2015
DOI: 10.1590/s1517-838246120130851
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Abstract: A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.

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Cited by 10 publications
(17 citation statements)
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“…Though a rapid high-throughput colorimetric method for screening nitrilase-producing microorganisms using pH sensitive indicators was developed by Banerjee et al [21], the method has the lacuna of differentiating between nitrilase and nitrile hydratase in bacteria. Screening for both the enzymes simultaneously under dye based method and Nesslarization method have not been reported so far and a first report is presented here where a simple and rapid pH sensitive indicators are used to evaluate the activity of nitrile hydrolyzing enzymes by exploiting the sensitivity of amidase to the substrate acrylonitrile [40]. Detection of ammonia released due to nitrilase activity by Nesslarization method directly determines the activity of nitrilase, an additional confirmatory method.…”
Section: Resultsmentioning
confidence: 99%
“…Though a rapid high-throughput colorimetric method for screening nitrilase-producing microorganisms using pH sensitive indicators was developed by Banerjee et al [21], the method has the lacuna of differentiating between nitrilase and nitrile hydratase in bacteria. Screening for both the enzymes simultaneously under dye based method and Nesslarization method have not been reported so far and a first report is presented here where a simple and rapid pH sensitive indicators are used to evaluate the activity of nitrile hydrolyzing enzymes by exploiting the sensitivity of amidase to the substrate acrylonitrile [40]. Detection of ammonia released due to nitrilase activity by Nesslarization method directly determines the activity of nitrilase, an additional confirmatory method.…”
Section: Resultsmentioning
confidence: 99%
“…Angelini et al . () developed a high‐throughput screening assay to distinguish microorganisms containing nitrilases and those possessing nitrile hydratases. A Banerjee‐modified colorimetric and pH‐sensitive assay was used with an amidase inhibitor in microplates using mandelonitrile as a substrate.…”
Section: Nature's Task Force: Screening Biocatalysts From the Environmentioning
confidence: 98%
“…TFNG‐AM is the product of flonicamid hydration pathway, which require the activity of NHase (Angelini et al . 2015). The NHase expression was induced in E. coli Rosetta (DE3) overexpressing Aminobacter sp.…”
Section: Resultsmentioning
confidence: 99%