Detecção de Wolbachia em uma População Telítoca de Trichogramma atopovirilia Oatman &amp; Platner (Hymenoptera: Trichogrammatidae) via PCR com o Primer Específico wsp

Ciociola, Américo I.; Almeida, R. P. de; Zucchi, Roberto A.; Stouthamer, Richard

doi:10.1590/s1519-566x2001000300027

Cited by 13 publications

(4 citation statements)

References 6 publications

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“…Trichogramma wasps manifest a haplodiploid mode of reproduction in which daughters (diploids) arise from fertilized eggs and sons (haploids) from unfertilized eggs. Out of 180 known nominal species (Pinto 1999), Wolbachia is known to induce parthenogenesis in at least 14 Trichogramma species (Stouthamer 1997;Schilthuizen & Stouthamer 1997;Pinto & Stouthamer 1994;Pintureau et al 2000a;Ciociola et al 2001). Females infected with PI Wolbachia produce daughters from both their fertilized and unfertilized eggs.…”

Section: Introductionmentioning

confidence: 99%

Natural interspecific and intraspecific horizontal transfer of parthenogenesis–inducingWolbachiainTrichogrammawasps

Huigens¹,

Almeida

Boons³

et al. 2004

Proc. R. Soc. Lond. B

238

249

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The intracellular bacterium Wolbachia is one of the most common symbionts in arthropods and, because of its manipulative effects on host reproduction, is assumed to be an important factor in several evolutionary processes. These bacteria are mainly vertically transmitted from mother to daughter through the egg cytoplasm, and horizontal transmission is generally assumed to be rare. Here, we show natural inter-and intraspecific horizontal transfer of parthenogenesis-inducing Wolbachia between parasitoid wasps of the genus Trichogramma. Horizontal transfer was observed when infected and uninfected larvae shared the same host egg. This is the first report, to our knowledge, on interspecific horizontal transfer of Wolbachia between closely related sympatric species. Some originally uninfected immature wasps acquired Wolbachia while inside the host egg, but not all of these newly infected females exhibited the parthenogenesis phenotype. In general, intraspecific horizontal transfer was more successful than interspecific transfer. Wolbachia underwent vertical transmission in the new species but the infection tended to be lost within several generations. Our results have important implications for understanding the evolution of Wolbachia-host associations.

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Section: Introductionmentioning

confidence: 99%

Natural interspecific and intraspecific horizontal transfer of parthenogenesis–inducingWolbachiainTrichogrammawasps

Huigens¹,

Almeida

Boons³

et al. 2004

Proc. R. Soc. Lond. B

238

249

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show abstract

“…In conclusion, like other South American arthropods (Shoemaker et al ., 2000; Ciociola Jr et al ., 2001; Ono et al ., 2001; Selivon et al ., 2002; Vega et al ., 2002; Dittmar and Whiting, 2004; Heukelbach et al ., 2004; Rocha et al ., 2005; Cônsoli and Katajima, 2006; Souza et al ., 2009), Wolbachia also infects South American terrestrial isopods. The population-based approach we took allowed a more accurate estimation of prevalence rates in these species, and permitted the discovery of a high genetic diversity of Wolbachia isolates.…”

Section: Discussionmentioning

confidence: 99%

First record of Wolbachia in South American terrestrial isopods: prevalence and diversity in two species of Balloniscus (Crustacea, Oniscidea)

et al. 2012

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Wolbachia are endosymbiotic bacteria that commonly infect arthropods, inducing certain phenotypes in their hosts. So far, no endemic South American species of terrestrial isopods have been investigated for Wolbachia infection. In this work, populations from two species of Balloniscus (B. sellowii and B. glaber) were studied through a diagnostic PCR assay. Fifteen new Wolbachia 16S rDNA sequences were detected. Wolbachia found in both species were generally specific to one population, and five populations hosted two different Wolbachia 16S rDNA sequences. Prevalence was higher in B. glaber than in B. sellowii, but uninfected populations could be found in both species. Wolbachia strains from B. sellowii had a higher genetic variation than those isolated from B. glaber. AMOVA analyses showed that most of the genetic variance was distributed among populations of each species rather than between species, and the phylogenetic analysis suggested that Wolbachia strains from Balloniscus cluster within Supergroup B, but do not form a single monophyletic clade, suggesting multiple infections for this group. Our results highlight the importance of studying Wolbachia prevalence and genetic diversity in Neotropical species and suggest that South American arthropods may harbor a great number of diverse strains, providing an interesting model to investigate the evolution of Wolbachia and its hosts.

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“…Morphological taxonomic confirmation was intensively investigated by Professor Roberto Antonio Zucchi, influenced by Dr. John Pinto (UCR, USA) and Dr. Bernard Pintureau (INRA, France) [54]. Molecular identification techniques were also studied [55], as well as identification by hyperspectral imaging [56].…”

Section: Successful Cases In Brazil Withmentioning

confidence: 99%

Insect Rearing Techniques for Biological Control Programs, a Component of Sustainable Agriculture in Brazil

Parra

Coelho

2022

Insects

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This article describes the importance of rearing insects, whether on a small scale for research or a large scale for mass rearing, for use in biological control (BC) programs with macro-organisms. These inter- or multidisciplinary research programs are necessarily long-term and depend on rearing techniques for their complete development. Some successful examples of BC in Brazil are presented, including case studies of Trichogramma spp. These required broad bioecological studies that provided the basis for both mass rearing and transfer of the necessary technology to farmers. This has allowed Brazil to occupy a leadership position in biological control in “Open Fields”. For example, about three million ha are being treated with Trichogramma galloi (a native parasitoid), and about three and a half million ha with Cotesia flavipes (an exotic parasitoid) to control Diatraea saccharalis, the sugarcane borer. These natural enemies are produced by commercial firms, or by laboratories in sugar and alcohol plants themselves, in the case of C. flavipes.

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Detecção de Wolbachia em uma População Telítoca de Trichogramma atopovirilia Oatman & Platner (Hymenoptera: Trichogrammatidae) via PCR com o Primer Específico wsp

Cited by 13 publications

References 6 publications

Natural interspecific and intraspecific horizontal transfer of parthenogenesis–inducingWolbachiainTrichogrammawasps

Natural interspecific and intraspecific horizontal transfer of parthenogenesis–inducingWolbachiainTrichogrammawasps

First record of Wolbachia in South American terrestrial isopods: prevalence and diversity in two species of Balloniscus (Crustacea, Oniscidea)

Insect Rearing Techniques for Biological Control Programs, a Component of Sustainable Agriculture in Brazil

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