Ecological roles of natural products of the Brazilian red seaweed Laurencia obtusa

Pereira, Renato Crespo; Gama, Bernardo A.P. da; Teixeira, Valéria Laneuville; Yoneshigue‐Valentin, Yocie

doi:10.1590/s1519-69842003000400013

Cited by 78 publications

(73 citation statements)

References 31 publications

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“…Their constituents range from terpene hydrocarbons, simple and terpene alcohols, aldehydes, ketones, phenols, lactones, coumarins, to sulfur compounds. Yet the phytochemicals citronellol, linalool, and thymol (for having recognized antimicrobial activities) all stand out (Pereira et al, 2003;Priestley et al, 2003;Botelho et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of cariogenic microorganisms to phytoconstituents

et al. 2018

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This study aimed to evaluate the in vitro antibacterial activity of the phytochemicals thymol, linalool, and citronellol against Streptococcus mutans, Streptococcus salivarius and Streptococcus oralis. Disk diffusion screening on solid medium and measurement of the diameter of the bacterial growth inhibition halos was the technique utilized. The Minimum Inhibitory Concentration (MIC) of the substances was determined using serial substance dilutions and microdilution technique in Brain Heart Infusion culture medium. After incubation for 24 hours in an oven at 37 °C, plate reading was completed and confirmed by visual method using 2,3,5 triphenyl tetrazolium chloride dye. The Minimum Bactericidal Concentration (MBC) was determined from MIC subcultures. Assays were performed in triplicate, and chlorhexidine was used as a positive control. The diameters in mm of the growth inhibition halos ranged between 7.3 and 10.7 for S. mutans, 7.3 and 10.0 for S. oralis, and 8.2 and 9.8 for S. salivarius. The MIC and MBC values obtained converged , ranging from maximum values in the presence of Linalool (1,250.0 mg/mL, 2,500.0 mg/mL and 2,500.0 mg/mL, respectively, for S. mutans, S. oralis, and S. salivarius); and minimum values with Thymol (312.5 μg/ml, 156.2 μg/mL and 156.2 μg/ml, respectively for S. mutans, S. oralis, and S. salivarius). All the tested phytochemicals displayed antibacterial activity, thus representing substances with potential applications in preventing tooth decay.Keywords: preventive dentistry, dental caries, citronellol, linalool, thymol. Susceptibilidade de microrganismos cariogênicos a fitoconstituintes ResumoEste estudo objetivou avaliar a atividade antibacteriana in vitro dos fitoquímicos timol, linalol e citronelol sobre Streptococcus mutans, Streptococcus salivaris e Streptococcus oralis. Utilizou-se a técnica de discos de difusão em meio sólido e medição do diâmetro dos halos de inibição. A concentração inibitória mínima (CIM) das substâncias foi determinada utilizando diluições em série das substâncias e técnica de microdiluição em meio de cultura de Brain Heart Infusion. Após incubação durante 24 horas em estufa a 37 °C, a leitura da placa foi confirmada pelo método visual usando o corante 2,3,5 trifenil cloreto de tetrazólio. A concentração bactericida mínima (CBM) foi determinada a partir de subculturas de MIC. Os ensaios foram realizados em triplicata, e clorexidina foi usada como um controle positivo. Os diâmetros dos halos de inibição do crescimento variaram entre 7,3 e 10,7 por S. mutans, 7,3 e 10,0 por S. oralis, e 8,2 e 9,8 para S. salivaris. Os valores de CIM e CBM obtidos variaram de valores máximos na presença de linalol (1.250,0 mg/mL, 2.500.0 mg/mL e 2.500.0 mg/mL, respectivamente, para o S. mutans, S oralis e S. salivaris); a valores mínimos com timol (312,5 μg/ml, 156,2 μg/mL e 156,2 μg

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Section: Introductionmentioning

confidence: 99%

Susceptibility of cariogenic microorganisms to phytoconstituents

et al. 2018

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“…In general, algae synthesize lipophilic non-polar secondary metabolites such as terpenoids and acetogeninas as compounds of mixed biosynthesis occur in low concentrations. Polar polyphenols may occur in high concentrations (Pereira et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Antinociceptive activities of crude methanolic extract and phases, n-butanolic, chloroformic and ethyl acetate from Caulerpa racemosa (Caulerpaceae)

Souza¹,

Queiroz²,

Miranda³

et al. 2009

Rev. bras. farmacogn.

