Adhesion, biofilm and genotypic characteristics of antimicrobial resistant Escherichia coli isolates

Cergole-Novella, Maria Cecília; Pignatari, Antônio Carlos Campos; Guth, Beatriz E. C.

doi:10.1590/s1517-838246120140077

Cited by 17 publications

(9 citation statements)

References 29 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was found that fimH, hlyA, traT, csgA, and crl genes were more common among Uropathogenic E. coli compared to isolates of other ExPEC and intestinal isolates indicating that uropathogenic isolates were more virulent than other tested isolates. Cergole-Novella et al 60 showed that there was an association among fimH, crl, csg, traT and colV with bla −CTX-M-15 in E. coli isolated from gastroenteritis which was in agreement with our results.…”

Section: Discussionsupporting

confidence: 93%

“…3,16 In our study, Multi-drug resistance (MDR) (resistance to ≥3 antimicrobials of different classes) to most of the tested antibiotics was more common among urine and blood E. coli isolates especially to cefepime (100% resistance) and the other tested cephalosporins. Ciprofloxacin resistance was observed mostly among urine and blood isolates (50% and 66.6%, respectively) which in agreement with Raeispour and Ranjbar, 67 Abdi et al 68 and Hashemizadeh et al 69 Cergole-Novella et al 60 showed that fimH, crl, csg, traT and colV were common among all tested E. coli obtained from different sources (urinary tract infection, septicemia, respiratory infection and gastroenteritis) with 100% prevalence of fimH, crl, csg genes among all isolates followed by traT (83.3%). Also, they showed that biofilm production was observed among isolates from gastroenteritis, sepsis and UTI.…”

Section: Discussionsupporting

confidence: 80%

See 1 more Smart Citation

Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic E. coli Isolated from Egyptian Patients with Different Clinical Infections

El-Baky¹,

Ibrahim²,

Mohamed³

et al. 2020

IDR

View full text Add to dashboard Cite

Introduction: Escherichia (E.) coli can cause intestinal and extra-intestinal infections which ranged from mild to life-threatening infections. The severity of infection is a product of many factors including virulence properties and antimicrobial resistance. Objectives: To determine the antibiotic resistance pattern, the distribution of virulence factors and their association with one another and with some selected resistance genes. Methods: Virulence properties were analyzed phenotypically while antimicrobial susceptibility was tested by Kirby-Bauer agar disc diffusion method. In addition, 64 E. coli isolates were tested for 6 colicin genes, fimH, hlyA, traT, csgA, crl virulence genes and bla −CTX-M-15 , bla −oxa-2 , and bla −oxa-10 resistance genes by polymerase chain reaction (PCR). Results: Extra-intestinal pathogenic E. coli isolated from urine and blood samples represented a battery of virulence factors and resistance genes with a great ability to produce biofilm. Also, a significant association (P<0.05) among most of the tested colicin, virulence and resistance genes was observed. The observed associations indicate the importance and contribution of the tested factors in the establishment and the progress of infection especially with Extra-intestinal E. coli (ExPEC) which is considered a great challenging health problem. Conclusion: There is a need for studying how to control these factors to decrease the rate and the severity of infections. The relationship between virulence factors and resistance genes is complex and needs more studies that should be specific for each area.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 80%

Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic E. coli Isolated from Egyptian Patients with Different Clinical Infections

El-Baky¹,

Ibrahim²,

Mohamed³

et al. 2020

IDR

View full text Add to dashboard Cite

show abstract

“…In Brazil, around 58% of ExPEC resistant to antimicrobials isolated from humans were reported as adherent to Caco-2 cells (Cergole-Novella et al 2015). In the present study, among the 15 MDR E. coli phenotypically analyzed in vitro, 100% showed ability to adhesion in Caco-2 cells.…”

Section: Discussionsupporting

confidence: 45%

Virulence potential of commensal multidrug resistant Escherichia coli isolated from poultry in Brazil

