2015
DOI: 10.1590/s1517-838246420140942
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Abstract: Thermophilic 32 isolates and 20 reference bacilli were subjected to Rep-PCR and ITS-PCR fingerprinting for determination of their genotypic diversity, before screening lipase activities. By these methods, all the isolates and references could easily be differentiated up to subspecies level from each other. In screening assay, 11 isolates and 7 references were found to be lipase producing. Their extracellular lipase activities were measured quantitatively by incubating in both tributyrin and olive oil broths at… Show more

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Cited by 7 publications
(5 citation statements)
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References 28 publications
(35 reference statements)
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“…To carry enzymatic reaction the test tubes were inoculated at 60°C for 15 min and the reaction stopped by storing the solution at −20°C for 8 min in order to cool down. The release of pNP (para-nitrophenol) at 400 nm was measured, and one unit of enzyme was defined as the amount of enzyme needed for the hydrolysis of 1 μM p-NPP per minute at 60 °C, pH 7.0 (Koc et al, 2015).…”
Section: β-Glucosidase Activitymentioning
confidence: 99%
“…To carry enzymatic reaction the test tubes were inoculated at 60°C for 15 min and the reaction stopped by storing the solution at −20°C for 8 min in order to cool down. The release of pNP (para-nitrophenol) at 400 nm was measured, and one unit of enzyme was defined as the amount of enzyme needed for the hydrolysis of 1 μM p-NPP per minute at 60 °C, pH 7.0 (Koc et al, 2015).…”
Section: β-Glucosidase Activitymentioning
confidence: 99%
“…Contudo, distintos tempos de incubação têm sido reportados para a máxima produção de xilanases: 24 h para Geobacillus stearothermophilus KIBGE-IB29 (Bibi Z et al, 2014), 56 h para Bacillus pumilus VLK-1 (Kumar L et al, 2014), 96 h para Arthrobacter sp.MTCC 6915 (Murugan S et al, 2011) e Bacillus mojavensis AG137 (Sepahy AA, Ghazi S, Sepahy MA, 2011). ótimo para a produção de lipases por bactérias isoladas do solo (Willerding AL et al, 2011;Sahoo RK, Subudhi E, Kumar M, 2014;Koc M, Cokmus C, Cihan AC, 2015).…”
Section: Discussionunclassified
“…Within the scope of the studies, 20 Geobacillus and 85 Anoxybacillus isolates and reference strains, were used from the culture collection of Ankara University, Biology Department, Microbiology Research Laboratory. The list of isolates and reference strains were as given in Table 1 (Cihan et al, 2011;Koc et al, 2015). All thermophilic bacilli, stored in glycerol stocks at -86°C, were first inoculated on MI (Medium I), starch; 10 g/L, peptone from casein; 5 g/L, yeast extract; 3 g/L, meat extract; 3 g/L, K2HPO4; 3 g/L, KH2PO4; 1g/L, agar 30 g/L, pH: 7.0 ± 0.2) agar for 18 h at 55°C (Suzuki et al, 1976).…”
Section: Bacterial Culturesmentioning
confidence: 99%
“…List of isolates and reference strains used in this study. The origins of the isolates were presented in the previous studies of Cihan et al (2011) and Koc et al (2015) for Geobacillus and Anoxybacillus members, respectively.…”
Section: Bacterial Culturesmentioning
confidence: 99%