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RESUMO: "Atividade antinociceptiva do extrato metanólico bruto e das fases n-butanólica, clorofórmica e acetato de etila de Caulerpa recemosa (Caulerpaceae)". Neste estudo, tentamos identificar a atividade antinociceptiva do extrato metanólico bruto e das fases n-butanólica, clorofórmica e acetato de etila provenientes da alga Caulerpa racemosa. Esta alga é cosmopolita no mundo, principalmente em regiões tropicais. O extrato metanólico bruto e as fases n-butanólica, clorofórmica e acetato de etila foram administrados por via oral, na concentração de 100 mg/ kg. Estes foram capazes de reduzir a nocicepção produzida pelo ácido acético, sendo 47,39%, 70,51%, 76,11% e 72,24%, respectivamente. No ensaio da placa quente as fases clorofórmica e acetato de etila foram ativas neste modelo. Na fase neurogênica do teste de formalina, foi observado que o extrato metanólico bruto (51,77%), fase n-butanólica (35,12%), fase clorofórmica (32,70%) e indometacina (32,06%) foram eficazes em inibir a resposta nociceptiva. Na fase inflamatória, apenas a fase acetato de etila (75,43%) e indometacina (47,83%) foram capazes de inibir significativamente a resposta nociceptiva. Com base nestes dados, podemos sugerir que o a fase acetato de etila apresenta um significativo efeito anti-inflamatório, cuja potência ainda não foi determinada. No entanto, estudos farmacológicos e químicos serão necessários, a fim de caracterizar o mecanismo responsável pela ação antinociceptiva e também para identificar outros princípios ativos presentes na alga Caulerpa racemosa.Unitermos: Caulerpa racemosa, Caulerpaceae, antinociceptivo, anti-inflamatório, alga. ABSTRACT:In this study, we attempted to identify the possible antinociceptive actions of n-butanolic phase, chloroformic phase, ethyl acetate phase and crude methanolic extract obtained from Caulerpa racemosa. This seaweed is cosmopolitan in world, mainly in tropical regions. The n-butanolic, chloroformic, ethyl acetate phases and crude methanolic extract, all administered orally in the concentration of 100 mg/kg, reduced the nociception produced by acetic acid by 47.39%, 70.51%, 76.11% and 72.24%, respectively. In the hotplate test the chloroformic and ethyl acetate phase were activite in this models. In the neurogenic phase on formalin test, were observed that crude methanolic extract (51.77%), n-butanolic phase (35.12%), chloroformic phase (32.70%) and indomethacin (32.06%) were effective in inhibit the nociceptive response. In the inflammatory phase, only the ethyl acetate phase (75.43%) and indomethacin (47.83%) inhibited significantly the nociceptive response. Based on these data, we can infer that the ethyl acetate phase shows a significant anti-inflammatory profile, whose power has not yet been determined. However, pharmacological and chemical studies are continuing in order to characterize the mechanism(s) responsible for the antinociceptive action and also to identify other active principles present in Caulerpa racemosa.

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“…Red algae of the genus Laurencia J. V. Lamour (Rhodomelaceae) are found in tropical and subtropical regions throughout the world and are an extremely rich source of secondary metabolites with diverse structural features, mainly halogenated sesquiterpenes and C15-acetogenins [7][8] . It is suggested that these metabolites function as a chemical defense against herbivores 9 , fouling organisms, and pathogens 10 . These compounds have been shown to possess some interesting pharmacological activities, such as antitumor 11 , antibacterial 12 , antifungal 13 , and antiviral activities 14 .…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Studies on Laurencia Obtusa (Hudson) Lamourux

Antonisamy

Babu

Janakiraman

et al. 2012

Int J of Biomed & Adv Res

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Aim: The present study was aimed to explore the phytochemical constituents present in Laurencia obtusa collected from Tamil Nadu, South India. Methods: Phytochemical screening of different extracts was carried out using the standard procedure and the results were illustrated. Results: Steroids show the maximum (4/6) presence in acetone, benzene, chloroform and petroleum ether extracts followed by alkaloids (3/6) in ethanol, acetone and chloroform extracts. The UV-Vis phytochemical profile of various extracts of L. obtusa was tabulated. The qualitative HPLC fingerprint profile of ethanolic extract of L. obtusa was selected at a wavelength of 254 nm due to sharpness of the peaks and proper baseline. The profile displayed one prominent peak at a retention time of 1.953 min and some moderate peaks were observed at a retention time of 3.000, 2.570 and 2.467 min respectively. Conclusion:The present studies on L. obtusa produced novel phytochemical markers in standardization as useful analytical tools to check not only the quality of the powder but also the presence of adulterants in ayurvedic drugs.

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Ecological roles of natural products of the Brazilian red seaweed Laurencia obtusa

Cited by 78 publications

References 31 publications

Susceptibility of cariogenic microorganisms to phytoconstituents

Susceptibility of cariogenic microorganisms to phytoconstituents

Antinociceptive activities of crude methanolic extract and phases, n-butanolic, chloroformic and ethyl acetate from Caulerpa racemosa (Caulerpaceae)

Phytochemical Studies on Laurencia Obtusa (Hudson) Lamourux

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