Biosurfactant production by &lt;italic&gt;Pseudomonas aeruginosa&lt;/italic&gt;in kefir and fish meal

Kaşkatepe, Banu; Yıldız, Sulhiye; Gümüştaş, Mehmet; Özkan, Síbel A.

doi:10.1590/s1517-838246320140727

Cited by 27 publications

(15 citation statements)

References 23 publications

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“…The E 24 of supernatant was 63% and the surface tension was 26.1 mN/m. Wei it al., (45) found that the best pH range for RL production was 6-6.8, which is similar to observations in this study, while Kaskatepe et al, (18) found the best was 6.8. A study by Moussa et al,(24) concluded that the optimum pH for production of di-RL P. aeruginosa TMN was 7, which is also close to the value obtained in this study.…”

Section: Extraction Of Rlsupporting

confidence: 89%

“…These findings confirm that RL was majorly produced during slowing down of cell growth, while production is limited during the exponential growth phase, which is in agreement with previous conclusion by Maier and Sorbeon-Chavez (21) that this product is a secondary metabolite of P. aeruginosa during the stationary phase of growth. A study by Kaskatepe et al,(18) shown that 7 days was the optimum incubation period, while Twigg et al, (44) obtained maximum amount after 72 h Figure 7. Effect of incubation period on RL production by P. aeruginosa A3 in batch culture TLC After purification of crude RL by column chromatography, the separated fractions were analyzed using TLC.…”

Section: Extraction Of Rlmentioning

confidence: 94%

“…Figure 5, Rhamnolipid production was increased as the temperature raise from 25ºC to 30ºC, remained constant at 34ºC, a slight decrease in production observed at 37ºC, then a sharp decrease appeared as the temperature shifted to 40ºC, so the optimum temperature range for biosurfactant production by P. aeruginosa A3 was in the range 30-34ºC when the surface tension was 26 mN/m and the E 24 was 63%. These observations were in agreement with a study conducted by Wei it al., (45), while the optimum temperature for production of RL by P. aeruginosa was 35ºC (18). P. aeruginosa TMN produced di-RL at 37ºC as optimum temperature, above this there was decrease in production (24).…”

Section: Extraction Of Rlmentioning

confidence: 99%

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Induction of Rhamnolipid Production by Pseudomonas Aeruginosa A3 Using Chemical and Physical Mutagenic Factors

Faqri¹

2019

Iraqi J. Agric. Sci.

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This study was depend to select Pseudomonas aeruginosa A3 as a good producer of rhamnolipid (RL) biosurfactant after screening on agar plate where it was able to biosynthesize 4.3 g/L with emulsification index 52% and reducing the surface tension of water to 33.2 mN/m. Therefore, this study was aimed to increase the production of biosurfactant from selected isolate by exposure to several physical and chemical mutagenic factors like gamma radiation, nitrosoguanidine, ethyl methane sulfonate and lithium chloride. The results were shown that 0.2 g/L of nitrosoguanidine was the best mutant for increasing the production to about 2 folds (9.4 g/L) after 15 mins exposure to this material, as well as the emulsification index and surface tension of water were reached to 62% and 26.1 mN/m, respectively, comparing with non-mutant isolate. Also, the critical micelle concentration (CMC) and critical micelle dilution (CMD) of produced rhamnolipid were reached to 120 mg/L and 100 fold, respectively. The optimum conditions of RL production from mutant isolate were determined as 34ºC a best temperature, 6.5 optimum pH and incubation period of 108 h where the production was reached to 10.6 g/L and emulsification index 64% with surface tension of water 26 mN/m. characterization study of purified RL by using thin layer chromatography (TLC) analysis indicated that it was composed of a mixture of mono and di-rhamnolipid.

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Section: Extraction Of Rlsupporting

confidence: 89%

Section: Extraction Of Rlmentioning

confidence: 94%

Section: Extraction Of Rlmentioning

confidence: 99%

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Induction of Rhamnolipid Production by Pseudomonas Aeruginosa A3 Using Chemical and Physical Mutagenic Factors

Faqri¹

2019

Iraqi J. Agric. Sci.

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“…Rhamnolipid yield was further improved when 4.5% (v/v) oil was added initially during batch fermentation, and the trend regarding the influence of NaNO 3 concentration on rhamnolipid production was the same as that obtained at 3% (v/v) oil addition. The highest yield of rhamnolipid production was achieved equal to 29.5 g L −1 after 6 days; when the initial concentration of NaNO 3 was 8 g L −1 with oil addition (4.5%), which is higher than the values reported by several previous studies using this strain (13.7 and 12.3 g L −1 ) (Kaskatepe et al, ; Zhang et al, ). The lower rhamnolipid yield is probably due to the utilization of water‐soluble fraction in fish meal and solid stearic acid, which are not the best suitable carbon source for high‐performance rhamnolipid production (Ito and Inoue, ).…”

