Antimicrobial potential of some plant extracts against Candida species

Höfling, José Francisco; Aníbal, Paula Cristina; Obando-Pereda, Gustavo Alberto; Peixoto, I A T; Furletti, V. F.; Foglio, Mary Ann; Gonçalves, Raquel

doi:10.1590/s1519-69842010000500022

Cited by 103 publications

(76 citation statements)

References 25 publications

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“…Other studies in the literature have reported on the antifungal effects of S. cumini on C. albicans (Oliveira et al 2007;H€ ofling et al 2010;Santos et al 2012), but have not further described its mode of action and effects on biofilm morphology and viability (see further in the discussion). As to the chemical profile, the major compounds found in the extract of the leaves in this study were saponins.…”

Section: Discussionmentioning

confidence: 99%

Antifungal potential ofSideroxylon obtusifoliumandSyzygium cuminiand their mode of action againstCandida albicans

Pereira

Freires

Castilho

et al. 2016

Pharmaceutical Biology

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Context The emergence of resistant pathogens and toxicity of antifungals have encouraged an active search for novel candidates to manage Candida biofilms. Objective In this study, the little known species Sideroxylon obtusifolium T.D. Penn (Sapotacea) and Syzygium cumini (L.) Skeels (Myrtaceae), from the Caatinga biome in Brazil were chemically characterized and explored for their antifungal potential against C. albicans. Materials and methods We determined the effects of hydroalcoholic extracts/fractions upon fungal growth (minimum inhibitory and fungicidal concentrations, MIC/MFC), biofilm morphology (scanning electron microscopy) and viability (confocal laser scanning microscopy), proposed their mode of action (sorbitol and ergosterol assays), and finally investigated their effects against macrophage and keratinocyte cells in a cell-based assay. Data were analysed using one-way analysis of variance with Tukey-Kramer post-test (a ¼ 0.05). Results The n-butanol (Nb) fraction from S. obtusifolium and S. cumini extract (Sc) showed flavonoids (39.11 6 6.62 mg/g) and saponins (820.35 6 225.38 mg/g), respectively, in their chemical composition and demonstrated antifungal activity, with MICs of 62.5 and 125 lg/mL, respectively. Nb and Sc may complex with ergosterol as there was a 4-16-fold increase in MICs in the presence of exogenous ergosterol, leading to disrupted permeability of cell membrane. Deleterious effects were observed on morphology and viability of treated biofilms from concentrations as low as their MICs and higher. Sc was not toxic to macrophages and keratinocytes at these concentrations (p > 0.05), unlike Nb. Conclusions Nb and Sc demonstrated considerable antifungal activity and should be further investigated as potential alternative candidates to treat Candida biofilms. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Antifungal potential ofSideroxylon obtusifoliumandSyzygium cuminiand their mode of action againstCandida albicans

Pereira

Freires

Castilho

et al. 2016

Pharmaceutical Biology

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“…Many studies are being carried out to find plant species with potential antifungal activity Höfling et al, 2010), because synthetic drugs may exhibit high toxicity to patients (Araújo et al, 2004). Substances more efficient against pathogenic microorganisms and less toxic to the patient could be isolated from vegetal species (Ostrosky, 2009).…”

Section: Introductionmentioning

confidence: 99%

Vernonanthura polyanthes leaves aqueous extract enhances doxorubicin genotoxicity in somatic cells of Drosophila melanogaster and presents no antifungal activity against Candida spp.

