The emergence of multidrug-resistant strains to antibiotics has motivated the search for new substances with antimicrobial activity, especially those derived from medicinal plants. Salvia officinalis L. is a medicinal plant that arouses scientific interest due to being associated with multiple therapeutic effects. The purpose of this study was to evaluate the in vitro antimicrobial potential of S. officinalis L. against pathogens in the oral cavity. The antimicrobial potential of the ethanol extract of leaf of S. officinalis L was evaluated by broth microdilution, with determination of minimum inhibitory concentration (MIC) and Minimum bactericidal/Fungicide concentration (MBC / MFC), against the species Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius, Streptococcus sanguis, Candida albicans, Candida glabrata, Candida guillermond, Candida krusei and Candida tropicalis. The extract showed moderate antifungal potential before Candida species (MIC = 1 mg/mL). And for the species of Streptococcus, the antimicrobial activity was from moderate to strong whose MIC ranged from 0.25 to 1 mg/mL. In this study, the extract from the leaves of S. officinalis L. presented oral cavity antimicrobial activity against pathogens. These results point to S. officinalis as a possible source of active ingredients in the development of formulations with antimicrobial activity of dental use.