Effects of ethanol on the osteogenesis around porous hydroxyapatite implants

Lima, CC; Silva, T.-D.; Santos, Luiz Carlos Oliveira dos; Nakagaki, Wilson Romero; Loyola, Y.C.S.; Resck, M. C. C.; Camilli, José Ângelo; Soares, Evelise Aline; Garcia, José Antônio Dias

doi:10.1590/s1519-69842011000100017

Cited by 16 publications

(14 citation statements)

References 18 publications

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“…Studies on the effects of ethanol on bone tissue have used concentrations ranging from 5% to 20% for periods of 4 to 12 weeks 27 , 8 , 20 , 21 No reports associating the effects of ethanol and OTM were found; thus, we used 20% ethanol of which effects on bone neoformation are widely known 26 , 7 , 8 , 22 …”

Section: Discussionmentioning

confidence: 99%

The effects of binge-pattern alcohol consumption on orthodontic tooth movement

Araújo

Johann

Camargo

et al. 2014

Dental Press J. Orthod.

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OBJECTIVE: This study aimed to assess tissue changes during orthodontic movement after binge-pattern ethanol 20% exposure. METHODS: Male Wistar rats (n = 54) were divided into two groups. The control group (CG) received 0.9% saline solution, while the experimental group (EG) received 20% ethanol in 0.9% saline solution (3 g/kg/day). On the 30th day, a force of 25 cN was applied with a nickel-titanium closed coil spring to move the maxillary right first molar mesially. The groups were further divided into three subgroups (2, 14 and 28 days). Tartrate-resistant acid phosphatase and picrosirius were used to assess bone resorption and neoformation, respectively. Data were compared by two-way ANOVA, Tukey's HSD, Games-Howell and chi-square test. Significance level was set at 5%. RESULTS: There was a decrease in the number of osteoclasts in EG at day 28. The percentage of collagen showed no interaction between group and time. CONCLUSION: Binge-pattern 20% ethanol promoted less bone resorption at the end of tooth movement, thereby suggesting delay in tooth movement.

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Section: Discussionmentioning

confidence: 99%

The effects of binge-pattern alcohol consumption on orthodontic tooth movement

Araújo

Johann

Camargo

et al. 2014

Dental Press J. Orthod.

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“…Bone repair depends on migration, proliferation and differentiation of osteogenic cells into osteoblastic ones (Marks and Popoff, 1988) and this process can be altered by some patients' life habits such as alcoholism (Lima et al, 2011), tabagism (Bueno et al, 2011 and coffee consumption (Tsuang et al, 2006;Lacerda et al, 2010). The present study shows that cigarette smoke inhalation and coffee consumption interfered substantially negatively on the bone formation and osseous integration of the DHA implant in the CC group.…”

Section: Discussionmentioning

confidence: 99%

Effects of cigarette smoke inhalation and coffee consumption on bone formation and osseous integration of hydroxyapatite implant

et al. 2013

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The present study aims to assess the effects of cigarette smoke inhalation and/or coffee consumption on bone formation and osseous integration of a dense hydroxyapatite (DHA) implant in rats. For this study, 20 male rats were divided into four groups (n = 5): CT (control) group, CE (coffee) group, CI (cigarette) group and CC (coffee + cigarette) group. During 16 weeks, animals in the CI group were exposed to cigarette smoke inhalation equivalent to 6 cigarettes per day; specimens in the CE group drank coffee as liquid diet; and rats in the CC group were submitted to both substances. In the 6 th week a 5 mm slit in the parietal bone and a 4 mm slit in the tibia were performed on the left side: the former was left open while the latter received a DHA implant. As soon as surgeries were finished, the animals returned to their original protocols and after 10 weeks of exposure they were euthanised (ethically sacrificed) and the mentioned bones collected for histological processing. Data showed that exposure to cigarette smoke inhalation and coffee consumption did not interfere in weight gain and that solid and liquid diet consumption was satisfactory. Rats in the CC group showed a decrease in bone neoformation around the tibial DHA implant (31.8 ± 2.8) as well as in bone formation in the parietal slit (28.6 ± 2.2). On their own, cigarette smoke inhalation or coffee consumption also led to diminished bone neoformation around the implant and delayed the bone repair process in relation to the CT group. However, reduction in the bone repair process was accentuated with exposure to both cigarette smoke inhalation and coffee consumption in this study.Keywords: cigarette, bone, hydroxyapatite, bone formation, coffee.Os efeitos do cigarro e do consumo de café sobre a formação óssea e a integração óssea de implantes de hidroxiapatita ResumoO presente estudo teve como objetivo avaliar os efeitos do tabagismo e do consumo de café, isolada ou concomitantemente, sobre a formação óssea e a osseointegração de implantes hidroxiapatita densa. Foram utilizados 20 ratos machos, divididos em quatro grupos (n = 5): grupo CT (controle); grupo CA (café); grupo CI (cigarro), e grupo CC (cigarro + café). Durante 16 semanas, os animais do grupo CI foram expostos à fumaça de seis cigarros/dia; os animais do grupo CA consumiram café como dieta líquida, e os animais do grupo CC, ambas as substâncias. Após seis semanas de exposição, uma falha óssea de 5 mm foi produzida no osso parietal esquerdo e de 4 mm, na tíbia esquerda dos animais. A falha do parietal foi mantida aberta, enquanto na tíbia corpos cerâmicos de hidroxiapatita densa (HAD) foram implantados em cavidade produzida cirurgicamente. Após as cirurgias, os animais retornaram aos protocolos experimentais e, ao término de dez semanas, foram eutanasiados, sendo as tíbias e os parietais coletados para processamento histológico. A exposição à fumaça do cigarro e o consumo de café não interferiram no ganho de peso dos animais, e os consumos de dieta líquida e sólida foram satisfatórios...

