Using body length measurements to study larval growth: a lepidopteran example

Costa, Felipe A. P. L.; Gomes-Filho, Arlindo

doi:10.1590/s1519-566x2002000200002

Cited by 6 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As lagartas alimentadas com couve tratada foram pesadas em balança de precisão de 0,0001 g, no início e no final do 4° instar, período de maior consumo alimentar pelas lagartas, e medido o comprimento (cm) no final do 4° instar (Bellanda Barros & Zucoloto, 1999;Costa & Gomes-Filho, 2002).…”

Section: Methodsunclassified

Deterrência alimentar em Ascia monuste orseis Godart (Lepidoptera: Pieridae) induzida por soluções homeopáticas

et al. 2015

View full text Add to dashboard Cite

A couve, Brassica oleracea var. acephala, destaca-se entre as plantas hortícolas como sendo frequentemente atacada por pragas, dentre as quais o curuquerê da couve, Ascia monuste orseis (Godart, 1819) (Lepidoptera: Pieridae). O controle desse inseto tem sido feito com inseticidas. Na agricultura orgânica, o uso dos referidos produtos é proibido e já existem alguns casos em que agricultores estão substituindo-o, por outras alternativas menos danosas ao meio ambiente, como as soluções homeopáticas, substâncias apontadas como ferramentas para Agroecologia. Este trabalho teve por objetivo verificar se soluções homeopáticas proporcionam mecanismos de antibiose, como deterrência alimentar, em Ascia monuste orseis, em couve 'manteiga cv. Santo Antônio' e se podem ser utilizadas no controle de pragas. As soluções testadas foram: - Sulphur 12CH; Phosphorus 5CH; Magnesia carbonica 30CH; Ruta 5CH. A testemunha foi água destilada + álcool de cereais 70% 5CH. Para o preparo de cada solução, foram retirados 0,2 ml de cada preparado homeopático, adicionados a 200 ml de água destilada pulverizados nas folhas e nos solo dos vasos. As características analisadas foram peso de lagartas no início e no final do 4° instar; peso seco de pupa (biomassa incorporada), comprimento de lagarta no 4° instar, duração do ciclo ovo-adulto, percentagem de emergência de adultos, comprimento alar, fecundidade das fêmeas e valor nutritivo das couves tratadas. Sulphur 12CH pode ser recomendado como método alternativo eficiente no controle de A. monuste orseis. Todas as soluções homeopáticas, com exceção do Phosphorus 5CH, promoveram deterrência alimentar, mecanismo de antibiose, interferindo no ciclo biológico de A. monuste orseis.

show abstract

Section: Methodsunclassified

Deterrência alimentar em Ascia monuste orseis Godart (Lepidoptera: Pieridae) induzida por soluções homeopáticas

et al. 2015

View full text Add to dashboard Cite

show abstract

“…We deposited these photographs in the Dryad repository (Hu et al, 2022a(Hu et al, , 2022b. For all larvae, a calibration slide was used to measure length, which we used as an approximation of larval age (Costa & Gomes-Filho, 2002;Zeller & Koella, 2016). In C. varians we arbitrarily defined three larval age/length groups for use in statistics.…”

Section: Relative Age Estimates For All Larvae and C Varians Adultsmentioning

confidence: 99%

Partner fidelity and environmental filtering preserve stage‐specific turtle ant gut symbioses for over 40 million years

D’Amelio

Béchade

et al. 2023

Ecological Monographs

View full text Add to dashboard Cite

Sustaining beneficial gut symbioses presents a major challenge for animals, including holometabolous insects. Social insects may meet such challenges through partner fidelity, aided by behavioral symbiont transfer and transgenerational inheritance through colony founders. We address such potential through colony‐wide explorations across 13 eusocial, holometabolous insect species in the ant genus Cephalotes. Through amplicon sequencing, we show that previously characterized worker microbiomes are conserved in soldier castes, that adult microbiomes exhibit trends of phylosymbiosis, and that Cephalotes cospeciate with their most abundant adult‐enriched symbiont. We find, also, that winged queens harbor worker‐like microbiomes prior to colony founding, suggesting vertical inheritance as a means of partner fidelity. Whereas some adult‐abundant symbionts colonize larvae, larval gut microbiomes are uniquely characterized by environmental bacteria from the Enterobacteriales, Lactobacillales, and Actinobacteria. Distributions across Cephalotes larvae suggest more than 40 million years of conserved environmental filtering and, thus, a second sustaining mechanism behind an ancient, developmentally partitioned symbiosis.

