Riesgo de transmisión de Trypanosoma cruzi en el municipio de Metztitlán, estado de Hidalgo, México, mediante la caracterización de unidades domiciliares y sus índices entomológicos

Flores, Marco Antonio Becerril; Angeles-Pérez, Vidal; Noguéz-García, Julio; Palafox, José Luis Imbert

doi:10.1590/s1519-566x2010000500021

Cited by 16 publications

(12 citation statements)

References 13 publications

(21 reference statements)

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“…During the triatomine sampling, a survey was conducted to identify the risk factors associated with triatomine presence in houses and the transmission of T. cruzi [15-17]. This was performed in 25 houses in the Gumake community because this community was easier to access.…”

Section: Methodsmentioning

confidence: 99%

Genotyping of Trypanosoma cruzi in a hyper-endemic area of Colombia reveals an overlap among domestic and sylvatic cycles of Chagas disease

et al. 2014

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BackgroundChagas disease is a neglected illness caused by the Trypanosoma cruzi parasite, which widely affects American communities. This study attempted to identify T. cruzi genotypes circulating in four indigenous communities of the Sierra Nevada of Santa Marta, Colombia, to investigate parasite transmission dynamics in these communities. In addition, some epidemiological variables to determine the risk factors for infection with this parasite, such as the prevalence of T. cruzi infection, the triatomine species, and the domestic and sylvatic mammals that act as vectors and reservoirs of the parasite in the domestic, peridomestic and sylvatic cycles, were examined.MethodsWe developed a prospective study to identify the main risk factors associated with T. cruzi infection in the region. The T. cruzi prevalence was determined by ELISA, IFA and PCR. Triatomines species and both domestic and sylvatic mammals from all communities were captured and sampled. To analyze parasite transmission dynamics in these four communities, eight DNA parasite probes were generated from insect and reservoir samples, and a DNA blot analysis were carried out.ResultsSerological studies revealed 37% prevalence in the four communities, and Kasakumake was the most endemic region, containing approximately 70% seropositives. Moreover, the molecular diagnosis showed a high correlation between the serological data and the T. cruzi circulating in the patients’ blood. A total of 464 triatomine insects were collected in domestic, peridomestic and sylvatic environments, and these insects belonged to five different species; Rhodnius prolixus and Triatoma dimidiata were the two more important species transmitting the parasite. After studying the eco-epidemiological factors in these four communities, the most important risk factors for infection with the parasite were determined. These risk factors are a high infection rate of people and domestic animals, the construction materials of the houses, the presence of infected triatomines inside the human dwellings, the proximity between houses and a sylvatic environment with several triatomine species and wild animals. Finally, the molecular characterization of T. cruzi showed the presence of three haplotypes and complex T. cruzi mixed infections in all reservoirs.ConclusionsActive transmission of T. cruzi is present in four indigenous communities of the Sierra Nevada de Santa Marta with overlap between the domestic and the sylvatic transmission cycles of Chagas disease.

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Section: Methodsmentioning

confidence: 99%

Genotyping of Trypanosoma cruzi in a hyper-endemic area of Colombia reveals an overlap among domestic and sylvatic cycles of Chagas disease

et al. 2014

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“…The presence of synanthropic rodents infected with T. cruzi does not imply that the population is going to be irremediably infected; however, these rodents represent a potential risk to public health, since the combination of other factors such as an increase in density of the population of rodents and vectors, the growth of urbanization and agricultural activity, and the presence of more than one reservoir could favor a greater dissemination of the disease in rural communities [ 2 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, many vectors could increase the probability of infection in rodents. Other factors that could contribute to this positivity in the samples could be the number of reservoirs, the density of the population of vectors, the density of the susceptible population, or the existence of programs of control of vectors in the regions, and the capacity of vector to adapt to humans habitats [ 2 , 20 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Frequency of Trypanosoma cruzi Infection in Synanthropic and Wild Rodents Captured in a Rural Community in Southeast of Mexico