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“…A large body of evidence suggests that a correlation between low bone mass and chronic alcohol abuse [1] with increased fracture risk [2]. Animal studies showed that a alcohol consumption could decrease the new bone formation [3] and delay implant osteointegration [4] possibly by reducing the number and activity of osteoblasts [5]. The detrimental effect of alcohol was even more harmful in comparison with nicotine [6].…”

Section: Introductionmentioning

confidence: 99%

Heterodimeric BMP-2/7 Antagonizes the Inhibition of All-Trans Retinoic Acid and Promotes the Osteoblastogenesis

Zheng

et al. 2013

PLoS ONE

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ObjectivesHypervitaminosis A and alcoholism can result in a low mineral density and compromised regenerative capacity of bone, thus delaying implant osteointegration. The inhibitory effect of all-trans retinoic acid on osteoblastogenesis is considered to be one of the mechanisms. We hypothesized that heterodimeric bone morphogenetic protein-2/7 could antagonize all-trans retinoic acid and enhance osteoblastogenesis, with an aim to accelerate and enhance bone regeneration and implant osteointegration.Materials and MethodsWe applied 5 ng/ml or 50 ng/ml bone morphogenetic protein-2/7 to restore the osteoblastogenesis of pre-osteoblasts (MC3T3-E1 cell line) that was inhibited by 1 µM all-trans retinoic acid. We evaluated the efficacy by assessing cell numbers (proliferation), alkaline phosphatase activity (a marker for early differentiation), osteocalcin (a marker for late differentiation), calcium deposition (a marker for final mineralization) and the expression of osteoblastogenic genes (such as Runx2, Collagen Ia, alkaline phosphatase and osteocalcin) at different time points.ResultsAll-trans retinoic acid significantly inhibited the expression of all the tested osteoblastogenic genes and proteins except alkaline phosphatase activity. In the presence of ATRA, 50 ng/ml bone morphogenetic protein-2/7 not only completely restored but also significantly enhanced all the osteoblastogenic genes and proteins. On the 28th day, mineralization was completely inhibited by all-trans retinoic acid. In contrast, 50 ng/ml BMP-2/7 could antagonize ATRA and significantly enhance the mineralization about 2.5 folds in comparison with the control treatment (no ATRA, no BMP2/7).ConclusionsHeterodimeric bone morphogenetic protein-2/7 bears a promising application potential to significantly promote bone regeneration and implant osteointegration for the patients with hypervitaminosis A and alcoholism.

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Effects of ethanol on the osteogenesis around porous hydroxyapatite implants

Cited by 16 publications

References 18 publications

The effects of binge-pattern alcohol consumption on orthodontic tooth movement

The effects of binge-pattern alcohol consumption on orthodontic tooth movement

Effects of cigarette smoke inhalation and coffee consumption on bone formation and osseous integration of hydroxyapatite implant

Heterodimeric BMP-2/7 Antagonizes the Inhibition of All-Trans Retinoic Acid and Promotes the Osteoblastogenesis

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