show abstract

“…Prior to DNA extraction, all specimens were photographed from the dorsal side by a digital camera connected to a Leica 80x dissecting microscope under constantlighting conditions (Supplementary Figure 1). For all larval specimens, a calibration slide was used to measure length, which we used as an approximation of larval age [77,78]. In Cephalotes varians we arbitrarily defined three age groups for larvae for use in statistics confined to this species.…”

Section: Relative Age Estimates For All Larvae and C Varians Adultsmentioning

confidence: 99%

The secrets to domestic bliss – Partner fidelity and environmental filtering preserve stage-specific turtle ant gut symbioses for over 40 million years

D’Amelio

Béchade

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Gut microbiomes can vary across development, a pattern often found for insects with complete metamorphosis. With varying nutritional need and distinct opportunities for microbial acquisition, questions arise as to how such holometabolous insects retain helpful microbes at larval and adult stages. Ants are an intriguing system for such study. In a number of lineages adults digest only liquid food sources, while larvae digest solid foods. Like some other social insects, workers and soldiers of some ant species engage in oral-anal trophallaxes, enabling microbial transfer among siblings. But do queens, the typical colony founding caste, obtain symbionts through such transfer? Does this enable transgenerational symbiont passage? And does the resulting partner fidelity promote the evolution of beneficial symbionts? Furthermore, how might such adult-centric biology shape larval microbiomes? To address these questions, we characterized symbiotic gut bacteria across 13 species of Cephalotes turtle ants, with up to 40-million years of divergence. Adding to the prior focus on workers we, here, study underexplored castes and stages including queens, soldiers, and larvae, by performing 16S rRNA qPCR, amplicon sequencing, and phylogenetic classification. Results: We show that adult microbiomes are conserved across species and largely across castes. Nearly 95% of the bacteria in adults have, thus far, been found only in Cephalotes ants. Furthermore, the microbiomes from most adults exhibit phylosymbiosis, a trend in which microbiome community similarity recapitulates patterns of host relatedness. Additionally, an abundant, adult-enriched symbiont cospeciates with some Cephalotes. Evidence here suggests that these partner fidelity patterns extend from transgenerational symbiont transfer through alate gyne dispersal and subsequent colony-founding by queens. Like adults, larvae of Cephalotes species exhibit strong microbiome conservation. Phylosymbiosis patterns are weaker, however, with further evidence elevating environmental filtering as a primary mechanism behind such conservation. Specifically, while adult-enriched symbionts are found in most larvae, symbionts of older larvae are highly related to free-living bacteria from the Enterobacteriaceae, Lactobacillales, and Actinobacteria. Conclusions: Our findings suggest that both partner fidelity and conserved environmental filtering drive stable, stage-specific, social insect symbioses. We discuss the implications for our broader understanding of insect microbiomes, and the means of sustaining a beneficial microbiome.

show abstract

Using body length measurements to study larval growth: a lepidopteran example

Cited by 6 publications

References 13 publications

Deterrência alimentar em Ascia monuste orseis Godart (Lepidoptera: Pieridae) induzida por soluções homeopáticas

Deterrência alimentar em Ascia monuste orseis Godart (Lepidoptera: Pieridae) induzida por soluções homeopáticas

Partner fidelity and environmental filtering preserve stage‐specific turtle ant gut symbioses for over 40 million years

The secrets to domestic bliss – Partner fidelity and environmental filtering preserve stage-specific turtle ant gut symbioses for over 40 million years

Contact Info

Product

Resources

About