2015
DOI: 10.1590/s1517-838246320120226
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Abstract: To facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was constructed. The alkane hydroxylase (alkB) gene of Pseudomonas putida GPo1 was constructed in a pCom8 expression vector, and the pCom8-GPo1 alkB plasmid was transformed into Escherichia coli DH5α. The AlkB protein was expressed by diesel oil induction and detected through SDS-polyacrylamide gel electrophoresis. The culture of the recombinant (pCom8-GPo1 alkB/E. coli DH5α) with the oil biodegradation bacterial consort… Show more

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Cited by 11 publications
(4 citation statements)
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“…It is the first time using AlkB to degrade n-alkane (n-dodecane) in a recombinant organism. The next year, the AlkB gene of P. putida GPo1 was constructed in a PCom8 expression vector, and the pCom8-GPo1 AlkB plasmid was transformed into E. coli DH5a [ 84 ]. The result showed that the culture of the recombinant E. coli with the petroleum biodegradation bacterial community increased the degradation ratio of diesel oil at 24 h from 31% to 50% [ 84 ].…”
Section: Engineered Microbial Chassismentioning
confidence: 99%
See 1 more Smart Citation
“…It is the first time using AlkB to degrade n-alkane (n-dodecane) in a recombinant organism. The next year, the AlkB gene of P. putida GPo1 was constructed in a PCom8 expression vector, and the pCom8-GPo1 AlkB plasmid was transformed into E. coli DH5a [ 84 ]. The result showed that the culture of the recombinant E. coli with the petroleum biodegradation bacterial community increased the degradation ratio of diesel oil at 24 h from 31% to 50% [ 84 ].…”
Section: Engineered Microbial Chassismentioning
confidence: 99%
“…The next year, the AlkB gene of P. putida GPo1 was constructed in a PCom8 expression vector, and the pCom8-GPo1 AlkB plasmid was transformed into E. coli DH5a [ 84 ]. The result showed that the culture of the recombinant E. coli with the petroleum biodegradation bacterial community increased the degradation ratio of diesel oil at 24 h from 31% to 50% [ 84 ]. On the other hand, other studies focused on ω-oxidation, which can convert alkanes to more valuable chemicals.…”
Section: Engineered Microbial Chassismentioning
confidence: 99%
“…Such genetically modified engineered microorganisms can degrade or assist in the degradation of complex compounds. Luo Q. et al (2015) constructed oil biodegradation bacteria to promote the biodegradation of diesel. The alkane hydroxylase (alkB) gene was introduced into Escherichia coli , giving it the ability to degrade diesel fuel.…”
Section: Current Status Of Biodegradation Of Complex Compounds By Mic...mentioning
confidence: 99%
“…(Nie et al 2014 ; Kadri et al 2018 ). Its overproduction has been achieved through overexpressing the all gene of both the gram-negative and gram-positive bacteria into another host; however, rubredoxin and rubredoxin reductase were reported as their essential cofactors (Luo et al 2015 ). It has been reported by Kadri et al ( 2018 ) and Al-Hawash et al ( 2018 ) that media engineering and selection of appropriate carbon sources improved AlkB specific activity in Alcanivorax borkumensis and Aspergillus sp.…”
Section: Introductionmentioning
confidence: 99%