2014
DOI: 10.1590/s1517-83822014005000026
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Manganese peroxidase (MnP) was produced from white rot edible mushroom Pleurotus ostreatus on the culture filtrate. The enzyme was purified to homogeneity using (NH4)2SO4 precipitation, DEAE-Sepharose and Sephadex G-100 column chromatography. The final enzyme activity achieved 81 U mL−1, specific activity 78 U mg−1 with purification fold of 130 and recovery 1.2% of the crude enzyme. SDS-PAGE indicated that the pure enzyme have a molecular mass of approximately 42 kDa. The optimum pH was between 4–5 and the opt… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4

Citation Types

1
56
1
2

Year Published

2016
2016
2023
2023

Publication Types

Select...
6
2

Relationship

0
8

Authors

Journals

citations
Cited by 96 publications
(60 citation statements)
references
References 40 publications
1
56
1
2
Order By: Relevance
“…However, the exact reaction mechanism has not yet been determined, and there is no information about the residues involved in the enzyme‐catalyzed reaction. Similar reaction mechanism was proposed for AFB1 detoxification catalyzed by manganese peroxidase, but with H 2 O 2 as a reactant instead of a product …”
Section: Introductionmentioning
confidence: 71%
See 1 more Smart Citation
“…However, the exact reaction mechanism has not yet been determined, and there is no information about the residues involved in the enzyme‐catalyzed reaction. Similar reaction mechanism was proposed for AFB1 detoxification catalyzed by manganese peroxidase, but with H 2 O 2 as a reactant instead of a product …”
Section: Introductionmentioning
confidence: 71%
“…Similar reaction mechanism was proposed for AFB1 detoxification catalyzed by manganese peroxidase, but with H 2 O 2 as a reactant instead of a product. 27,28 Although a certain sequence similarity (up to 42%) with another enzyme, dipeptidyl peptidase III (DPP III), was noticed in earlier articles, 25,29 no tests for peptidase activity were performed at the time. The crystal structure of AFO, recently determined by Xu et al…”
mentioning
confidence: 99%
“…The use of microorganisms has been studied for their special potential to control AFs. The microbial strategy is related to the adsorption or the enzymatic degradation of aflatoxins via bacteria (Ananthi, Subramanian, & Arun, 2016;Bovo, Corassin, Rosim, & de Oliveira, 2013;Samuel, Sivaramakrishna, & Mehta, 2014), fungi (Shcherbakova, Statsyuk, Mikityuk, Nazarova, & Dzhavakhiya, 2015;Yehia, 2014), and yeast (Deepak, Jhanvi, & Anu Appaiah, 2015;Gonçalves, Rosim, Oliveira, & Corassin, 2014).…”
Section: Introductionmentioning
confidence: 99%
“…(Krifaton et al, 2011;Samuel et al, 2014;Sangare et al, 2014). Likewise, a number of fungal strains such as Armillariella tabescens (Liu et al, 2001), Phanerochaete sordida (Wang et al, 2011), Pleurotus ostreatus (Yehia, 2014) and a host of others (Adebo et al, 2015;Alberts et al, 2009;Wu et al, 2009) have been found to transform AFB 1 to less toxic products.…”
Section: Introductionmentioning
confidence: 99%