Ferreira

Filho

Kuaye

et al. 2018

Infection, Genetics and Evolution

View full text Add to dashboard Cite

There is an increasing number of reports worldwide about multidrug resistance (MDR) with potential of ExPEC in commensal E. coli. The present study evaluated the potential ExPEC in selected 44 MDR E.coli isolates, collected from livestock. ExPEC isolates were characterized by analysis of five main groups of virulence genes (papA and/or papC, sfa and/or foc, afa and/or dra, kpsMT II and iutA). We also determined the increased virulence potential analyzing other 29 virulence genes, the epidemiology of these isolates. Additionally, fifteen ExPEC isolates were selected to evaluate the adhesion and invasion capacity in vitro using Caco-2 cells. Based on the analysis of the five main virulence genes, 72.7% (32/44) strains were classified as ExPEC. The presence of each gene was iutA 88.6%, KpsMT II 70.4%, papC 25%, sfa/focDE 4.5%; afa/draBC genes were not found. All E. coli isolates were classified into: phylogenetic groups A (34%), B1 (10%), B2 (20%), and D (36%). MLST revealed 7 different STs among isolates, including a new ST identified (ST5687). The in vitro assay in Caco-2 cells showed that all isolates were capable to adhere or invade the epithelial cells, although this occurred at variable levels. The ExPEC isolate LO122 reached similar levels of invasion to the positive control strain Salmonella Typhimurium LT2. These results showed that the apparently commensal microbiota of poultry harbors MDR ExPEC isolates with high adhesion and invasion potential.

show abstract

“…crl, csg, cvaC, fimA, fimH, iutA, ompC, ompF, sfaS, traT, yidC, etc. responsible for the production of biofilm [24, 34, 35].…”

Section: Discussionmentioning

confidence: 99%

Extended spectrum β-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in Nepal

Shrestha

Khanal

Poudel

et al. 2019

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

BackgroundUrinary tract infection (UTI) is one of the frequently diagnosed infectious diseases which is caused mainly by Escherichia coli. E. coli confers resistance against the two major classes of antibiotics due to the production of extended spectrum β-lactamase enzymes (ESBL), biofilm, etc. Biofilm produced by uropathogenic E. coli (UPEC) protects from host immune system and prevent entry of antimicrobial compounds. The main objective of this cross-sectional study was to determine the correlation of biofilm production and antibiotic resistance as well as to characterize the pgaA and pgaC genes responsible for biofilm formation among uropathogenic ESBL producing E. coli.MethodsA total of 1977 mid-stream urine samples were examined and cultured for bacterial strain identification. ESBL was detected by combined disc method following CLSI whereas biofilm formation was analyzed by semi-quantitative method. Furthermore, the pgaA and pgaC genes responsible for biofilm formation in UPEC were detected by multiplex PCR. All the statistical analyses were done via IBM SPSS Statistics 21 where Pearson’s correlation test were used to determine correlation (−1 ≥ r ≤ 1).ResultsE. coli was the predominant causative agent, which accounted 159 (59.3%) of the Gram-negative bacteria, where 81 (50.9%) E. coli strains were found to be ESBL producers. In addition, 86 (54.1%) E. coli strains were found to be biofilm producers. Both the pgaA and pgaC genes were detected in 45 (93.7%) the UPEC isolates, which were both biofilm and ESBL producers. Moreover, there was a positive correlation between biofilm and ESBL production.ConclusionThe analyses presented weak positive correlation between biofilm and ESBL production in which biofilm producing UPEC harbors both pgaA and pgaC genes responsible for biofilm formation.

show abstract

Adhesion, biofilm and genotypic characteristics of antimicrobial resistant Escherichia coli isolates

Cited by 17 publications

References 29 publications

<p>Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic <em>E. coli</em> Isolated from Egyptian Patients with Different Clinical Infections</p>

<p>Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic <em>E. coli</em> Isolated from Egyptian Patients with Different Clinical Infections</p>

Virulence potential of commensal multidrug resistant Escherichia coli isolated from poultry in Brazil

Extended spectrum β-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in Nepal

Contact Info

Product

Resources

About