Isolation and 2,4-D-degrading characteristics of Cupriavidus campinensis BJ71

Abstract

An indigenous bacterial strain capable of utilizing 2,4-dichlorophenoxyacetic acid as the sole carbon and energy source was isolated from a soil used for grown wheat with a long-term history of herbicide use in Beijing, China. The strain BJ71 was identified as Cupriavidus campinensis based on its 16S rRNA sequence analysis and morphological, physiological, and biochemical characteristics. The degradation characteristics of strain BJ71 were evaluated. The optimal conditions for 2,4-D degradation were as follows: pH 7.0, 30 °C, 3% (v/v) inoculum size, and an initial 2,4-D concentration of 350 mg L−1. Up to 99.57% of the 2,4-D was degraded under optimal conditions after 6 days of incubation. Strain BJ71 was also able to degrade quizalofop and fluroxypyr. This is the first report of a 2,4-D-degrader containing tfdA gene that can utilize these two herbicides. In a biodegradation experiment, 87.13% and 42.53% of 2,4-D (initial concentration, 350 mg kg−1) was degraded in non-sterile and sterilized soil inoculated with BJ71, respectively, after 14 days. The 2,4-D degradation was more rapid in a soil microcosm including BJ71 than in a soil microcosm without BJ71. These results indicate that strain BJ71 is a potential candidate for the bioremediation of soil contaminated with the herbicide 2,4-D.

Biosynthesis of Polyhydroxyalkanoates (PHAs) by the Valorization of Biomass and Synthetic Waste

“…These strains already have modified metabolism rates to sustain in oligotrophic conditions so they adapt to utilize the carbon available in plastic [ 33 ]. This shows an enhanced ability of indigenous bacteria to survive in nutrient-deficient conditions than non-indigenous bacteria [ 34 ]. In this research, the outcome of considerable differences in the PHA yields of bacteria isolated from plastic habitats can be due to the differences in the surrounding habitats of the plastic sources.…”

Agroecological aspects of application of Pseudomonas sp. DA1.2 in overcoming herbicidal stress in wheat

“…A decrease in the content of auxins could be due to the destruction of 2,4-D under the bacteria action. The ability of bacteria to destroy pesticides is known in the literature [14]. However, this assumption needs to be verified, given that the herbicide has been in contact with bacteria for a short time.…”

Impact of Herbicide 2, 4-Dichlorophenoxyacetic Acid Butyl Ester on Soil Nitrogen-transforming Bacterial Populations in Two Soils

“…Zabaloy et al (2010) thought that low rates of 2, 4-D did not affect heterotrophic bacteria counts, our experimental results supported these conclusions, addition of 2, 4-Dbe increased numbers of heterotrophic nitrifying bacteria. Because González et al (2012) and Han et al (2015) have isolated an indigenous bacterial strain which utilize 2, 4-D as the sole carbon and energy source from a polluted river and from a soil of growing wheat with a longterm history of herbicide use, respectively. However, nitrifying bacteria appeared to be the most sensitive microorganisms to napropamide (Cycoń et al, 2013), the conclusion also was supported by our experimental results with herbicide 2, 4-D.…”