Immunocytochemical study of gastrintestinal endocrine cells in insectivorous bats (Mammalia: Chiroptera)

Cm, Santos; Aa, Nascimento; Al, Peracchi; Sales, Armando; Js, Mikalauskas; Sf, Gouveia

doi:10.1590/s1519-69842008000300026

Cited by 10 publications

(7 citation statements)

References 19 publications

(19 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Bat intestine sections were used as positive controls, because they presented positive responses to these reactions in previous studies (e.g., Santos et al 2008aSantos et al , 2008bMachado-Santos et al 2009). The negative control was prepared by replacing the primary antibody with non-immune serum and PBS (pH 7.4).…”

Section: Immunohistochemical Studymentioning

confidence: 99%

Immunohistochemical study of the six types of endocrine cells in the enteropancreatic system of the lizard Tropidurus torquatus (Squamata: Tropiduridae)

Firmiano

Cardoso

Sales

et al. 2017

The European Zoological Journal

Self Cite

View full text Add to dashboard Cite

The present study investigates the aspects of endocrine cells secreting cholecystokinin, gastrin, insulin, glucagon, somatostatin and serotonin in the enteropancreatic system of Tropidurus torquatus. The specimens were collected in Marambaia Island, Sepetiba Bay, Rio de Janeiro State, Brazil. The animals' intestine and pancreas were removed, fixed, processed and then subjected to immunohistochemical techniques. Three cell types, immunoreactive (IR) to gastrin, cholecystokinin-8 and serotonin, were identified in the intestinal mucosa. Serotonin was found in the small and large intestines, whereas gastrin and cholecystokinin-8 cells were only observed in the large intestine of T. torquatus. Glucagon, somatostatin and insulin were not observed in the intestines at all. Only glucagon, somatostatin and insulin were identified in the pancreas of the studied lizards. The dorsal lobe showed a large number of endocrine cells scattered throughout the exocrine parenchyma and in the exocrine duct walls. The present study shows that the intestine and pancreas of T. torquatus hold most of the regulatory peptides, presenting a structure similar to that in other vertebrates such as birds and mammals. The endocrine cells identified in the lizards' enteropancreatic system evidences the good phylogenetic preservation of the regulatory peptides' molecular structure.

show abstract

Section: Immunohistochemical Studymentioning

confidence: 99%

Immunohistochemical study of the six types of endocrine cells in the enteropancreatic system of the lizard Tropidurus torquatus (Squamata: Tropiduridae)

Firmiano

Cardoso

Sales

et al. 2017

The European Zoological Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…Studies of the endocrine cells in the stomach and gut of bats confirm these adaptations observed in species that have different types of diet [10,29,42].…”

Section: Introductionmentioning

confidence: 61%

What difference exists in the pancreas of mammals with sanguivorous diet? A morphological, stereological and immunohistochemical study of the pancreatic islets of the hematophagous bat Diphylla ecaudata

Machado-Santos¹,

Aquino²,

Mikalauka³

et al. 2013

Regulatory Peptides

View full text Add to dashboard Cite

“…Glucagon‐like peptide 1‐positive cells were found in the lamina proprias and muscular layers of the stomachs, small intestines and large intestines of R. nigromaculata , which seems to be a special case. Many studies have not found that GLP‐1‐positive cells, 5‐HT‐secreting cells, gastrin‐secreting cells, somatostatin‐secreting cells, CCK‐secreting cells and gastric inhibitory peptide‐secreting cells are distributed in the lamina proprias and muscular layers of digestive tracts of animals (Çınar et al, ; Pirone et al, ; Santos et al, ). Some literatures propose that endocrine cells in the digestive tract come from pluripotent stem cells in small intestinal crypts (Nikoulina et al, ; Schonhoff, Giel‐Moloney, & Leiter, ), but that pluripotent stem cells transfer from small intestinal crypts to submucosa and muscularis mucosae have not been reported.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical distribution of glucagon‐like peptide 1 in the digestive system of different aquatic vertebrates

Chu

Ying

et al. 2019

Anat Histol Embryol

View full text Add to dashboard Cite

The distribution of glucagon‐like peptide 1 (GLP‐1)‐positive cells in digestive tracts and pancreases of aquatic vertebrates was investigated by immunohistochemical staining method. The results suggested that GLP‐1‐positive cells were distributed in the columnar mucous epithelium and tubular glands of lamina propria in the digestive system. However, GLP‐1‐positive cells were also found in subepithelial lamina propria of the mucosae and muscularis in each segment of the digestive tract of Rana nigromaculata. The distribution densities of these cells reached peaks in the stomachs, and the middle or end segments of small intestines of Chinese softshell turtle, Bufo gargarizans, R. nigromaculata and catfish, and there was the third distribution density peak in the rectum of catfish. The total amount or overall density of GLP‐1‐positive cells varied a lot in the digestive tracts of different animal species. The distribution density was relatively low in the digestive tract of chub and reached the maximum in the digestive tracts of snakehead and catfish, but no GLP‐1‐positive cells were found in the digestive tract of bighead carp. GLP‐1‐positive cells were densely distributed in the pancreases of Chinese softshell turtle, B. gargarizans and R. nigromaculata. These cells spread over the superficial layers of islets or scattered in exocrine pancreas in the pancreas of B. gargarizans, spread in the endocrine cells or scattered in the pancreas of Chinese softshell turtle, scattered in the pancreas of R. nigromaculata and distributed in the superficial layers of islets in the pancreas of catfish.

show abstract

Immunocytochemical study of gastrintestinal endocrine cells in insectivorous bats (Mammalia: Chiroptera)

Cited by 10 publications

References 19 publications

Immunohistochemical study of the six types of endocrine cells in the enteropancreatic system of the lizard Tropidurus torquatus (Squamata: Tropiduridae)

Immunohistochemical study of the six types of endocrine cells in the enteropancreatic system of the lizard Tropidurus torquatus (Squamata: Tropiduridae)

What difference exists in the pancreas of mammals with sanguivorous diet? A morphological, stereological and immunohistochemical study of the pancreatic islets of the hematophagous bat Diphylla ecaudata

Immunohistochemical distribution of glucagon‐like peptide 1 in the digestive system of different aquatic vertebrates

Contact Info

Product

Resources

About