et al. 2016

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Vernonanthura polyanthes (Spreng.) A.J. Vega & Dematt. (Asteraceae), known as "assa-peixe", has been used in ethnomedicine for the treatment of various diseases such as bronchitis, pneumonia, hemoptysis, persistent cough, internal abscesses, gastric and kidney stone pain. Moreover, some studies demonstrated that species of Genus Vernonia present antifungal activity. Due to the biological relevance of this species, the aim of this study was to investigate the toxic, genotoxic, antigenotoxic and antifungal potential of V. polyanthes leaves aqueous extract in somatic cells of Drosophila melanogaster or against Candida spp. The aqueous extract of the plant showed no toxic, genotoxic and antigenotoxic activity in the experimental conditions tested using the wing somatic mutation and recombination test (SMART/wing). However, when the extract was associated with doxorubicin, used in this work as a positive control, the mutagenic potential of doxorubicin was enhanced, increasing the number of mutations in D. melanogaster somatic cells. In the other hand, no inhibitory activity against Candida spp. was observed for V. polyanthes leaves aqueous extract using agar-well diffusion assay. More studies are necessary to reveal the components present in the V. polyanthes leaves aqueous extract that could contribute to potentiate the doxorubicin genotoxicity.Keywords: assa-peixe, Asteraceae, Cerrado, SMART/wing test, Vernonia polyanthes.O extrato aquoso das folhas de Vernonanthura polyanthes potencializa a genotoxicidade da doxorrubicina em células somáticas de Drosophila melanogaster e não apresenta atividade antifúngica contra Candida spp. ResumoVernonanthura polyanthes (Spreng.) A.J. Vega & Dematt. (Asteraceae), conhecida como "assa-peixe", tem sido utilizada na medicina popular para o tratamento de várias doenças, como bronquite, pneumonia, hemoptise, tosse persistente, abcessos internos, afecções gástricas e cálculo renal. Além disso, alguns estudos já demonstraram que espécies do Gênero Vernonia apresentam atividade antifúngica. Devido à relevância biológica dessa espécie, o objetivo deste estudo foi investigar os efeitos citotóxico, genotóxico, antigenotóxico e antifúngico do extrato aquoso das folhas de V. polyanthes em células somáticas de Drosophila melanogaster ou contra Candida spp. O extrato aquoso da planta não apresentou atividade citotóxica, genotóxica e antigenotóxica nas condições experimentais testadas usando o teste de recombinação e mutação somática em asa (SMART-asa). No entanto, quando o extrato foi associado com a doxorrubicina, utilizada neste trabalho como controle positivo, o potencial mutagênico da doxorrubicina foi potencializado, aumentando oBraz.

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“…B. Verl (Bignoniaceae) leaf extract has been used as an infusion to treat diseases such as gastric ulcers, intestinal colic, diarrhea, inflammation, infections, and anemia (Siraichi et al, 2013). The antioxidant (Siraichi et al, 2013), liver protective (Lima de Medeiros et al, 2011), and antimicrobial activity (Hofling et al, 2010) of this plant has been reported recently. Antimicrobial activity of hydroethanol extract of A. chica leaves was tested by the broth microdilution method using a panel of bacteria and yeast of clinical interest.…”

Section: Arrabidaea Chicamentioning

confidence: 99%

Medicinal plants in the treatment ofHelicobacter pyloriinfections

Safavi

Shams-Ardakani

Foroumadi

2014

Pharmaceutical Biology

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Context: Helicobacter pylori is a small, spiral, Gram-negative bacillus that plays a role in the pathogenesis of a number of diseases ranging from asymptomatic gastritis to gastric cancer. Schedule compliance, antibiotic drug resistance, and side-effects of triple or quadruple therapy have led to research for novel candidates from plants.Objective: The purpose of this paper is to review the most potent medicinal plants of recently published literature with anti-H. pylori activity. For centuries, herbals have been used by traditional healers around the world to treat various gastrointestinal tract disorders such as dyspepsia, gastritis, and peptic ulcer disease. The mechanism of action by which these botanicals exert their therapeutic properties has not been completely and clearly elucidated. Anti-H. pylori properties may be one of the possible mechanisms by which gastroprotective herbs treat gastrointestinal tract disorders. Materials and methods: Electronic databases such as PubMed, Google scholar, EBSCO, and local databases were explored for medicinal plants with anti-H. pylori properties between 1984 and 2013 using key words ''medicinal plants'' and ''Helicobacter pylori'' or ''anti-Helicobacter pylori''. Results: A total of 43 medicinal plant species belonging to 27 families including Amaryllidaceae,

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Antimicrobial potential of some plant extracts against Candida species

Cited by 103 publications

References 25 publications

Antifungal potential ofSideroxylon obtusifoliumandSyzygium cuminiand their mode of action againstCandida albicans

Antifungal potential ofSideroxylon obtusifoliumandSyzygium cuminiand their mode of action againstCandida albicans

Vernonanthura polyanthes leaves aqueous extract enhances doxorubicin genotoxicity in somatic cells of Drosophila melanogaster and presents no antifungal activity against Candida spp.

Medicinal plants in the treatment ofHelicobacter pyloriinfections

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