazil

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