Section: Resultsmentioning

confidence: 51%

High‐Performance Production of Biosurfactant Rhamnolipid with Nitrogen Feeding

Jiang

et al. 2019

J Surfact & Detergents

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Nitrate is one of the most important factors for fermentative production of rhamnolipid, while it has not yet been conclusively demonstrated that nitrogen limitation or abundance is beneficial for improvement of rhamnolipid production. This study clarified that high concentration of NaNO 3 is conducive for high-performance production of rhamnolipid. An optimum rhamnolipid yield was achieved by 43.3 g L −1 with an initial concentration of NaNO 3 equal to 10 g L −1 during the regular fed-batch fermentation process by Pseudomonas aeruginosa ATCC 9027. Additionally, this rhamnolipid production was further improved to 61.2 g L −1 , which was two folds higher than that value obtained during batch fermentation, when the NaNO 3 concentration was maintained about 5 g L −1 . Furthermore, specific volume productivity of rhamnolipid was improved by over 30% in the presence of NaNO 3 at concentration ranging from 5 to 6 g L −1 during sequential fed-batch fermentation, resulting a high value of 0.4 and 0.59 g L −1 h −1 within one batch of sequential fed-batch fermentation for a period of 17 days by P. aeruginosa ATCC 9027 and PAO1, respectively. It seems that sustaining highconcentration NaNO 3 during fed-batch fermentation is advantageous for high-performance production of rhamnolipid.Keywords Rhamnolipid productivity Á Nitrogen Á Fedbatch fermentation J Surfact Deterg (2019) 22: 395-402.These two authors had equal contributions to this work.

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“…[4,5]. Previously, soybean, sunflower oils, technical glycerola by-product of biodiesel production were used for the rhamnolipid biosynthesis [6][7][8], the mathematical methods were applied for the synthesis optimization [9][10][11]. However, it is known, that the processes of biosurfactants allocation are up to 50-80% of the total cost of their production [2].…”

mentioning

confidence: 99%

Isolation of Surfactants Synthesized by the Pseudomonas Bacteria and Study of Their Properties

Pokynbroda¹,

Карпенко²,

Midyana³

et al. 2019

Innov Biosyst Bioeng

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Background. The important problem of biosurfactants production is biosynthesis optimization. But lack of effective isolation methods elaboration with simultaneous new costeffective products is the greatest weaknesses of existing technologies. Objective. The aim of the study is rational technology elaboration for isolation of biosurfactants obtained from strains Pseudomonas sp. PS-17 and P. fluorescens 8573. Investigation of the influence of different acids and temperatures on the efficiency of isolation of the surfactant products, study the properties of the obtained products. Determination of the possible directions of the use of supernatant obtained after precipitation of the biocomplexes (SPL) as a new inexpensive product. Methods. Rhamnolipid surfactant concentrate was precipitated from culture liquid supernatant (CLS) by acidification to pH 3-4 with acid solutions (HCl, H2SO4, H3PO4, HNO3, CH3COOH), kept at 100 C for 25 min, cooled to room temperature, centrifuged before the phase separation, the supernatant was decanted. The surface tension of the SPL was determined by du Noüy method (with a platinum ring). The emulsification index of biosurfactants was determined regarding mineral oil and sunflower oil. The RLs were isolated from the SPL by extraction with a mixture of ethyl acetate and isopropanol, their composition was determined by thin layer chromatography. Influence on plants was assessed by their morphometric parameters after presowing seed treatment. Results. The rational technology for surfactants isolation from strains Pseudomonas sp. PS-17 and P. fluorescens 8573 was developed It was shown that the suitable method of the isolation of the biosurfactants of Pseudomonas sp. PS-17 and P. fluorescens 8573 is acidic precipitation from CLS with heating. As a result, the product yield was increased by 20%, and the duration of the process was reduced. The physico-chemical properties of the SPLs after the isolation of biosurfactants from the CLS were studied. SPLs have been shown to be effective oil emulsifiers, foaming and wetting agents for various surfaces. It was shown that SPLs (at dilutions 1:10) do not exhibit phytotoxic effects and stimulate the growth of watercress. Conclusions. The new wasteless technology for Pseudomonas strains biosurfactants isolation has been proposed, which provides for the elimination of the extraction stage with solvents, as a result, the yield of the target products has been increased. Thus, the technology has economic and environmental advantages. It was shown that SPLs, being inexpensive and effective products, can be used in environmentally friendly technologies: in agriculture (for stimulation of plant growth), for remеdiation of contaminated soils, production of detergent compositions.

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Biosurfactant production by <italic>Pseudomonas aeruginosa</italic>in kefir and fish meal

Cited by 27 publications

References 23 publications

Induction of Rhamnolipid Production by Pseudomonas Aeruginosa A3 Using Chemical and Physical Mutagenic Factors

Induction of Rhamnolipid Production by Pseudomonas Aeruginosa A3 Using Chemical and Physical Mutagenic Factors

High‐Performance Production of Biosurfactant Rhamnolipid with Nitrogen Feeding

Isolation of Surfactants Synthesized by the Pseudomonas Bacteria and Study of Their Properties

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