Hernández-Cortazar

Guardia

Torres–Castro

et al. 2018

Veterinary Medicine International

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The protozoan parasite Trypanosoma cruzi is the causative agent of the Chagas disease, which is endemic in southeastern Mexico and is transmitted by the vector Triatoma dimidiata (triatomide). T. cruzi infect a great variety of domestic and wild mammals; rodents are considered one of the most important reservoirs of the parasite in the transmission cycles of T. cruzi. The objective of this study was to determine the frequency of T. cruzi infection and to determine the parasitic load in synanthropic and wild rodents from the rural community of southern Mexico. A total of 41 blood samples and 68 heart tissue samples were collected from various species of synanthropic (n= 48 in 2 species) and wild rodents (n= 35 in 5 species). DNA was extracted from samples to detect the presence of T. cruzi through quantitative PCR (qPCR). T. cruzi DNA was detected in the 9.75% of the blood samples of the synanthropic species (4/41) (14.28%) for Rattus rattus samples and 25% for Ototylomys phyllotis samples, with an average of parasitic load of 4.80 ± 1.17 parasites/μL. In the case of heart tissue samples, 10.29% were positive for T. cruzi (7/68) (8.7% for Rattus rattus, 40% for Peromyscus yucatanicus, and 42.8% for Ototylomys phyllotis) with an average parasite load of 3.15 ± 1.98 eq-parasites/mg. The active and chronic infection of T. cruzi in synanthropic or wild rodents of the rural community of southern Mexico evidences the natural infection in these reservoirs which contribute to maintaining the agent in the wild and domestic environments and can represent a risk of infection for the human population when the vector is present.

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“…De ahí que futuros estudios deberían determinar que marcadores son los más adecuados para hacer más sensible y confiable la técnica de PCR para la detección de infecciones por T. cruzi en insectos vectores. El promedio de chinches infectadas encontrado por el método microscópico en este estudio en el municipio de Metztitlán (20%) es aparentemente mucho mayor que lo reportado en un estudio previo para el mismo municipio (7.7%) (Becerril et al, 2010;Castro-Tenorio et al, 2017). Sin embargo, en el presente reporte sólo se analizaron 5 chinches en Metztitlán, y la diferencia en la prevalencia de infección por T. cruzi en las chinches estudiadas en ese municipio, podría tener más que ver con un problema del tamaño de muestra que con una diferencia real en prevalencia.…”

Section: Dimidiataunclassified

Caracterización molecular de aislados de Trypanosoma cruzi de triatominos recolectados en los municipios del Estado de Hidalgo, México

España

Evangelista

Vázquez-Chagoyán

et al. 2019

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Introducción: Trypanosoma cruzi (T. cruzi), esta clasificado en seis linajes mediante marcadores moleculares de tipificación, que son fácilmente amplificados por técnicas de biología molecular. El objetivo del trabajo fue identificar y ubicar geográficamente a los aislados de T. cruzi que infectan naturalmente a los triatominas de los municipios del Estado de Hidalgo a través de la técnica de PCR, que amplifica fragmentos de la región intergénica del gen mini-exón. Método: Se recolectaron 170 muestras de insectos hematófagos en 14 municipios del Estado de Hidalgo, México. El diagnóstico de laboratorio en las muestras de heces y de tejido digestivo de los triatominos, se realizó de manera convencional por microscopia óptica y por la técnica de PCR para determinar la presencia o ausencia de T. cruzi y para la identificación del linaje correspondiente del parásito.Resultados: Se identificaron tres taxones de triatominos: Triatoma dimidiata (87/170), Triatoma mexicana (14/170) y Triatoma gerstaeckeri (7/170). En el 36.47% (62/170) de los especímenes colectados la especie no pudo ser identificada y se clasificaron únicamente como T. spp. Se determinó la presencia del parásito en el 1.76% de los vectores analizados por el método parasitoscópico y en el 11.17% por el método de biología molecular. El total de los parásitos analizados corresponde al biotipo TcI de T. cruzi. En el ecotopo peridoméstico, se encontró la mayor abundancia de triatominos (80.58%) y el mayor porcentaje (10.58%) de infección por T. cruzi.Conclusiones: El vector más importante encontrado en la región en estudio fue Triatoma dimidiata seguido de T. mexicana y T. gerstaekeri y el biotipo con el que están mayormente infectados es el TcI. Los triatominos encontrados se distribuían principalmente en hábitats peridomésticos en los municipios estudiados. Los resultados indican la existencia de riesgo de infección para los habitantes de esas regiones endémicas del Estado de Hidalgo, México.

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Riesgo de transmisión de Trypanosoma cruzi en el municipio de Metztitlán, estado de Hidalgo, México, mediante la caracterización de unidades domiciliares y sus índices entomológicos

Cited by 16 publications

References 13 publications

Genotyping of Trypanosoma cruzi in a hyper-endemic area of Colombia reveals an overlap among domestic and sylvatic cycles of Chagas disease

Genotyping of Trypanosoma cruzi in a hyper-endemic area of Colombia reveals an overlap among domestic and sylvatic cycles of Chagas disease

Frequency of Trypanosoma cruzi Infection in Synanthropic and Wild Rodents Captured in a Rural Community in Southeast of Mexico

Caracterización molecular de aislados de Trypanosoma cruzi de triatominos recolectados en los municipios del Estado de Hidalgo, México

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