2013
DOI: 10.1590/s1517-83822014005000005
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Abstract: Clavulanic acid (CA) is a powerful inhibitor of the beta-lactamases, enzymes produced by bacteria resistants to penicillin and cefalosporin. This molecule is produced industrially by strains of Streptomyces clavuligerus in complex media which carbon and nitrogen resources are supplied by inexpensive compounds still providing high productivity. The genetic production improvement using physical and chemical mutagenic agents is an important strategy in programs of industrial production development of bioactive me… Show more

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Cited by 7 publications
(6 citation statements)
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“…The mutant strain obtained 812mg L-1 concentration in 72 h of fermentation, while the wild-type obtained 639.7.1mg L-1 at the same time ( Figure 1). This concentration of CA in the mutant strain was similar at our previous study, 12 in which only the chemical mutagenesis was used. The significant increase in biomass in the mutant strain compared to wild type (Figure 2), suggests that the mutations occurred in the genes which are involved in the primary metabolites responsible for growth.…”
Section: Resultssupporting
confidence: 62%
See 1 more Smart Citation
“…The mutant strain obtained 812mg L-1 concentration in 72 h of fermentation, while the wild-type obtained 639.7.1mg L-1 at the same time ( Figure 1). This concentration of CA in the mutant strain was similar at our previous study, 12 in which only the chemical mutagenesis was used. The significant increase in biomass in the mutant strain compared to wild type (Figure 2), suggests that the mutations occurred in the genes which are involved in the primary metabolites responsible for growth.…”
Section: Resultssupporting
confidence: 62%
“…This specific medium was used due to good results in our laboratory investigating production of CA with mutant strains in a previous study. 12…”
Section: Culture Mediamentioning
confidence: 99%
“…Several carbon sources (glycerol, starch, sucrose, and lipid) have been used to produce CA [ 70 , 95 , 101 , 102 , 103 ]. Glycerol plays an important role in CA production as the C3 precursor of the molecule [ 104 ].…”
Section: Production Of β-Lactamase Inhibitorsmentioning
confidence: 99%
“…After 36h of fermentation, the lipase hydrolytic activity was 3000UL -1 , measured at pH 9.0 and 37°C by using p-nitrophenyl palmitate (pNPP) as substrate. In another study with S. clavuligerus, Vasconcelos et al 13 compared the extracellular lipase enzymatic activity by spectrophotometry using pNPP as substrate between wild-type and mutant MMS 150 strain, using the complex culture medium proposed by. 14 When cultivated in complex medium, the mutant strain presented less lipolytic activity in comparison with the wild-type.…”
Section: Lipase Production and Activity In Streptomycesmentioning
confidence: 99%